The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ
The small RNA ErsA of Pseudomonas aeruginosa was previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of the algC gene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00238/full |
| _version_ | 1811340554470948864 |
|---|---|
| author | Marilena Falcone Silvia Ferrara Elio Rossi Helle K. Johansen Helle K. Johansen Søren Molin Giovanni Bertoni |
| author_facet | Marilena Falcone Silvia Ferrara Elio Rossi Helle K. Johansen Helle K. Johansen Søren Molin Giovanni Bertoni |
| author_sort | Marilena Falcone |
| collection | DOAJ |
| description | The small RNA ErsA of Pseudomonas aeruginosa was previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of the algC gene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In this study, we show that a knock-out ersA mutant strain forms a flat and uniform biofilm, not characterized by mushroom-multicellular structures typical of a mature biofilm. Conversely, the knock-out mutant strain showed enhanced swarming and twitching motilities. To assess the influence of ErsA on the P. aeruginosa transcriptome, we performed RNA-seq experiments comparing the knock-out mutant with the wild-type. More than 160 genes were found differentially expressed in the knock-out mutant. Parts of these genes, important for biofilm formation and motility regulation, are known to belong also to the AmrZ transcriptional regulator regulon. Here, we show that ErsA binds in vitro and positively regulates amrZ mRNA at post-transcriptional level in vivo suggesting an interesting contribution of the ErsA-amrZ mRNA interaction in biofilm development at several regulatory levels. |
| first_indexed | 2024-04-13T18:43:42Z |
| format | Article |
| id | doaj.art-a020300641644c758fe3369a230435be |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T18:43:42Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a020300641644c758fe3369a230435be2022-12-22T02:34:39ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00238331577The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZMarilena Falcone0Silvia Ferrara1Elio Rossi2Helle K. Johansen3Helle K. Johansen4Søren Molin5Giovanni Bertoni6Dipartimento di Bioscienze, Università degli Studi di Milano, Milan, ItalyDipartimento di Bioscienze, Università degli Studi di Milano, Milan, ItalyDepartment of Clinical Microbiology, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Microbiology, Rigshospitalet, Copenhagen, DenmarkDepartment of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, DenmarkDipartimento di Bioscienze, Università degli Studi di Milano, Milan, ItalyThe small RNA ErsA of Pseudomonas aeruginosa was previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of the algC gene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In this study, we show that a knock-out ersA mutant strain forms a flat and uniform biofilm, not characterized by mushroom-multicellular structures typical of a mature biofilm. Conversely, the knock-out mutant strain showed enhanced swarming and twitching motilities. To assess the influence of ErsA on the P. aeruginosa transcriptome, we performed RNA-seq experiments comparing the knock-out mutant with the wild-type. More than 160 genes were found differentially expressed in the knock-out mutant. Parts of these genes, important for biofilm formation and motility regulation, are known to belong also to the AmrZ transcriptional regulator regulon. Here, we show that ErsA binds in vitro and positively regulates amrZ mRNA at post-transcriptional level in vivo suggesting an interesting contribution of the ErsA-amrZ mRNA interaction in biofilm development at several regulatory levels.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00238/fullPseudomonas aeruginosasmall regulatory RNApost-transcriptional regulationbiofilmvirulence |
| spellingShingle | Marilena Falcone Silvia Ferrara Elio Rossi Helle K. Johansen Helle K. Johansen Søren Molin Giovanni Bertoni The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ Frontiers in Microbiology Pseudomonas aeruginosa small regulatory RNA post-transcriptional regulation biofilm virulence |
| title | The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ |
| title_full | The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ |
| title_fullStr | The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ |
| title_full_unstemmed | The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ |
| title_short | The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ |
| title_sort | small rna ersa of pseudomonas aeruginosa contributes to biofilm development and motility through post transcriptional modulation of amrz |
| topic | Pseudomonas aeruginosa small regulatory RNA post-transcriptional regulation biofilm virulence |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00238/full |
| work_keys_str_mv | AT marilenafalcone thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT silviaferrara thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT eliorossi thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT hellekjohansen thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT hellekjohansen thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT sørenmolin thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT giovannibertoni thesmallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT marilenafalcone smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT silviaferrara smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT eliorossi smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT hellekjohansen smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT hellekjohansen smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT sørenmolin smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz AT giovannibertoni smallrnaersaofpseudomonasaeruginosacontributestobiofilmdevelopmentandmotilitythroughposttranscriptionalmodulationofamrz |