Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor
The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovari...
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MDPI AG
2020-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/7/1190 |
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| author | Anna J. Korzekwa Angelika M. Kotlarczyk Agata A. Szczepańska Martyna Grzyb Alicja Siergiej Izabela Wocławek-Potocka |
| author_facet | Anna J. Korzekwa Angelika M. Kotlarczyk Agata A. Szczepańska Martyna Grzyb Alicja Siergiej Izabela Wocławek-Potocka |
| author_sort | Anna J. Korzekwa |
| collection | DOAJ |
| description | The aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovaries collected post mortem on the 4th and 13th days of the estrous cycle and during pregnancy (<i>n</i> = 18). After oocyte maturation, frozen-thawed epididymal semen was used for IVF. Blastocyst quality, apoptotic potential by determining the mRNA expression of <i>BAX</i>, <i>BCL-2</i>, <i>OCT4</i>, <i>SOX2</i>, and placenta-specific 8 gene (<i>PLAC8</i>)<i>,</i> and antioxidative potential of blastocysts were evaluated by determining the mRNA expression of <i>CuSOD</i>, <i>MnSOD</i>, and <i>GPX</i> as well as the enzymatic activity of superoxide dismutase and reduced glutathione. The highest development rate of expanded blastocyst, mRNA expression of <i>BCL-2</i>, <i>OCT4</i>, <i>SOX2</i>, and <i>PLAC8</i> and mRNA expression and enzymatic activity of the antioxidative factors increased (<i>p</i> < 0.05) in blastocysts developed from the oocytes collected on the 4th day, compared to those developed from the oocytes collected on the 13th day of the cycle and during pregnancy. Our study indicates that the 4th day of the estrous cycle is the most effective period for oocyte collection for IVF and embryo development in hinds, considering quality parameters and antioxidative potential of the blastocysts. |
| first_indexed | 2024-03-10T18:29:52Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:29:52Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a01a1a8b8ced4c57a40edba69e40f00a2023-11-20T06:42:40ZengMDPI AGAnimals2076-26152020-07-01107119010.3390/ani10071190Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte DonorAnna J. Korzekwa0Angelika M. Kotlarczyk1Agata A. Szczepańska2Martyna Grzyb3Alicja Siergiej4Izabela Wocławek-Potocka5Department of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Biodiversity Protection, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IAR&FR PAS), Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Gamete and Embryo Biology, IAR&FR PAS, Tuwima 10 Str., 10-748 Olsztyn, PolandDepartment of Gamete and Embryo Biology, IAR&FR PAS, Tuwima 10 Str., 10-748 Olsztyn, PolandThe aim was to compare the blastocyst stages of red deer embryos in respect of in vitro fertilization (IVF) efficiency, morphology, apoptotic and proliferative abilities, and antioxidative potential according to the reproductive status of hinds. We used three experimental groups, including the ovaries collected post mortem on the 4th and 13th days of the estrous cycle and during pregnancy (<i>n</i> = 18). After oocyte maturation, frozen-thawed epididymal semen was used for IVF. Blastocyst quality, apoptotic potential by determining the mRNA expression of <i>BAX</i>, <i>BCL-2</i>, <i>OCT4</i>, <i>SOX2</i>, and placenta-specific 8 gene (<i>PLAC8</i>)<i>,</i> and antioxidative potential of blastocysts were evaluated by determining the mRNA expression of <i>CuSOD</i>, <i>MnSOD</i>, and <i>GPX</i> as well as the enzymatic activity of superoxide dismutase and reduced glutathione. The highest development rate of expanded blastocyst, mRNA expression of <i>BCL-2</i>, <i>OCT4</i>, <i>SOX2</i>, and <i>PLAC8</i> and mRNA expression and enzymatic activity of the antioxidative factors increased (<i>p</i> < 0.05) in blastocysts developed from the oocytes collected on the 4th day, compared to those developed from the oocytes collected on the 13th day of the cycle and during pregnancy. Our study indicates that the 4th day of the estrous cycle is the most effective period for oocyte collection for IVF and embryo development in hinds, considering quality parameters and antioxidative potential of the blastocysts.https://www.mdpi.com/2076-2615/10/7/1190oxidative potentialblastocystembryored deer |
| spellingShingle | Anna J. Korzekwa Angelika M. Kotlarczyk Agata A. Szczepańska Martyna Grzyb Alicja Siergiej Izabela Wocławek-Potocka Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor Animals oxidative potential blastocyst embryo red deer |
| title | Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor |
| title_full | Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor |
| title_fullStr | Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor |
| title_full_unstemmed | Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor |
| title_short | Antioxidative Potential of Red Deer Embryos Depends on Reproductive Stage of Hind as a Oocyte Donor |
| title_sort | antioxidative potential of red deer embryos depends on reproductive stage of hind as a oocyte donor |
| topic | oxidative potential blastocyst embryo red deer |
| url | https://www.mdpi.com/2076-2615/10/7/1190 |
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