Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children
Background: The serodiagnostic tests for tuberculosis (TB) present a high variability in terms of sensitivity and specificity. Data on patients with latent TB infection (LTBI) and children in high prevalence settings are still limited. The present study aimed to evaluate an in-house strip test f...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2015-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/5403/10989_CE(Ra)_F(Sh)_PF1(SNAK)_PFA(AK)_PF2(PAG).pdf |
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| author | Songsri Kasempimolporn Wichit Thaveekarn Phirangkul Kerdpanich Urailak Skulpichetrat Orawan Saekhow Supatsorn Boonchang Thipchuta Bharnthong Visith Sitprija |
| author_facet | Songsri Kasempimolporn Wichit Thaveekarn Phirangkul Kerdpanich Urailak Skulpichetrat Orawan Saekhow Supatsorn Boonchang Thipchuta Bharnthong Visith Sitprija |
| author_sort | Songsri Kasempimolporn |
| collection | DOAJ |
| description | Background: The serodiagnostic tests for tuberculosis (TB)
present a high variability in terms of sensitivity and specificity.
Data on patients with latent TB infection (LTBI) and children
in high prevalence settings are still limited. The present study
aimed to evaluate an in-house strip test for detection of anti-M.
tuberculosis antibodies in TB patients, mostly children aged
under 15 y, grouped into four diagnostic categories: active TB,
LTBI, healthy TB contacts, and other non-TB diseases.
Materials and Methods: The diagnostic performance of
strip test was compared with the tuberculin skin test (TST)
and interferon-gamma release assay (IGRA). Sensitivity and
specificity were assessed for all three diagnostic tests. The
detection accuracy among the tests was calculated by using a
receiver operating characteristic analysis.
Results: TST and IGRA could diagnose the active TB cases
correctly (100%). The sensitivity of strip test for active TB was
58.3% and 37.5% for LTBI, while the sensitivities of TST and
IGRA for LTBI were 90.3% and 37.5%, respectively. The overall
specificities of strip test and IGRA were 91.5% and 95.7%,
respectively, which were superior to that of TST (68.1%).
Conclusion: The strip test did not appear to be useful for
diagnosis of active TB in comparison with the current diagnostic
standard. The assay may be particularly significant in situations
where TB is clinically difficult to diagnose like LTBI and could
be a meaningful tool in terms of high specificity and simplicity
for ruling in pediatric TB in countries with high TB infection rate.
Further studies are needed to determine whether strip test can
be improved in its sensitivity and should be implemented into
routine clinical practice. |
| first_indexed | 2024-12-10T19:03:26Z |
| format | Article |
| id | doaj.art-a0003bc5525b4ff68595ff6cb9a92c12 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-10T19:03:26Z |
| publishDate | 2015-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a0003bc5525b4ff68595ff6cb9a92c122022-12-22T01:36:57ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-01-0191DC11DC1410.7860/JCDR/2015/10989.5403Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in ChildrenSongsri Kasempimolporn0Wichit Thaveekarn1Phirangkul Kerdpanich2Urailak Skulpichetrat3Orawan Saekhow4Supatsorn Boonchang5Thipchuta Bharnthong6Visith Sitprija7Advisory Scientist, Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Scientist, Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Associate Professor, Infectious Disease Unit, Department of Pediatrics, Phramongkutklao Hospital, Bangkok, Thailand.Pediatric Fellow, Infectious Disease Unit, Department of Pediatrics, Phramongkutklao Hospital, Bangkok, Thailand.Senior Scientist, Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Laboratory Officer, Department of Research and Development, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Former Head, Department of BCG Vaccine Production, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Director Professor, Queen Saovabha Memorial Institute, Thai Red Cross Society, Bangkok, Thailand.Background: The serodiagnostic tests for tuberculosis (TB) present a high variability in terms of sensitivity and specificity. Data on patients with latent TB infection (LTBI) and children in high prevalence settings are still limited. The present study aimed to evaluate an in-house strip test for detection of anti-M. tuberculosis antibodies in TB patients, mostly children aged under 15 y, grouped into four diagnostic categories: active TB, LTBI, healthy TB contacts, and other non-TB diseases. Materials and Methods: The diagnostic performance of strip test was compared with the tuberculin skin test (TST) and interferon-gamma release assay (IGRA). Sensitivity and specificity were assessed for all three diagnostic tests. The detection accuracy among the tests was calculated by using a receiver operating characteristic analysis. Results: TST and IGRA could diagnose the active TB cases correctly (100%). The sensitivity of strip test for active TB was 58.3% and 37.5% for LTBI, while the sensitivities of TST and IGRA for LTBI were 90.3% and 37.5%, respectively. The overall specificities of strip test and IGRA were 91.5% and 95.7%, respectively, which were superior to that of TST (68.1%). Conclusion: The strip test did not appear to be useful for diagnosis of active TB in comparison with the current diagnostic standard. The assay may be particularly significant in situations where TB is clinically difficult to diagnose like LTBI and could be a meaningful tool in terms of high specificity and simplicity for ruling in pediatric TB in countries with high TB infection rate. Further studies are needed to determine whether strip test can be improved in its sensitivity and should be implemented into routine clinical practice.https://jcdr.net/articles/PDF/5403/10989_CE(Ra)_F(Sh)_PF1(SNAK)_PFA(AK)_PF2(PAG).pdfimmunochromatographic testinterferon-gamma release assaylatent tuberculosisserodiagnosistuberculin skin test |
| spellingShingle | Songsri Kasempimolporn Wichit Thaveekarn Phirangkul Kerdpanich Urailak Skulpichetrat Orawan Saekhow Supatsorn Boonchang Thipchuta Bharnthong Visith Sitprija Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children Journal of Clinical and Diagnostic Research immunochromatographic test interferon-gamma release assay latent tuberculosis serodiagnosis tuberculin skin test |
| title | Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children |
| title_full | Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children |
| title_fullStr | Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children |
| title_full_unstemmed | Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children |
| title_short | Performance of a Rapid Strip Test for the Serologic Diagnosis of Latent Tuberculosis in Children |
| title_sort | performance of a rapid strip test for the serologic diagnosis of latent tuberculosis in children |
| topic | immunochromatographic test interferon-gamma release assay latent tuberculosis serodiagnosis tuberculin skin test |
| url | https://jcdr.net/articles/PDF/5403/10989_CE(Ra)_F(Sh)_PF1(SNAK)_PFA(AK)_PF2(PAG).pdf |
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