Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4
Enterobacterial common antigen (ECA) is a conserved surface antigen characteristic for <i>Enterobacteriaceae</i>. It is consisting of trisaccharide repeating unit, →3)-α-<span style="font-variant: small-caps;">d</span>-Fuc<i>p</i>4NAc-(1→4)-β-<span st...
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MDPI AG
2020-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/17/6038 |
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| author | Anna Maciejewska Marta Kaszowska Wojciech Jachymek Czeslaw Lugowski Jolanta Lukasiewicz |
| author_facet | Anna Maciejewska Marta Kaszowska Wojciech Jachymek Czeslaw Lugowski Jolanta Lukasiewicz |
| author_sort | Anna Maciejewska |
| collection | DOAJ |
| description | Enterobacterial common antigen (ECA) is a conserved surface antigen characteristic for <i>Enterobacteriaceae</i>. It is consisting of trisaccharide repeating unit, →3)-α-<span style="font-variant: small-caps;">d</span>-Fuc<i>p</i>4NAc-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Man<i>p</i>NAcA-(1→4)-α-<span style="font-variant: small-caps;">d</span>-Glc<i>p</i>NAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECA<sub>PG</sub>) and cyclic ECA (ECA<sub>CYC</sub>). Lipopolysaccharide (LPS)-associated form (ECA<sub>LPS</sub>) has been proved to date only for rough <i>Shigella sonnei</i> phase II. Depending on the structure organization, ECA constitutes surface antigen (ECA<sub>PG</sub> and ECA<sub>LPS</sub>) or maintains the outer membrane permeability barrier (ECA<sub>CYC</sub>). The existence of LPS was hypothesized in the 1960–80s on the basis of serological observations. Only a few <i>Escherichia coli</i> strains (i.e., R1, R2, R3, R4, and K-12) have led to the generation of anti-ECA antibodies upon immunization, excluding ECA<sub>PG</sub> as an immunogen and conjecturing ECA<sub>LPS</sub> as the only immunogenic form. Here, we presented a structural survey of ECA<sub>LPS</sub> in <i>E. coli</i> R1, R2, R3, and R4 to correlate previous serological observations with the presence of ECA<sub>LPS</sub>. The low yields of ECA<sub>LPS</sub> were identified in the R1, R2, and R4 strains, where ECA occupied outer core residues of LPS that used to be substituted by O-specific polysaccharide in the case of smooth LPS. Previously published observations and hypotheses regarding the immunogenicity and biosynthesis of ECA<sub>LPS</sub> were discussed and correlated with presented herein structural data. |
| first_indexed | 2024-03-10T17:01:06Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T17:01:06Z |
| publishDate | 2020-08-01 |
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| spelling | doaj.art-a00cba9bc68240f2983279f1844a76ce2023-11-20T10:58:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012117603810.3390/ijms21176038Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4Anna Maciejewska0Marta Kaszowska1Wojciech Jachymek2Czeslaw Lugowski3Jolanta Lukasiewicz4Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, PolandLaboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, PolandLaboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, PolandLaboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, PolandLaboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, PolandEnterobacterial common antigen (ECA) is a conserved surface antigen characteristic for <i>Enterobacteriaceae</i>. It is consisting of trisaccharide repeating unit, →3)-α-<span style="font-variant: small-caps;">d</span>-Fuc<i>p</i>4NAc-(1→4)-β-<span style="font-variant: small-caps;">d</span>-Man<i>p</i>NAcA-(1→4)-α-<span style="font-variant: small-caps;">d</span>-Glc<i>p</i>NAc-(1→, where prevailing forms include ECA linked to phosphatidylglycerol (ECA<sub>PG</sub>) and cyclic ECA (ECA<sub>CYC</sub>). Lipopolysaccharide (LPS)-associated form (ECA<sub>LPS</sub>) has been proved to date only for rough <i>Shigella sonnei</i> phase II. Depending on the structure organization, ECA constitutes surface antigen (ECA<sub>PG</sub> and ECA<sub>LPS</sub>) or maintains the outer membrane permeability barrier (ECA<sub>CYC</sub>). The existence of LPS was hypothesized in the 1960–80s on the basis of serological observations. Only a few <i>Escherichia coli</i> strains (i.e., R1, R2, R3, R4, and K-12) have led to the generation of anti-ECA antibodies upon immunization, excluding ECA<sub>PG</sub> as an immunogen and conjecturing ECA<sub>LPS</sub> as the only immunogenic form. Here, we presented a structural survey of ECA<sub>LPS</sub> in <i>E. coli</i> R1, R2, R3, and R4 to correlate previous serological observations with the presence of ECA<sub>LPS</sub>. The low yields of ECA<sub>LPS</sub> were identified in the R1, R2, and R4 strains, where ECA occupied outer core residues of LPS that used to be substituted by O-specific polysaccharide in the case of smooth LPS. Previously published observations and hypotheses regarding the immunogenicity and biosynthesis of ECA<sub>LPS</sub> were discussed and correlated with presented herein structural data.https://www.mdpi.com/1422-0067/21/17/6038enterobacterial common antigenECAlipopolysaccharideLPSECA<sub>LPS</sub>endotoxin |
| spellingShingle | Anna Maciejewska Marta Kaszowska Wojciech Jachymek Czeslaw Lugowski Jolanta Lukasiewicz Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 International Journal of Molecular Sciences enterobacterial common antigen ECA lipopolysaccharide LPS ECA<sub>LPS</sub> endotoxin |
| title | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 |
| title_full | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 |
| title_fullStr | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 |
| title_full_unstemmed | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 |
| title_short | Lipopolysaccharide-Linked Enterobacterial Common Antigen (ECA<sub>LPS</sub>) Occurs in Rough Strains of <i>Escherichia coli</i> R1, R2, and R4 |
| title_sort | lipopolysaccharide linked enterobacterial common antigen eca sub lps sub occurs in rough strains of i escherichia coli i r1 r2 and r4 |
| topic | enterobacterial common antigen ECA lipopolysaccharide LPS ECA<sub>LPS</sub> endotoxin |
| url | https://www.mdpi.com/1422-0067/21/17/6038 |
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