Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula
Over a 24-month surveillance, three Cronobacter strains, NC041, NC830, and NC1006, were isolated from 77 powder infant formulas (3.90%). No Cronobacter was detected in liquid milk. The prevalence of Cronobacter in the prefinal product and packaged final product was 3.70% and 4.35%, respectively. The...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2011-08-01
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| Series: | Czech Journal of Food Sciences |
| Subjects: | |
| Online Access: | https://cjfs.agriculturejournals.cz/artkey/cjf-201104-0012_isolation-of-cronobacter-spp-isolates-from-infant-formulas-and-their-survival-in-the-production-process-of-inf.php |
| _version_ | 1797899758758526976 |
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| author | Songzhe Fu Jianxin Gao Ying Liu Haiying Chen |
| author_facet | Songzhe Fu Jianxin Gao Ying Liu Haiying Chen |
| author_sort | Songzhe Fu |
| collection | DOAJ |
| description | Over a 24-month surveillance, three Cronobacter strains, NC041, NC830, and NC1006, were isolated from 77 powder infant formulas (3.90%). No Cronobacter was detected in liquid milk. The prevalence of Cronobacter in the prefinal product and packaged final product was 3.70% and 4.35%, respectively. The isolated Cronobacter strains were subjected to several lethal challenges including the pH, drying, disinfectant, and simulated infant formulas manufacturing process (SIFMP). The results indicated that they exhibited unusual resistance to the dry stress and disinfectant. In SIFMP, Cronobacter isolates were inoculated into three possible contamination entry points involving the stages prior to heating, drying, and filling, respectively. No Cronobacter could survive the heating. However, a high level (105 CFU/ml) of Cronobacter was detected in the samples after the inoculation at the drying point. Furthermore, the survival of Cronobacter was observed during the storage at 10°C after 7 days. The results support the hypothesis that Cronobacter contamination occurs at the stages of drying and filling, and highlight the need to improve the disinfecting measures in the manufacturing process. |
| first_indexed | 2024-04-10T08:35:05Z |
| format | Article |
| id | doaj.art-a00a2b2a50514a50a53c17cd3d0d4711 |
| institution | Directory Open Access Journal |
| issn | 1212-1800 1805-9317 |
| language | English |
| last_indexed | 2024-04-10T08:35:05Z |
| publishDate | 2011-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Food Sciences |
| spelling | doaj.art-a00a2b2a50514a50a53c17cd3d0d47112023-02-23T03:27:40ZengCzech Academy of Agricultural SciencesCzech Journal of Food Sciences1212-18001805-93172011-08-0129439139910.17221/255/2010-CJFScjf-201104-0012Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formulaSongzhe Fu0Jianxin Gao1Ying Liu2Haiying Chen3Nanchang Center for Disease Control and Prevention, Nanchang, P.R. ChinaNanchang Center for Disease Control and Prevention, Nanchang, P.R. ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, P.R. ChinaNanchang Center for Disease Control and Prevention, Nanchang, P.R. ChinaOver a 24-month surveillance, three Cronobacter strains, NC041, NC830, and NC1006, were isolated from 77 powder infant formulas (3.90%). No Cronobacter was detected in liquid milk. The prevalence of Cronobacter in the prefinal product and packaged final product was 3.70% and 4.35%, respectively. The isolated Cronobacter strains were subjected to several lethal challenges including the pH, drying, disinfectant, and simulated infant formulas manufacturing process (SIFMP). The results indicated that they exhibited unusual resistance to the dry stress and disinfectant. In SIFMP, Cronobacter isolates were inoculated into three possible contamination entry points involving the stages prior to heating, drying, and filling, respectively. No Cronobacter could survive the heating. However, a high level (105 CFU/ml) of Cronobacter was detected in the samples after the inoculation at the drying point. Furthermore, the survival of Cronobacter was observed during the storage at 10°C after 7 days. The results support the hypothesis that Cronobacter contamination occurs at the stages of drying and filling, and highlight the need to improve the disinfecting measures in the manufacturing process.https://cjfs.agriculturejournals.cz/artkey/cjf-201104-0012_isolation-of-cronobacter-spp-isolates-from-infant-formulas-and-their-survival-in-the-production-process-of-inf.phpcronobacterdry stresspowdered infant formula (pif)contamination sources |
| spellingShingle | Songzhe Fu Jianxin Gao Ying Liu Haiying Chen Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula Czech Journal of Food Sciences cronobacter dry stress powdered infant formula (pif) contamination sources |
| title | Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula |
| title_full | Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula |
| title_fullStr | Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula |
| title_full_unstemmed | Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula |
| title_short | Isolation of Cronobacter spp. isolates from infant formulas and their survival in the production process of infant formula |
| title_sort | isolation of cronobacter spp isolates from infant formulas and their survival in the production process of infant formula |
| topic | cronobacter dry stress powdered infant formula (pif) contamination sources |
| url | https://cjfs.agriculturejournals.cz/artkey/cjf-201104-0012_isolation-of-cronobacter-spp-isolates-from-infant-formulas-and-their-survival-in-the-production-process-of-inf.php |
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