Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity
Abstract Background Klebsiella pneumoniae is a pathogen that often infects patients in clinical practice. Due to its high virulent and drug resistance, infected patients are difficult to treat. In clinical practice, Klebsiella pneumoniae can infect patients' intestines, intestines, blood, etc.,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-12-01
|
| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-023-08766-z |
| _version_ | 1797377147316207616 |
|---|---|
| author | Xu Wang Ting Li Lu Zhou Fan Tu Xiaohong Rui Ze Xu Jun Liu Futao Cao |
| author_facet | Xu Wang Ting Li Lu Zhou Fan Tu Xiaohong Rui Ze Xu Jun Liu Futao Cao |
| author_sort | Xu Wang |
| collection | DOAJ |
| description | Abstract Background Klebsiella pneumoniae is a pathogen that often infects patients in clinical practice. Due to its high virulent and drug resistance, infected patients are difficult to treat. In clinical practice, Klebsiella pneumoniae can infect patients' intestines, intestines, blood, etc., causing pathological changes. However, there is relatively little information on the impact of Klebsiella pneumoniae on intestinal inflammation and microbial populations. Zebrafish is an excellent biomedical model that has been successfully applied to the virulence assessment of Klebsiella pneumoniae. Methods In this study, three clinically isolated representative strains of Klebsiella pneumoniae (high virulence non-resistant, high virulence resistant, and low virulence resistant) were used to infect zebrafish, and their effects on intestinal colonization, inflammation, pathology, and microbial diversity were tested. Results Enzyme-linked immunoassay (ELISA) showed that Klebsiella pneumoniae significantly increased levels of the cytokines interleukin-1α (Il-1α), interleukin-1β (Il-1β), and tumor necrosis factor-α (Tnf-α), which increased inflammatory symptoms. Hematoxylin eosin staining(H&S) showed that Klebsiella pneumoniae treatment caused intestinal lesions in zebrafish, in which KP1053 exposure significantly decreased the number of goblet cells, KP1195 caused epithelial dissolution and exfoliation. In addition, Klebsiella pneumoniae disturbed the composition of intestinal microbiota, and the Shannon index increased, which increased the number of harmful bacteria. Conclusions Klebsiella pneumoniae infection can lead to intestinal colonization, inflammation, pathological changes, and changes in microbial biodiversity. This study provides a reference for the intestinal pathology of clinical Klebsiella pneumoniae infection. |
| first_indexed | 2024-03-08T19:49:32Z |
| format | Article |
| id | doaj.art-a013bb82e91c4c16a801a8bdd06eb822 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-03-08T19:49:32Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-a013bb82e91c4c16a801a8bdd06eb8222023-12-24T12:10:38ZengBMCBMC Infectious Diseases1471-23342023-12-0123111010.1186/s12879-023-08766-zAdult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversityXu Wang0Ting Li1Lu Zhou2Fan Tu3Xiaohong Rui4Ze Xu5Jun Liu6Futao Cao7Department of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan UniversityDepartment of Laboratory Medicine, Wuxi No.8 People’s HospitalDepartment of Laboratory Medicine, Jiangsu Provincial Center for Disease Control and PreventionDepartment of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan UniversityDepartment of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan UniversityDepartment of Laboratory Medicine, Wuxi Center for Disease Control and PreventionDepartment of Laboratory Medicine, Affiliated Wuxi Fifth Hospital of Jiangnan UniversityEmergency Department, Jiangnan University Medical CenterAbstract Background Klebsiella pneumoniae is a pathogen that often infects patients in clinical practice. Due to its high virulent and drug resistance, infected patients are difficult to treat. In clinical practice, Klebsiella pneumoniae can infect patients' intestines, intestines, blood, etc., causing pathological changes. However, there is relatively little information on the impact of Klebsiella pneumoniae on intestinal inflammation and microbial populations. Zebrafish is an excellent biomedical model that has been successfully applied to the virulence assessment of Klebsiella pneumoniae. Methods In this study, three clinically isolated representative strains of Klebsiella pneumoniae (high virulence non-resistant, high virulence resistant, and low virulence resistant) were used to infect zebrafish, and their effects on intestinal colonization, inflammation, pathology, and microbial diversity were tested. Results Enzyme-linked immunoassay (ELISA) showed that Klebsiella pneumoniae significantly increased levels of the cytokines interleukin-1α (Il-1α), interleukin-1β (Il-1β), and tumor necrosis factor-α (Tnf-α), which increased inflammatory symptoms. Hematoxylin eosin staining(H&S) showed that Klebsiella pneumoniae treatment caused intestinal lesions in zebrafish, in which KP1053 exposure significantly decreased the number of goblet cells, KP1195 caused epithelial dissolution and exfoliation. In addition, Klebsiella pneumoniae disturbed the composition of intestinal microbiota, and the Shannon index increased, which increased the number of harmful bacteria. Conclusions Klebsiella pneumoniae infection can lead to intestinal colonization, inflammation, pathological changes, and changes in microbial biodiversity. This study provides a reference for the intestinal pathology of clinical Klebsiella pneumoniae infection.https://doi.org/10.1186/s12879-023-08766-zKlebsiella pneumoniaZebrafishIntestineInflammationMicrobial diversity |
| spellingShingle | Xu Wang Ting Li Lu Zhou Fan Tu Xiaohong Rui Ze Xu Jun Liu Futao Cao Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity BMC Infectious Diseases Klebsiella pneumonia Zebrafish Intestine Inflammation Microbial diversity |
| title | Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| title_full | Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| title_fullStr | Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| title_full_unstemmed | Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| title_short | Adult zebrafish infected by clinically isolated Klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| title_sort | adult zebrafish infected by clinically isolated klebsiella pneumoniae with different virulence showed increased intestinal inflammation and disturbed intestinal microbial biodiversity |
| topic | Klebsiella pneumonia Zebrafish Intestine Inflammation Microbial diversity |
| url | https://doi.org/10.1186/s12879-023-08766-z |
| work_keys_str_mv | AT xuwang adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT tingli adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT luzhou adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT fantu adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT xiaohongrui adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT zexu adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT junliu adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity AT futaocao adultzebrafishinfectedbyclinicallyisolatedklebsiellapneumoniaewithdifferentvirulenceshowedincreasedintestinalinflammationanddisturbedintestinalmicrobialbiodiversity |