Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar
The genus <i>Populus</i> is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling s...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/14/12/2385 |
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| author | Ziyue Wang Yijing Lei Guanqing Liu Yihang Ning Runxin Ni Tao Zhang Mengli Xi |
| author_facet | Ziyue Wang Yijing Lei Guanqing Liu Yihang Ning Runxin Ni Tao Zhang Mengli Xi |
| author_sort | Ziyue Wang |
| collection | DOAJ |
| description | The genus <i>Populus</i> is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling stage. Therefore, developing a technology that identifies the gender of poplar seedlings is crucial for controlling seed hairs. In this study, we developed an approach for the early gender identification of <i>Tacamahaca</i> and <i>Aigeiros</i> species based on the male-specific sequence in <i>Populus simonii</i>. The gender of <i>Tacamahaca</i> and <i>Aigeiros</i> species can be accurately identified by PCR. The sequencing results showed that the male-specific sequence was conserved in <i>P. simonii</i> and its F<sub>1</sub> progenies. Interestingly, there were three nucleobase differences between <i>Tacamahaca</i> and <i>Aigeiros</i> species. Sequence alignment showed that the male-specific sequence had not been assembled on the pseudochromosome. Subsequently, fluorescence in situ hybridization (FISH) was used to locate this specific sequence at the short arm end of chromosome 19 in male <i>P. simonii</i>. This study provides an efficient and convenient method for early gender determination of <i>Tacamahaca</i> and <i>Aigeiros</i> species and lays the groundwork for exploring key sex-determination genes. |
| first_indexed | 2024-03-08T20:45:55Z |
| format | Article |
| id | doaj.art-a010bf14fe234c52a3ab6c4b556ac9cf |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-08T20:45:55Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Forests |
| spelling | doaj.art-a010bf14fe234c52a3ab6c4b556ac9cf2023-12-22T14:09:35ZengMDPI AGForests1999-49072023-12-011412238510.3390/f14122385Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of PoplarZiyue Wang0Yijing Lei1Guanqing Liu2Yihang Ning3Runxin Ni4Tao Zhang5Mengli Xi6State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou 225009, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaThe genus <i>Populus</i> is composed of dioecious woody plants and adult females produce large numbers of seed hairs that can affect public health and pose a potential fire risk. However, it is difficult to distinguish between males and females based on their morphology at the seedling stage. Therefore, developing a technology that identifies the gender of poplar seedlings is crucial for controlling seed hairs. In this study, we developed an approach for the early gender identification of <i>Tacamahaca</i> and <i>Aigeiros</i> species based on the male-specific sequence in <i>Populus simonii</i>. The gender of <i>Tacamahaca</i> and <i>Aigeiros</i> species can be accurately identified by PCR. The sequencing results showed that the male-specific sequence was conserved in <i>P. simonii</i> and its F<sub>1</sub> progenies. Interestingly, there were three nucleobase differences between <i>Tacamahaca</i> and <i>Aigeiros</i> species. Sequence alignment showed that the male-specific sequence had not been assembled on the pseudochromosome. Subsequently, fluorescence in situ hybridization (FISH) was used to locate this specific sequence at the short arm end of chromosome 19 in male <i>P. simonii</i>. This study provides an efficient and convenient method for early gender determination of <i>Tacamahaca</i> and <i>Aigeiros</i> species and lays the groundwork for exploring key sex-determination genes.https://www.mdpi.com/1999-4907/14/12/2385<i>Populus</i>gender identificationPCRFISH |
| spellingShingle | Ziyue Wang Yijing Lei Guanqing Liu Yihang Ning Runxin Ni Tao Zhang Mengli Xi Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar Forests <i>Populus</i> gender identification PCR FISH |
| title | Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar |
| title_full | Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar |
| title_fullStr | Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar |
| title_full_unstemmed | Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar |
| title_short | Male-Specific Sequence in <i>Populus simonii</i> Provides Insights into Gender Determination of Poplar |
| title_sort | male specific sequence in i populus simonii i provides insights into gender determination of poplar |
| topic | <i>Populus</i> gender identification PCR FISH |
| url | https://www.mdpi.com/1999-4907/14/12/2385 |
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