Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles
Antioxidant capacity of poorly soluble natural antioxidant kaempferol, in particular free or loaded in two types of cationic micelles, was studied on non-enzyme induced lipid peroxidation (LPO) in vitro. The micelles were based on triblock copolymers - poly(2-(dimethylamino)ethyl methacrylate-b-poly...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-01-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/77678/download/pdf/ |
| _version_ | 1811275407033368576 |
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| author | Denitsa Aluani Magdalena Kondeva-Burdina Alexandra Tosheva Krassimira Yoncheva Virginia Tzankova |
| author_facet | Denitsa Aluani Magdalena Kondeva-Burdina Alexandra Tosheva Krassimira Yoncheva Virginia Tzankova |
| author_sort | Denitsa Aluani |
| collection | DOAJ |
| description | Antioxidant capacity of poorly soluble natural antioxidant kaempferol, in particular free or loaded in two types of cationic micelles, was studied on non-enzyme induced lipid peroxidation (LPO) in vitro. The micelles were based on triblock copolymers - poly(2-(dimethylamino)ethyl methacrylate-b-poly(propylene oxide)-b-poly(2-(dimethylamino)ethyl methacrylate (PDMAEMA-PPO-PDMAEMA) and poly(2-(dimethylamino)ethyl methacrylate-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate (PDMAEMA-PCL-PDMAEMA). The lipid peroxidation was induced by incubating of rat liver microsomes with iron sulphate and ascorbic acid (Fe2+/AA). The effect of free and micellar kaempferol (at concentrations 25, 50 and 75 μg/ml) was assessed after 20 min incubation time. In the non-enzyme lipid peroxidation model, the kaempferol-loaded micelles significantly decreased the formation of malondialdehyde (MDA). The effect of kaempferol loaded in PDMAEMA-PCL-PDMAEMA micelles was more pronounced, showing an improved antioxidant activity in the conditions of oxidative stress and lipid peroxidation in vitro. |
| first_indexed | 2024-04-12T23:38:26Z |
| format | Article |
| id | doaj.art-9ff20bd9c0424c1fa401be61aa2cd0bf |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-04-12T23:38:26Z |
| publishDate | 2022-01-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-9ff20bd9c0424c1fa401be61aa2cd0bf2022-12-22T03:12:04ZengPensoft PublishersPharmacia2603-557X2022-01-01691252910.3897/pharmacia.69.e7767877678Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micellesDenitsa Aluani0Magdalena Kondeva-Burdina1Alexandra Tosheva2Krassimira Yoncheva3Virginia Tzankova4Medical University of SofiaMedical University of SofiaMedical University of SofiaMedical University of SofiaMedical University of SofiaAntioxidant capacity of poorly soluble natural antioxidant kaempferol, in particular free or loaded in two types of cationic micelles, was studied on non-enzyme induced lipid peroxidation (LPO) in vitro. The micelles were based on triblock copolymers - poly(2-(dimethylamino)ethyl methacrylate-b-poly(propylene oxide)-b-poly(2-(dimethylamino)ethyl methacrylate (PDMAEMA-PPO-PDMAEMA) and poly(2-(dimethylamino)ethyl methacrylate-b-poly(ε-caprolactone)-b-poly(2-(dimethylamino)ethyl methacrylate (PDMAEMA-PCL-PDMAEMA). The lipid peroxidation was induced by incubating of rat liver microsomes with iron sulphate and ascorbic acid (Fe2+/AA). The effect of free and micellar kaempferol (at concentrations 25, 50 and 75 μg/ml) was assessed after 20 min incubation time. In the non-enzyme lipid peroxidation model, the kaempferol-loaded micelles significantly decreased the formation of malondialdehyde (MDA). The effect of kaempferol loaded in PDMAEMA-PCL-PDMAEMA micelles was more pronounced, showing an improved antioxidant activity in the conditions of oxidative stress and lipid peroxidation in vitro.https://pharmacia.pensoft.net/article/77678/download/pdf/ |
| spellingShingle | Denitsa Aluani Magdalena Kondeva-Burdina Alexandra Tosheva Krassimira Yoncheva Virginia Tzankova Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles Pharmacia |
| title | Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| title_full | Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| title_fullStr | Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| title_full_unstemmed | Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| title_short | Improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| title_sort | improvement of in vitro antioxidant activity of kaempferol by encapsulation in copolymer micelles |
| url | https://pharmacia.pensoft.net/article/77678/download/pdf/ |
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