Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses
Calmodulin-binding transcription activators (CAMTAs) are evolutionarily conserved transcription factors and have multi-functions in plant development and stress response. However, identification and functional analysis of tea plant (Camellia sinensis) CAMTA genes (CsCAMTAs) are still lacking. Here,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.910768/full |
| _version_ | 1811233510013272064 |
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| author | Qiying Zhou Qiying Zhou Qiying Zhou Mingwei Zhao Mingwei Zhao Mingwei Zhao Feng Xing Feng Xing Feng Xing Guangzhi Mao Guangzhi Mao Guangzhi Mao Yijia Wang Yijia Wang Yijia Wang Yafeng Dai Yafeng Dai Yafeng Dai Minghui Niu Minghui Niu Minghui Niu Hongyu Yuan Hongyu Yuan Hongyu Yuan |
| author_facet | Qiying Zhou Qiying Zhou Qiying Zhou Mingwei Zhao Mingwei Zhao Mingwei Zhao Feng Xing Feng Xing Feng Xing Guangzhi Mao Guangzhi Mao Guangzhi Mao Yijia Wang Yijia Wang Yijia Wang Yafeng Dai Yafeng Dai Yafeng Dai Minghui Niu Minghui Niu Minghui Niu Hongyu Yuan Hongyu Yuan Hongyu Yuan |
| author_sort | Qiying Zhou |
| collection | DOAJ |
| description | Calmodulin-binding transcription activators (CAMTAs) are evolutionarily conserved transcription factors and have multi-functions in plant development and stress response. However, identification and functional analysis of tea plant (Camellia sinensis) CAMTA genes (CsCAMTAs) are still lacking. Here, five CsCAMTAs were identified from tea plant genomic database. Their gene structures were similar except CsCAMTA2, and protein domains were conserved. Phylogenetic relationship classified the CsCAMTAs into three groups, CsCAMTA2 was in group I, and CsCAMTA1, 3 and CsCAMTA4, 5 were, respectively, in groups II and III. Analysis showed that stress and phytohormone response-related cis-elements were distributed in the promoters of CsCAMTA genes. Expression analysis showed that CsCAMTAs were differentially expressed in different organs and under various stress treatments of tea plants. Three-hundred and four hundred-one positive co-expressed genes of CsCAMTAs were identified under cold and drought, respectively. CsCAMTAs and their co-expressed genes constituted five independent co-expression networks. KEGG enrichment analysis of CsCAMTAs and the co-expressed genes revealed that hormone regulation, transcriptional regulation, and protein processing-related pathways were enriched under cold treatment, while pathways like hormone metabolism, lipid metabolism, and carbon metabolism were enriched under drought treatment. Protein interaction network analysis suggested that CsCAMTAs could bind (G/A/C)CGCG(C/G/T) or (A/C)CGTGT cis element in the target gene promoters, and transcriptional regulation might be the main way of CsCAMTA-mediated functional regulation. The study establishes a foundation for further function studies of CsCAMTA genes in stress response. |
| first_indexed | 2024-04-12T11:21:15Z |
| format | Article |
| id | doaj.art-9ffb8ca4ed39420eaaa04143cbb61afd |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T11:21:15Z |
| publishDate | 2022-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-9ffb8ca4ed39420eaaa04143cbb61afd2022-12-22T03:35:21ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.910768910768Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress ResponsesQiying Zhou0Qiying Zhou1Qiying Zhou2Mingwei Zhao3Mingwei Zhao4Mingwei Zhao5Feng Xing6Feng Xing7Feng Xing8Guangzhi Mao9Guangzhi Mao10Guangzhi Mao11Yijia Wang12Yijia Wang13Yijia Wang14Yafeng Dai15Yafeng Dai16Yafeng Dai17Minghui Niu18Minghui Niu19Minghui Niu20Hongyu Yuan21Hongyu Yuan22Hongyu Yuan23Henan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaHenan Key Laboratory of Tea Plant Biology, Xinyang Normal University, Xinyang, ChinaHenan Engineering Research Center of Tea Deep-Processing, Xinyang Normal University, Xinyang, ChinaInstitute for Conservation and Utilization of Agro-Bioresources in Dabie Mountains, Xinyang Normal University, Xinyang, ChinaCalmodulin-binding transcription activators (CAMTAs) are evolutionarily conserved transcription factors and have multi-functions in plant development and stress response. However, identification and functional analysis of tea plant (Camellia sinensis) CAMTA genes (CsCAMTAs) are still lacking. Here, five CsCAMTAs were identified from tea plant genomic database. Their gene structures were similar except CsCAMTA2, and protein domains were conserved. Phylogenetic relationship classified the CsCAMTAs into three groups, CsCAMTA2 was in group I, and CsCAMTA1, 3 and CsCAMTA4, 5 were, respectively, in groups II and III. Analysis showed that stress and phytohormone response-related cis-elements were distributed in the promoters of CsCAMTA genes. Expression analysis showed that CsCAMTAs were differentially expressed in different organs and under various stress treatments of tea plants. Three-hundred and four hundred-one positive co-expressed genes of CsCAMTAs were identified under cold and drought, respectively. CsCAMTAs and their co-expressed genes constituted five independent co-expression networks. KEGG enrichment analysis of CsCAMTAs and the co-expressed genes revealed that hormone regulation, transcriptional regulation, and protein processing-related pathways were enriched under cold treatment, while pathways like hormone metabolism, lipid metabolism, and carbon metabolism were enriched under drought treatment. Protein interaction network analysis suggested that CsCAMTAs could bind (G/A/C)CGCG(C/G/T) or (A/C)CGTGT cis element in the target gene promoters, and transcriptional regulation might be the main way of CsCAMTA-mediated functional regulation. The study establishes a foundation for further function studies of CsCAMTA genes in stress response.https://www.frontiersin.org/articles/10.3389/fpls.2022.910768/fulltea plantgenome-wideexpression analysisCAMTAgene family |
| spellingShingle | Qiying Zhou Qiying Zhou Qiying Zhou Mingwei Zhao Mingwei Zhao Mingwei Zhao Feng Xing Feng Xing Feng Xing Guangzhi Mao Guangzhi Mao Guangzhi Mao Yijia Wang Yijia Wang Yijia Wang Yafeng Dai Yafeng Dai Yafeng Dai Minghui Niu Minghui Niu Minghui Niu Hongyu Yuan Hongyu Yuan Hongyu Yuan Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses Frontiers in Plant Science tea plant genome-wide expression analysis CAMTA gene family |
| title | Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses |
| title_full | Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses |
| title_fullStr | Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses |
| title_full_unstemmed | Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses |
| title_short | Identification and Expression Analysis of CAMTA Genes in Tea Plant Reveal Their Complex Regulatory Role in Stress Responses |
| title_sort | identification and expression analysis of camta genes in tea plant reveal their complex regulatory role in stress responses |
| topic | tea plant genome-wide expression analysis CAMTA gene family |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.910768/full |
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