Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.)
Wheat leaf rust (LR) causes significant yield losses worldwide. In Egypt, resistant cultivars began to lose their efficiency in leaf rust resistance. Therefore, a diverse spring wheat panel was evaluated at the seedling stage to identify new sources of broad-spectrum seedling resistance against the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.921230/full |
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| author | Amira M. I. Mourad Amira M. I. Mourad Ibrahim S. Draz Ghady E. Omar Andreas Börner Samar M. Esmail |
| author_facet | Amira M. I. Mourad Amira M. I. Mourad Ibrahim S. Draz Ghady E. Omar Andreas Börner Samar M. Esmail |
| author_sort | Amira M. I. Mourad |
| collection | DOAJ |
| description | Wheat leaf rust (LR) causes significant yield losses worldwide. In Egypt, resistant cultivars began to lose their efficiency in leaf rust resistance. Therefore, a diverse spring wheat panel was evaluated at the seedling stage to identify new sources of broad-spectrum seedling resistance against the Egyptian Puccinia triticina (Pt) races. In three different experiments, seedling evaluation was done using Pt spores collected from different fields and growing seasons. Highly significant differences were found among experiments confirming the presence of different races population in each experiment. Highly significant differences were found among the tested genotypes confirming the ability to select superior genotypes. Genome-wide association study (GWAS) was conducted for each experiment and a set of 87 markers located within 48 gene models were identified. The identified gene models were associated with disease resistance in wheat. Five gene models were identified to resist all Pt races in at least two experiments and could be identified as stable genes under Egyptian conditions. Ten genotypes from five different countries were stable against all the tested Pt races but showed different degrees of resistance. |
| first_indexed | 2024-04-13T16:34:22Z |
| format | Article |
| id | doaj.art-9fef1d62a9a5465191177886e2980c4b |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T16:34:22Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9fef1d62a9a5465191177886e2980c4b2022-12-22T02:39:28ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.921230921230Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.)Amira M. I. Mourad0Amira M. I. Mourad1Ibrahim S. Draz2Ghady E. Omar3Andreas Börner4Samar M. Esmail5Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyDepartment of Agronomy, Faculty of Agriculture, Assiut University, Assiut, EgyptWheat Disease Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, EgyptWheat Disease Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, EgyptLeibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, GermanyWheat Disease Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, EgyptWheat leaf rust (LR) causes significant yield losses worldwide. In Egypt, resistant cultivars began to lose their efficiency in leaf rust resistance. Therefore, a diverse spring wheat panel was evaluated at the seedling stage to identify new sources of broad-spectrum seedling resistance against the Egyptian Puccinia triticina (Pt) races. In three different experiments, seedling evaluation was done using Pt spores collected from different fields and growing seasons. Highly significant differences were found among experiments confirming the presence of different races population in each experiment. Highly significant differences were found among the tested genotypes confirming the ability to select superior genotypes. Genome-wide association study (GWAS) was conducted for each experiment and a set of 87 markers located within 48 gene models were identified. The identified gene models were associated with disease resistance in wheat. Five gene models were identified to resist all Pt races in at least two experiments and could be identified as stable genes under Egyptian conditions. Ten genotypes from five different countries were stable against all the tested Pt races but showed different degrees of resistance.https://www.frontiersin.org/articles/10.3389/fpls.2022.921230/fullleaf rustseedling resistancebroad spectrum resistancegenome-wide association studygene modelsgene-network |
| spellingShingle | Amira M. I. Mourad Amira M. I. Mourad Ibrahim S. Draz Ghady E. Omar Andreas Börner Samar M. Esmail Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) Frontiers in Plant Science leaf rust seedling resistance broad spectrum resistance genome-wide association study gene models gene-network |
| title | Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) |
| title_full | Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) |
| title_fullStr | Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) |
| title_short | Genome-Wide Screening of Broad-Spectrum Resistance to Leaf Rust (Puccinia triticina Eriks) in Spring Wheat (Triticum aestivum L.) |
| title_sort | genome wide screening of broad spectrum resistance to leaf rust puccinia triticina eriks in spring wheat triticum aestivum l |
| topic | leaf rust seedling resistance broad spectrum resistance genome-wide association study gene models gene-network |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.921230/full |
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