Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers
Aim: This study was conducted to study the genetic and population structure of local (Awassi) and exotic (Romanov, Charollais, Assaf, Awassi, and Suffolk) sheep breeds in Jordan using eight microsatellite markers. Materials and Methods: A total of 125 sheep were used (25 from each breed) in the s...
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| Format: | Article |
| Language: | English |
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Veterinary World
2018-06-01
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| Series: | Veterinary World |
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| Online Access: | http://www.veterinaryworld.org/Vol.11/June-2018/7.pdf |
| _version_ | 1818741473097547776 |
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| author | Khaleel I. Jawasreh Mustafa M. Ababneh Zuhair Bani Ismail Abdel Mon'em Bani Younes Ibrahem Al Sukhni |
| author_facet | Khaleel I. Jawasreh Mustafa M. Ababneh Zuhair Bani Ismail Abdel Mon'em Bani Younes Ibrahem Al Sukhni |
| author_sort | Khaleel I. Jawasreh |
| collection | DOAJ |
| description | Aim: This study was conducted to study the genetic and population structure of local (Awassi) and exotic (Romanov, Charollais, Assaf, Awassi, and Suffolk) sheep breeds in Jordan using eight microsatellite markers.
Materials and Methods: A total of 125 sheep were used (25 from each breed) in the study. The number of alleles (A), the mean values of observed (Ho) and expected (He) heterozygosity, polymorphism information content (PIC), fixation index as a measure of heterozygote deficiency or excess, and Hardy-Weinberg equilibrium (HWE) were analyzed using PopGen and CERVUS softwares. Nei's standard genetic distances among breeds and dendrogram of unweighted pair group method with arithmetic mean (UPGMA) were calculated and constructed using PopGen software.
Results: A total of 40 alleles were detected with an average number of alleles of 5. The mean Ho value was higher than the mean He value for all breeds. Awassi breed showed the highest average PIC value while Romanov had the lowest. There was a significant (p<0.05) deviation from HWE at each locus within and between breeds. Deviations from HWE were found to be highly significant for all markers except OARFCP304 locus. The genetic distance estimates revealed a close relationship between Romanov and Charollais and between Awassi and Charollais. In the UPGMA dendrogram, Charollais, Romanov, and Awassi breeds were placed together in one main cluster while Assaf was in a different subcluster. Awassi was placed alone in a second main cluster.
Conclusion: Results of this study offer insight toward the genetic conservation of the studied breeds and a base on which breeding plans can be made. |
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| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-18T01:57:11Z |
| publishDate | 2018-06-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-9ffe0a603b884be29b70e37c92c86d0b2022-12-21T21:24:54ZengVeterinary WorldVeterinary World0972-89882231-09162018-06-0111677878110.14202/vetworld.2018.778-781Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markersKhaleel I. Jawasreh0Mustafa M. Ababneh1Zuhair Bani Ismail2Abdel Mon'em Bani Younes3Ibrahem Al Sukhni4Department Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, P. O. Box 2210, Jordan.Department of Basic Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, P. O. Box 2210, Jordan.Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, P. O. Box 2210, Jordan.Department Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, P. O. Box 2210, Jordan.Department Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, P. O. Box 2210, Jordan.Aim: This study was conducted to study the genetic and population structure of local (Awassi) and exotic (Romanov, Charollais, Assaf, Awassi, and Suffolk) sheep breeds in Jordan using eight microsatellite markers. Materials and Methods: A total of 125 sheep were used (25 from each breed) in the study. The number of alleles (A), the mean values of observed (Ho) and expected (He) heterozygosity, polymorphism information content (PIC), fixation index as a measure of heterozygote deficiency or excess, and Hardy-Weinberg equilibrium (HWE) were analyzed using PopGen and CERVUS softwares. Nei's standard genetic distances among breeds and dendrogram of unweighted pair group method with arithmetic mean (UPGMA) were calculated and constructed using PopGen software. Results: A total of 40 alleles were detected with an average number of alleles of 5. The mean Ho value was higher than the mean He value for all breeds. Awassi breed showed the highest average PIC value while Romanov had the lowest. There was a significant (p<0.05) deviation from HWE at each locus within and between breeds. Deviations from HWE were found to be highly significant for all markers except OARFCP304 locus. The genetic distance estimates revealed a close relationship between Romanov and Charollais and between Awassi and Charollais. In the UPGMA dendrogram, Charollais, Romanov, and Awassi breeds were placed together in one main cluster while Assaf was in a different subcluster. Awassi was placed alone in a second main cluster. Conclusion: Results of this study offer insight toward the genetic conservation of the studied breeds and a base on which breeding plans can be made.http://www.veterinaryworld.org/Vol.11/June-2018/7.pdfgenetic diversityJordanmicrosatellitessheep breeds |
| spellingShingle | Khaleel I. Jawasreh Mustafa M. Ababneh Zuhair Bani Ismail Abdel Mon'em Bani Younes Ibrahem Al Sukhni Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers Veterinary World genetic diversity Jordan microsatellites sheep breeds |
| title | Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers |
| title_full | Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers |
| title_fullStr | Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers |
| title_full_unstemmed | Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers |
| title_short | Genetic diversity and population structure of local and exotic sheep breeds in Jordan using microsatellites markers |
| title_sort | genetic diversity and population structure of local and exotic sheep breeds in jordan using microsatellites markers |
| topic | genetic diversity Jordan microsatellites sheep breeds |
| url | http://www.veterinaryworld.org/Vol.11/June-2018/7.pdf |
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