Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells
Abstract Background Toxoplasmosis during pregnancy is known for inducing variable serious outcomes. In many previous studies, pregnancy was evaluated as a single event while in reality; it has different distinct immunological stages depending on gestational time and possible external factors as infe...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-08-01
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| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s43042-019-0006-5 |
| _version_ | 1828516450483044352 |
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| author | Mona Said El-Sherbini Amany A. Abd El-Aal Walid Sayed El-Sherbiny Samar Sayed Attia Inas Z. Abdel Aziz Ghada M. Nasr Mohamed Sayed Salama Mohamed S. Badr |
| author_facet | Mona Said El-Sherbini Amany A. Abd El-Aal Walid Sayed El-Sherbiny Samar Sayed Attia Inas Z. Abdel Aziz Ghada M. Nasr Mohamed Sayed Salama Mohamed S. Badr |
| author_sort | Mona Said El-Sherbini |
| collection | DOAJ |
| description | Abstract Background Toxoplasmosis during pregnancy is known for inducing variable serious outcomes. In many previous studies, pregnancy was evaluated as a single event while in reality; it has different distinct immunological stages depending on gestational time and possible external factors as infectious agents. A state of immunological balance as well as a state of maternal-fetal tolerance should be established; otherwise, atypical unwelcomed outcomes might occur as a result of such infection Aim This work aimed to study the gene expression profile of some pro- and anti-inflammatory cytokines in Toxoplasma seropositive cases Results All studied cytokines were significantly upregulated in group I, with a remarkable bias towards IFN-γ, denoting a predominant inflammatory state in group I. In group II; tested cytokines were approximately in a balanced state with a little shift towards anti-inflammatory cytokines (IL-10 and TGF-β). A significant positive correlation was detected between pro-inflammatory together (p = 0.002, r = 0.96) and between anti-inflammatory cytokines together (p = 0.0008, r = 0.99) in group II, indicating the harmonized and synchronized expression of both pro- and anti-inflammatory cytokines. In group III, IL-10 was relatively higher with negative correlation with IFN-γ (p = 0.006, r = 0.93), indicating the possible role of IL10 in saving some of the pregnancies’ outcomes in group III. Conclusion The present study suggests a potential role of these cytokines in the upcoming prognostic or/and therapeutic concerns of toxoplasmosis among pregnant women. |
| first_indexed | 2024-12-11T18:28:08Z |
| format | Article |
| id | doaj.art-a0009c835e624277bb7eca7a65db358a |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-12-11T18:28:08Z |
| publishDate | 2019-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-a0009c835e624277bb7eca7a65db358a2022-12-22T00:55:01ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412019-08-0120111010.1186/s43042-019-0006-5Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cellsMona Said El-Sherbini0Amany A. Abd El-Aal1Walid Sayed El-Sherbiny2Samar Sayed Attia3Inas Z. Abdel Aziz4Ghada M. Nasr5Mohamed Sayed Salama6Mohamed S. Badr7Department of Medical Parasitology, Faculty of Medicine, Cairo UniversityDepartment of Medical Parasitology, Faculty of Medicine, Cairo UniversityDepartment of Obstetrics & Gynaecology, Faculty of Medicine, Cairo UniversityDepartment of Medical Parasitology, Faculty of Medicine, Cairo UniversityDepartment of Medical Parasitology, Faculty of Medicine, Cairo UniversityDepartment of Molecular Diagnostics, Genetic Engineering and Biotechnology Research Institute, University of Sadat CityFaculty of Science, Ain Shams UniversityDepartment of Molecular Biology, Medical Research Center, Faculty of Medicine, Ain Shams UniversityAbstract Background Toxoplasmosis during pregnancy is known for inducing variable serious outcomes. In many previous studies, pregnancy was evaluated as a single event while in reality; it has different distinct immunological stages depending on gestational time and possible external factors as infectious agents. A state of immunological balance as well as a state of maternal-fetal tolerance should be established; otherwise, atypical unwelcomed outcomes might occur as a result of such infection Aim This work aimed to study the gene expression profile of some pro- and anti-inflammatory cytokines in Toxoplasma seropositive cases Results All studied cytokines were significantly upregulated in group I, with a remarkable bias towards IFN-γ, denoting a predominant inflammatory state in group I. In group II; tested cytokines were approximately in a balanced state with a little shift towards anti-inflammatory cytokines (IL-10 and TGF-β). A significant positive correlation was detected between pro-inflammatory together (p = 0.002, r = 0.96) and between anti-inflammatory cytokines together (p = 0.0008, r = 0.99) in group II, indicating the harmonized and synchronized expression of both pro- and anti-inflammatory cytokines. In group III, IL-10 was relatively higher with negative correlation with IFN-γ (p = 0.006, r = 0.93), indicating the possible role of IL10 in saving some of the pregnancies’ outcomes in group III. Conclusion The present study suggests a potential role of these cytokines in the upcoming prognostic or/and therapeutic concerns of toxoplasmosis among pregnant women.http://link.springer.com/article/10.1186/s43042-019-0006-5Toxoplasmaq-PCRIFN-γTNF-αIL10TGF-β |
| spellingShingle | Mona Said El-Sherbini Amany A. Abd El-Aal Walid Sayed El-Sherbiny Samar Sayed Attia Inas Z. Abdel Aziz Ghada M. Nasr Mohamed Sayed Salama Mohamed S. Badr Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells Egyptian Journal of Medical Human Genetics Toxoplasma q-PCR IFN-γ TNF-α IL10 TGF-β |
| title | Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells |
| title_full | Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells |
| title_fullStr | Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells |
| title_full_unstemmed | Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells |
| title_short | Toxoplasmosis and abortion: pro- and anti-inflammatory cytokines gene expression of the host immune cells |
| title_sort | toxoplasmosis and abortion pro and anti inflammatory cytokines gene expression of the host immune cells |
| topic | Toxoplasma q-PCR IFN-γ TNF-α IL10 TGF-β |
| url | http://link.springer.com/article/10.1186/s43042-019-0006-5 |
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