Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea
<i>Helicobacter pylori</i> is the primary causative agent of gastritis, gastric ulcers, duodenal ulcers, gastric cancer, and peripheral B-cell lymphoma. <i>H. pylori</i> eradication often fails due to elevated antibiotic resistance. However, no previous studies have thoroughl...
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MDPI AG
2023-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/4/748 |
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| author | Soon Young Park Eun Hwa Lee Dokyun Kim Young Goo Song Su Jin Jeong |
| author_facet | Soon Young Park Eun Hwa Lee Dokyun Kim Young Goo Song Su Jin Jeong |
| author_sort | Soon Young Park |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> is the primary causative agent of gastritis, gastric ulcers, duodenal ulcers, gastric cancer, and peripheral B-cell lymphoma. <i>H. pylori</i> eradication often fails due to elevated antibiotic resistance. However, no previous studies have thoroughly examined amoxicillin resistance. Here, the objective was to identify clinical strains of <i>H. pylori</i> with amoxicillin resistance and to analyze single-nucleotide polymorphisms (SNPs) associated with amoxicillin resistance. From March 2015 to June 2019, genotypic and phenotypic amoxicillin resistance was analyzed using an E-test and whole-genome sequencing (WGS). Analysis of 368 clinical strains confirmed amoxicillin resistance in 31 strains (resistance rate of 8.7%). The genomes were extracted from nine resistant (<0.125 mg/L) strains, and WGS was performed for genetic analysis. WGS analysis identified SNPs present in <i>pbp1a</i>, <i>pbp2</i>, <i>nhaC</i>, <i>hofH</i>, <i>hofC</i>, and <i>hefC</i> in all nine isolates. Some of these genes may be related to amoxicillin resistance. A total of six SNPs (A69V, V374L, S414R, T503I, A592D, and R435Q) were identified in PBP2 of H-8, the most resistant strain. We predict that these six SNPs are associated with high amoxicillin resistance. Amoxicillin resistance should be considered in the clinical setting for the treatment failure of <i>H. pylori</i> eradication. |
| first_indexed | 2024-03-11T05:18:10Z |
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| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T05:18:10Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a006450b55704941b10fd793a7230cea2023-11-17T18:03:27ZengMDPI AGAntibiotics2079-63822023-04-0112474810.3390/antibiotics12040748Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South KoreaSoon Young Park0Eun Hwa Lee1Dokyun Kim2Young Goo Song3Su Jin Jeong4Division of Infectious Diseases, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Republic of KoreaDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 03722, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, Republic of KoreaDepartment of Internal Medicine, Yonsei University College of Medicine, Seoul 03722, Republic of Korea<i>Helicobacter pylori</i> is the primary causative agent of gastritis, gastric ulcers, duodenal ulcers, gastric cancer, and peripheral B-cell lymphoma. <i>H. pylori</i> eradication often fails due to elevated antibiotic resistance. However, no previous studies have thoroughly examined amoxicillin resistance. Here, the objective was to identify clinical strains of <i>H. pylori</i> with amoxicillin resistance and to analyze single-nucleotide polymorphisms (SNPs) associated with amoxicillin resistance. From March 2015 to June 2019, genotypic and phenotypic amoxicillin resistance was analyzed using an E-test and whole-genome sequencing (WGS). Analysis of 368 clinical strains confirmed amoxicillin resistance in 31 strains (resistance rate of 8.7%). The genomes were extracted from nine resistant (<0.125 mg/L) strains, and WGS was performed for genetic analysis. WGS analysis identified SNPs present in <i>pbp1a</i>, <i>pbp2</i>, <i>nhaC</i>, <i>hofH</i>, <i>hofC</i>, and <i>hefC</i> in all nine isolates. Some of these genes may be related to amoxicillin resistance. A total of six SNPs (A69V, V374L, S414R, T503I, A592D, and R435Q) were identified in PBP2 of H-8, the most resistant strain. We predict that these six SNPs are associated with high amoxicillin resistance. Amoxicillin resistance should be considered in the clinical setting for the treatment failure of <i>H. pylori</i> eradication.https://www.mdpi.com/2079-6382/12/4/748<i>Helicobacter pylori</i>antimicrobial susceptibility testingwhole-genome sequencingsingle-nucleotide polymorphismamoxicillinclarithromycin |
| spellingShingle | Soon Young Park Eun Hwa Lee Dokyun Kim Young Goo Song Su Jin Jeong Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea Antibiotics <i>Helicobacter pylori</i> antimicrobial susceptibility testing whole-genome sequencing single-nucleotide polymorphism amoxicillin clarithromycin |
| title | Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea |
| title_full | Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea |
| title_fullStr | Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea |
| title_full_unstemmed | Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea |
| title_short | Novel Mutations Conferring Amoxicillin Resistance in <i>Helicobacter pylori</i> in South Korea |
| title_sort | novel mutations conferring amoxicillin resistance in i helicobacter pylori i in south korea |
| topic | <i>Helicobacter pylori</i> antimicrobial susceptibility testing whole-genome sequencing single-nucleotide polymorphism amoxicillin clarithromycin |
| url | https://www.mdpi.com/2079-6382/12/4/748 |
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