Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis
Salmonella is one of the major foodborne pathogens worldwide. The antimicrobial resistance (AMR) of this foodborne pathogen has raised a great concern in recent years. Studies on the frequency and characterization of Salmonella serotypes can help to improve our knowledge on the epidemiology of this...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2018-08-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/5482.pdf |
| _version_ | 1797425692091088896 |
|---|---|
| author | Mauricio Realpe-Quintero Jeannette Barba-León Julia A. Pérez-Montaño Carlos Pacheco-Gallardo Delia González-Aguilar Rosa M. Dominguez-Arias Elisa Cabrera-Diaz |
| author_facet | Mauricio Realpe-Quintero Jeannette Barba-León Julia A. Pérez-Montaño Carlos Pacheco-Gallardo Delia González-Aguilar Rosa M. Dominguez-Arias Elisa Cabrera-Diaz |
| author_sort | Mauricio Realpe-Quintero |
| collection | DOAJ |
| description | Salmonella is one of the major foodborne pathogens worldwide. The antimicrobial resistance (AMR) of this foodborne pathogen has raised a great concern in recent years. Studies on the frequency and characterization of Salmonella serotypes can help to improve our knowledge on the epidemiology of this pathogen. The purpose of this study was to compare the serotypes, AMR and genetic profiles of Salmonella isolates recovered from raw beef throughout the beef production chain and from human feces associated with clinical cases of salmonellosis. The serotype, AMR and pulsed-field gel electrophoresis profile of 243 Salmonella enterica isolates recovered from beef carcasses (n = 78), ground beef (n = 135), and human feces from clinical cases of salmonellosis (n = 30) were compared. Forty-three different Salmonella serotypes were identified and regardless of the source, the top five corresponded to Typhimurium, Give, Group B (partially serotyped), Infantis and Anatum. Twelve serotypes from beef carcasses were also found in ground beef, showing their presence throughout the beef production chain. Salmonella Typhimurium, Infantis, Anatum and Montevideo were the only serotypes identified in all sample types. Resistance to tetracyclines was the most frequent (41.2%) followed by resistance to aminoglycosides (37%), folate pathway inhibitors (21%), quinolones (20.2%), phenicols (17.1%), penicillins (15.6%) and cephems (7%). Multidrug resistance was observed in 28.8% of the isolates, and those from human feces showed resistance to a larger number of antimicrobials. Great concern arises from the resistance and reduced susceptibility observed to quinolones and cephalosporins because these drugs are the first line of treatment for invasive Salmonella infections. Twenty-seven distinct pulse-types were detected among 238 isolates. Clustering analysis for the most frequent serotypes identified groups of isolates with similar AMR profiles. Multidrug resistance spreading throughout the food production chain should be continually monitored and its importance emphasized. |
| first_indexed | 2024-03-09T08:19:50Z |
| format | Article |
| id | doaj.art-a01bd08cb5d94b639baaa563c032fc01 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T08:19:50Z |
| publishDate | 2018-08-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-a01bd08cb5d94b639baaa563c032fc012023-12-02T21:53:21ZengPeerJ Inc.PeerJ2167-83592018-08-016e548210.7717/peerj.5482Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosisMauricio Realpe-Quintero0Jeannette Barba-León1Julia A. Pérez-Montaño2Carlos Pacheco-Gallardo3Delia González-Aguilar4Rosa M. Dominguez-Arias5Elisa Cabrera-Diaz6Universidad de Guadalajara, Departamento de Medicina Veterinaria, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoUniversidad de Guadalajara, Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoUniversidad de Guadalajara, Marcelino García Barragán, Departamento de Farmacobiología, Centro Universitario de Ciencias Exactas e Ingenierías, Guadalajara, Jalisco, MexicoUniversidad de Guadalajara, Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoUniversidad de Guadalajara, Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoUniversidad de Guadalajara, Departamento de Biología Celular y Molecular, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoUniversidad de Guadalajara, Departamento de Salud Pública, Centro Universitario de Ciencias Biológicas y Agropecuarias, Zapopan, Jalisco, MexicoSalmonella is one of the major foodborne pathogens worldwide. The antimicrobial resistance (AMR) of this foodborne pathogen has raised a great concern in recent years. Studies on the frequency and characterization of Salmonella serotypes can help to improve our knowledge on the epidemiology of this pathogen. The purpose of this study was to compare the serotypes, AMR and genetic profiles of Salmonella isolates recovered from raw beef throughout the beef production chain and from human feces associated with clinical cases of salmonellosis. The serotype, AMR and pulsed-field gel electrophoresis profile of 243 Salmonella enterica isolates recovered from beef carcasses (n = 78), ground beef (n = 135), and human feces from clinical cases of salmonellosis (n = 30) were compared. Forty-three different Salmonella serotypes were identified and regardless of the source, the top five corresponded to Typhimurium, Give, Group B (partially serotyped), Infantis and Anatum. Twelve serotypes from beef carcasses were also found in ground beef, showing their presence throughout the beef production chain. Salmonella Typhimurium, Infantis, Anatum and Montevideo were the only serotypes identified in all sample types. Resistance to tetracyclines was the most frequent (41.2%) followed by resistance to aminoglycosides (37%), folate pathway inhibitors (21%), quinolones (20.2%), phenicols (17.1%), penicillins (15.6%) and cephems (7%). Multidrug resistance was observed in 28.8% of the isolates, and those from human feces showed resistance to a larger number of antimicrobials. Great concern arises from the resistance and reduced susceptibility observed to quinolones and cephalosporins because these drugs are the first line of treatment for invasive Salmonella infections. Twenty-seven distinct pulse-types were detected among 238 isolates. Clustering analysis for the most frequent serotypes identified groups of isolates with similar AMR profiles. Multidrug resistance spreading throughout the food production chain should be continually monitored and its importance emphasized.https://peerj.com/articles/5482.pdfAntimicrobial resistanceSerotypesPFGESalmonellaRaw beef |
| spellingShingle | Mauricio Realpe-Quintero Jeannette Barba-León Julia A. Pérez-Montaño Carlos Pacheco-Gallardo Delia González-Aguilar Rosa M. Dominguez-Arias Elisa Cabrera-Diaz Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis PeerJ Antimicrobial resistance Serotypes PFGE Salmonella Raw beef |
| title | Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| title_full | Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| title_fullStr | Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| title_full_unstemmed | Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| title_short | Genetic diversity and antimicrobial resistance of Salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| title_sort | genetic diversity and antimicrobial resistance of salmonella serotypes recovered throughout the beef production chain and from patients with salmonellosis |
| topic | Antimicrobial resistance Serotypes PFGE Salmonella Raw beef |
| url | https://peerj.com/articles/5482.pdf |
| work_keys_str_mv | AT mauriciorealpequintero geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT jeannettebarbaleon geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT juliaaperezmontano geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT carlospachecogallardo geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT deliagonzalezaguilar geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT rosamdominguezarias geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis AT elisacabreradiaz geneticdiversityandantimicrobialresistanceofsalmonellaserotypesrecoveredthroughoutthebeefproductionchainandfrompatientswithsalmonellosis |