Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients
The IL-23/IL-17 axis plays causative roles in the development and progression of systemic lupus erythematosus (SLE). However, it remains unclear if the IL-17RA+ and IL-23R+ T helper (Th) cells populations are associated with the serum IL-17 and IL-23 levels, or with the immunological parameters and...
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| Format: | Article |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2021.690908/full |
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| author | Aziz Farah Izati Aziz Farah Izati Nur Diyana Mohd Shukri Nur Diyana Mohd Shukri Wan Syamimee Wan Ghazali Wan Syamimee Wan Ghazali Che Maraina Che Hussin Che Maraina Che Hussin Kah Keng Wong Kah Keng Wong |
| author_facet | Aziz Farah Izati Aziz Farah Izati Nur Diyana Mohd Shukri Nur Diyana Mohd Shukri Wan Syamimee Wan Ghazali Wan Syamimee Wan Ghazali Che Maraina Che Hussin Che Maraina Che Hussin Kah Keng Wong Kah Keng Wong |
| author_sort | Aziz Farah Izati |
| collection | DOAJ |
| description | The IL-23/IL-17 axis plays causative roles in the development and progression of systemic lupus erythematosus (SLE). However, it remains unclear if the IL-17RA+ and IL-23R+ T helper (Th) cells populations are associated with the serum IL-17 and IL-23 levels, or with the immunological parameters and disease activities in SLE patients. Herein, we examined the proportion of IL-17RA+ and IL-23R+ Th cells and serum levels of IL-17 and IL-23 in established SLE patients (n = 50) compared with healthy controls (n = 50). The associations of these interleukins and their receptors with immunological parameters [anti-nuclear antibody (ANA), anti-dsDNA antibody, and C-reactive protein (CRP)] and SLE disease activity (SLEDAI-2K scores) in SLE patients were assessed. CD3+CD4+ Th cells of SLE patients demonstrated significantly elevated IL-17RA+ (p = 1.12 x 10-4) or IL-23R+ (p = 1.98 x 10-29) populations compared with the healthy controls. Serum IL-17 levels were significantly lower in SLE patients compared with the healthy controls (p = 8.32 x 10-5), while no significant difference was observed for the IL-23 serum levels between both groups. IL-23R+ Th cells population was significantly associated with higher SLEDAI-2K scores (p = 0.017). In multivariate analysis, the proportion of IL-23R+ Th cells remained significantly associated with higher SLEDAI-2K scores independent of prednisolone intake (p = 0.027). No associations were observed between the interleukin parameters (i.e., IL-17, IL-23, IL-17RA+ Th cells, and IL-23R+ Th cells) with ANA, anti-dsDNA, and CRP status, suggesting that the IL-17/IL-23 axis acts independently of these immunological parameters. In conclusion, our results support that therapeutic inhibition of the IL-23/IL-17 axis receptors on Th cells, particularly IL-23R, is potentially relevant in SLE patients. |
| first_indexed | 2024-12-16T14:15:57Z |
| format | Article |
| id | doaj.art-a01d5a0a3a6b4a3dbbf938c8fe5b7660 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T14:15:57Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-a01d5a0a3a6b4a3dbbf938c8fe5b76602022-12-21T22:28:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-08-011210.3389/fimmu.2021.690908690908Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus PatientsAziz Farah Izati0Aziz Farah Izati1Nur Diyana Mohd Shukri2Nur Diyana Mohd Shukri3Wan Syamimee Wan Ghazali4Wan Syamimee Wan Ghazali5Che Maraina Che Hussin6Che Maraina Che Hussin7Kah Keng Wong8Kah Keng Wong9Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, MalaysiaHospital Universiti Sains Malaysia, Kubang Kerian, MalaysiaDepartment of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, MalaysiaHospital Universiti Sains Malaysia, Kubang Kerian, MalaysiaHospital Universiti Sains Malaysia, Kubang Kerian, MalaysiaDepartment of Internal Medicine, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, MalaysiaDepartment of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, MalaysiaHospital Universiti Sains Malaysia, Kubang Kerian, MalaysiaDepartment of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, MalaysiaHospital Universiti Sains Malaysia, Kubang Kerian, MalaysiaThe IL-23/IL-17 axis plays causative roles in the development and progression of systemic lupus erythematosus (SLE). However, it remains unclear if the IL-17RA+ and IL-23R+ T helper (Th) cells populations are associated with the serum IL-17 and IL-23 levels, or with the immunological parameters and disease activities in SLE patients. Herein, we examined the proportion of IL-17RA+ and IL-23R+ Th cells and serum levels of IL-17 and IL-23 in established SLE patients (n = 50) compared with healthy controls (n = 50). The associations of these interleukins and their receptors with immunological parameters [anti-nuclear antibody (ANA), anti-dsDNA antibody, and C-reactive protein (CRP)] and SLE disease activity (SLEDAI-2K scores) in SLE patients were assessed. CD3+CD4+ Th cells of SLE patients demonstrated significantly elevated IL-17RA+ (p = 1.12 x 10-4) or IL-23R+ (p = 1.98 x 10-29) populations compared with the healthy controls. Serum IL-17 levels were significantly lower in SLE patients compared with the healthy controls (p = 8.32 x 10-5), while no significant difference was observed for the IL-23 serum levels between both groups. IL-23R+ Th cells population was significantly associated with higher SLEDAI-2K scores (p = 0.017). In multivariate analysis, the proportion of IL-23R+ Th cells remained significantly associated with higher SLEDAI-2K scores independent of prednisolone intake (p = 0.027). No associations were observed between the interleukin parameters (i.e., IL-17, IL-23, IL-17RA+ Th cells, and IL-23R+ Th cells) with ANA, anti-dsDNA, and CRP status, suggesting that the IL-17/IL-23 axis acts independently of these immunological parameters. In conclusion, our results support that therapeutic inhibition of the IL-23/IL-17 axis receptors on Th cells, particularly IL-23R, is potentially relevant in SLE patients.https://www.frontiersin.org/articles/10.3389/fimmu.2021.690908/fullsystemic lupus erythematosusIL-23/IL-17 axisIL-17IL-23IL-17RAIL-23R |
| spellingShingle | Aziz Farah Izati Aziz Farah Izati Nur Diyana Mohd Shukri Nur Diyana Mohd Shukri Wan Syamimee Wan Ghazali Wan Syamimee Wan Ghazali Che Maraina Che Hussin Che Maraina Che Hussin Kah Keng Wong Kah Keng Wong Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients Frontiers in Immunology systemic lupus erythematosus IL-23/IL-17 axis IL-17 IL-23 IL-17RA IL-23R |
| title | Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients |
| title_full | Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients |
| title_fullStr | Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients |
| title_full_unstemmed | Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients |
| title_short | Increased IL-23R+ Th Cells Population Exhibits Higher SLEDAI-2K Scores in Systemic Lupus Erythematosus Patients |
| title_sort | increased il 23r th cells population exhibits higher sledai 2k scores in systemic lupus erythematosus patients |
| topic | systemic lupus erythematosus IL-23/IL-17 axis IL-17 IL-23 IL-17RA IL-23R |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2021.690908/full |
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