Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i>
Fungi produce numerous secondary metabolites with intriguing biological properties for the health, industrial, and agricultural sectors. Herein, we report the high-yield isolation of phenolic natural products, N-formyl-4-hydroxyphenyl-acetamide <b>1</b> (~117 mg/L) and atraric acid <b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/3/590 |
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| author | Harman Gill Ellen M. E. Sykes Ayush Kumar John L. Sorensen |
| author_facet | Harman Gill Ellen M. E. Sykes Ayush Kumar John L. Sorensen |
| author_sort | Harman Gill |
| collection | DOAJ |
| description | Fungi produce numerous secondary metabolites with intriguing biological properties for the health, industrial, and agricultural sectors. Herein, we report the high-yield isolation of phenolic natural products, N-formyl-4-hydroxyphenyl-acetamide <b>1</b> (~117 mg/L) and atraric acid <b>2</b> (~18 mg/L), from the ethyl acetate extract of the soil-derived fungus, <i>Aspergillus fumigatus</i>. The structures of compounds <b>1</b> and <b>2</b> were elucidated through the detailed spectroscopic analysis of NMR and LCMS data. These compounds were assayed for their antimicrobial activities. It was observed that compounds <b>1</b> and <b>2</b> exhibited strong inhibition against a series of fungal strains but only weak antibacterial properties against multi-drug-resistant strains. More significantly, this is the first known instance of the isolation of atraric acid <b>2</b> from a non-lichen fungal strain. We suggest the optimization of this fungal strain may exhibit elevated production of compounds <b>1</b> and <b>2</b>, potentially rendering it a valuable source for the industrial-scale production of these natural antimicrobial compounds. Further investigation is necessary to establish the veracity of this hypothesis. |
| first_indexed | 2024-03-09T11:00:23Z |
| format | Article |
| id | doaj.art-a005c4ab845642f3b2290cf57dcf14bc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T11:00:23Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a005c4ab845642f3b2290cf57dcf14bc2023-12-01T01:20:54ZengMDPI AGMicroorganisms2076-26072023-02-0111359010.3390/microorganisms11030590Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i>Harman Gill0Ellen M. E. Sykes1Ayush Kumar2John L. Sorensen3Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaFungi produce numerous secondary metabolites with intriguing biological properties for the health, industrial, and agricultural sectors. Herein, we report the high-yield isolation of phenolic natural products, N-formyl-4-hydroxyphenyl-acetamide <b>1</b> (~117 mg/L) and atraric acid <b>2</b> (~18 mg/L), from the ethyl acetate extract of the soil-derived fungus, <i>Aspergillus fumigatus</i>. The structures of compounds <b>1</b> and <b>2</b> were elucidated through the detailed spectroscopic analysis of NMR and LCMS data. These compounds were assayed for their antimicrobial activities. It was observed that compounds <b>1</b> and <b>2</b> exhibited strong inhibition against a series of fungal strains but only weak antibacterial properties against multi-drug-resistant strains. More significantly, this is the first known instance of the isolation of atraric acid <b>2</b> from a non-lichen fungal strain. We suggest the optimization of this fungal strain may exhibit elevated production of compounds <b>1</b> and <b>2</b>, potentially rendering it a valuable source for the industrial-scale production of these natural antimicrobial compounds. Further investigation is necessary to establish the veracity of this hypothesis.https://www.mdpi.com/2076-2607/11/3/590antibacterialN-formyl-4-hydroxyphenyl-acetamideatraric acidnon-lichen |
| spellingShingle | Harman Gill Ellen M. E. Sykes Ayush Kumar John L. Sorensen Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> Microorganisms antibacterial N-formyl-4-hydroxyphenyl-acetamide atraric acid non-lichen |
| title | Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> |
| title_full | Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> |
| title_fullStr | Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> |
| title_full_unstemmed | Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> |
| title_short | Isolation of Bioactive Metabolites from Soil Derived Fungus-<i>Aspergillus fumigatus</i> |
| title_sort | isolation of bioactive metabolites from soil derived fungus i aspergillus fumigatus i |
| topic | antibacterial N-formyl-4-hydroxyphenyl-acetamide atraric acid non-lichen |
| url | https://www.mdpi.com/2076-2607/11/3/590 |
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