Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population
Objective This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods Three TMRs fo...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2021-04-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ajas-20-0386.pdf |
| _version_ | 1818677423036694528 |
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| author | Michelle Miguel Lovelia Mamuad Sonny Ramos Min Jung Ku Chang Dae Jeong Seon Ho Kim Yong Il Cho Sang Suk Lee |
| author_facet | Michelle Miguel Lovelia Mamuad Sonny Ramos Min Jung Ku Chang Dae Jeong Seon Ho Kim Yong Il Cho Sang Suk Lee |
| author_sort | Michelle Miguel |
| collection | DOAJ |
| description | Objective This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods Three TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonia-nitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3×2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems. Results Changes in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets. Conclusion The use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment. |
| first_indexed | 2024-12-17T08:59:08Z |
| format | Article |
| id | doaj.art-a00f8788e1524becbfb863ddd2c0c5d6 |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-17T08:59:08Z |
| publishDate | 2021-04-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-a00f8788e1524becbfb863ddd2c0c5d62022-12-21T21:55:49ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352021-04-0134464265110.5713/ajas.20.038624588Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial populationMichelle Miguel0Lovelia Mamuad1Sonny Ramos2Min Jung Ku3Chang Dae Jeong4Seon Ho Kim5Yong Il Cho6Sang Suk Lee7 Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Livestock Research Institute, Jeonnam Agricultural Research and Extension Services, Gangjin 59213, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea Department of Animal Science and Technology, College of Bio-industry Science, Sunchon National University, Suncheon 57922, KoreaObjective This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods Three TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonia-nitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3×2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems. Results Changes in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets. Conclusion The use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment.http://www.animbiosci.org/upload/pdf/ajas-20-0386.pdfforageinoculantitalian ryegrass silagerumentotal mixed ration |
| spellingShingle | Michelle Miguel Lovelia Mamuad Sonny Ramos Min Jung Ku Chang Dae Jeong Seon Ho Kim Yong Il Cho Sang Suk Lee Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population Animal Bioscience forage inoculant italian ryegrass silage rumen total mixed ration |
| title | Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| title_full | Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| title_fullStr | Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| title_full_unstemmed | Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| title_short | Effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| title_sort | effects of using different roughages in the total mixed ration inoculated with or without coculture of and on rumen fermentation and microbial population |
| topic | forage inoculant italian ryegrass silage rumen total mixed ration |
| url | http://www.animbiosci.org/upload/pdf/ajas-20-0386.pdf |
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