EVALUATION OF DECELLULARISATION PROTOCOLS FOR DEVELOPING EXTRACELLULAR MATRIX BASED PORCINE TUNICA VAGINALIS SCAFFOLDS FOR TISSUE ENGINEERING APPLICATIONS
The relevance of biomaterials to successfully restore the function of lost tissues to improve human health is increasing. There is a need to develop new biocompatible, low-cost biomaterial with better strength that can perform the intended purpose. Porcine tunica vaginalis, - a readily available a...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
West Bengal Veterinary Alumni Association
2023-06-01
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| Series: | Exploratory Animal and Medical Research |
| Subjects: | |
| Online Access: | https://www.animalmedicalresearch.org/Vol.13_Issue-1_June_2023/EVALUATION%20%20OF%20%20DECELLULARISATION.pdf |
| Summary: | The relevance of biomaterials to successfully restore the function of lost tissues to improve human health is
increasing. There is a need to develop new biocompatible, low-cost biomaterial with better strength that can perform the intended
purpose. Porcine tunica vaginalis, - a readily available abattoir bio-waste from slaughterhouses and being an expansion of the
peritoneum into the scrotum, can be considered a candidate scaffold. A study was carried out to examine the in-vitro characteristics
of variably decellularised porcine tunica vaginalis. The native or non-decellularised tissue was maintained as a control group
(PTV). The varied protocols for decellularisation included (a) Trypsin-EDTA-Triton-X-100 treated tunica vaginalis (DTV1) (b)
Sodium deoxycholate (SDC) and DNase treated porcine tunica vaginalis (DTV2) and (c) a combined protocol (DTV3) for
decellularising porcine tunica vaginalis. The efficiency of decellularisation was evaluated by histological examination, DNA
quantification, and 4, 6- diamidino-2-phenylindole (DAPI) nuclear staining. The biomechanical properties of the materials were
examined by analyzing tensile strength, maximum load at the break, and Young’s modulus. Further, the surface and other
structural characteristics were analyzed using scanning electron microscopy and special staining techniques. The concentration
of DNA, nuclear staining, and surface characters was in an acceptable range after decellularization for all three scaffolds. All
decellularisation protocols reduced DNA concentration significantly compared to the non-decellularised control and retained
collagen and other extracellular matrices (ECM) components. However, the DTV3 scaffold revealed minimal nuclear remnants,
acceptable DNA concentration as well as good surface topography. Comparison of biomechanical characters of the decellularised
scaffolds with non-decellularised tissue evidenced better tensile strength and Young’s modulus for DTV3. This work highlights
the influence of different decellularisation protocols on the final scaffolds which could pave the way for developing a novel
scaffold that can address various clinical problems in the medical field. |
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| ISSN: | 2277-470X 2319-247X |