Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i>
Huanglongbing, a globally devastating citrus disease, is associated with <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) and is mainly transmitted by <i>Diaphorina citri</i>. Verification of the distribution and dynamics of <i>C</i>Las in <i&...
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/10/8997 |
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| author | Xiaoge Nian Shujie Wu Jiayun Li Yaru Luo Jielan He Shijian Tan Desen Wang Yijing Cen Yurong He |
| author_facet | Xiaoge Nian Shujie Wu Jiayun Li Yaru Luo Jielan He Shijian Tan Desen Wang Yijing Cen Yurong He |
| author_sort | Xiaoge Nian |
| collection | DOAJ |
| description | Huanglongbing, a globally devastating citrus disease, is associated with <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) and is mainly transmitted by <i>Diaphorina citri</i>. Verification of the distribution and dynamics of <i>C</i>Las in <i>D. citri</i> is critical to understanding <i>C</i>Las transmitted by vectors in nature. Here, the distribution and titers of <i>C</i>Las in different sexes and tissues of <i>D. citri</i> adults were investigated by fluorescence in-situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR). Results showed that <i>C</i>Las had widespread distribution in the brain, salivary glands, digestive system, and reproductive system of both females and males, indicating a systemic infection of <i>C</i>Las in <i>D. citri</i>. Moreover, <i>C</i>Las fluorescence intensity and titers were significantly increased in both the digestive system and the female reproductive system with development and there was a marked decreased in both the salivary glands and the male brain, but there was no significant change in the female brain or the male reproductive system. Furthermore, the distribution and dynamics of <i>C</i>Las in embryos and nymphs were investigated. <i>C</i>Las was observed in all laid eggs and subsequent first–second-instar nymphs, indicating that a high percentage of embryos and nymphs resulting from infected <i>D. citri</i> mothers were infected with CLas. |
| first_indexed | 2024-03-11T03:38:46Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:38:46Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a01c5dabc16c4327b2226ad66fe6af522023-11-18T01:45:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410899710.3390/ijms24108997Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i>Xiaoge Nian0Shujie Wu1Jiayun Li2Yaru Luo3Jielan He4Shijian Tan5Desen Wang6Yijing Cen7Yurong He8Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, ChinaHuanglongbing, a globally devastating citrus disease, is associated with <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las) and is mainly transmitted by <i>Diaphorina citri</i>. Verification of the distribution and dynamics of <i>C</i>Las in <i>D. citri</i> is critical to understanding <i>C</i>Las transmitted by vectors in nature. Here, the distribution and titers of <i>C</i>Las in different sexes and tissues of <i>D. citri</i> adults were investigated by fluorescence in-situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR). Results showed that <i>C</i>Las had widespread distribution in the brain, salivary glands, digestive system, and reproductive system of both females and males, indicating a systemic infection of <i>C</i>Las in <i>D. citri</i>. Moreover, <i>C</i>Las fluorescence intensity and titers were significantly increased in both the digestive system and the female reproductive system with development and there was a marked decreased in both the salivary glands and the male brain, but there was no significant change in the female brain or the male reproductive system. Furthermore, the distribution and dynamics of <i>C</i>Las in embryos and nymphs were investigated. <i>C</i>Las was observed in all laid eggs and subsequent first–second-instar nymphs, indicating that a high percentage of embryos and nymphs resulting from infected <i>D. citri</i> mothers were infected with CLas.https://www.mdpi.com/1422-0067/24/10/8997<i>C</i>Las<i>D. citri</i>distributiondynamic |
| spellingShingle | Xiaoge Nian Shujie Wu Jiayun Li Yaru Luo Jielan He Shijian Tan Desen Wang Yijing Cen Yurong He Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> International Journal of Molecular Sciences <i>C</i>Las <i>D. citri</i> distribution dynamic |
| title | Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> |
| title_full | Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> |
| title_fullStr | Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> |
| title_full_unstemmed | Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> |
| title_short | Spatial Distribution and Temporal Dynamics of <i>Candidatus</i> Liberibacter Asiaticus in Different Stages of Embryos, Nymphs and Adults of <i>Diaphorina citri</i> |
| title_sort | spatial distribution and temporal dynamics of i candidatus i liberibacter asiaticus in different stages of embryos nymphs and adults of i diaphorina citri i |
| topic | <i>C</i>Las <i>D. citri</i> distribution dynamic |
| url | https://www.mdpi.com/1422-0067/24/10/8997 |
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