HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China
Objective To investigate the frequency and prognostic role of the human epidermal growth factor receptor 2 gene (HER2) and BRAF V600E gene mutation in Chinese patients with colorectal cancer (CRC). Methods Clinicopathological and survival information from 480 patients with stage I–III CRC were revie...
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| Format: | Article |
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PeerJ Inc.
2020-02-01
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| Online Access: | https://peerj.com/articles/8602.pdf |
| _version_ | 1797423854538194944 |
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| author | Xiangyan Zhang Jie Wu Lili Wang Han Zhao Hong Li Yuhe Duan Yujun Li Ping Xu Wenwen Ran Xiaoming Xing |
| author_facet | Xiangyan Zhang Jie Wu Lili Wang Han Zhao Hong Li Yuhe Duan Yujun Li Ping Xu Wenwen Ran Xiaoming Xing |
| author_sort | Xiangyan Zhang |
| collection | DOAJ |
| description | Objective To investigate the frequency and prognostic role of the human epidermal growth factor receptor 2 gene (HER2) and BRAF V600E gene mutation in Chinese patients with colorectal cancer (CRC). Methods Clinicopathological and survival information from 480 patients with stage I–III CRC were reviewed and recorded. HER2 amplification was analyzed by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), BRAF V600E mutation was tested by IHC and Sanger sequencing. The relationship between HER2 and BRAF V600E mutation status and clinicopathological characteristics and outcomes were determined. Results The amplification of HER2 and BRAF V600E mutation were identified in 27 of 480 (5.63%) and 19 of 480 (3.96%) CRC patients, respectively. HER2 amplification significantly correlated with greater bowel wall invasion (P = 0.041) and more advanced TNM stage (I vs. II vs. III; 0 vs 5.78% vs. 7.41%, P = 0.013). Patients suffering from tumors with poor differentiation had a higher incidence rate of BRAF V600E mutation than those with moderate/well differentiation (7.77% vs 2.92%, P = 0.04). HER2 amplification was an independent prognostic factor for worse disease-free survival (DFS) (HR = 2.53, 95% CI: 1.21–5.30, P = 0.014). Conclusion The prevalence of HER2 amplification and BRAF V600E mutation in stage I–III CRC patients in Chinese was 6% and 4%, respectively, and HER2 amplification appeared to be associated with a worse DFS. More comprehensive molecular classification and survival analysis are needed to validate our findings. |
| first_indexed | 2024-03-09T07:52:52Z |
| format | Article |
| id | doaj.art-a0223b296e284a74b0142bfcd394b08f |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:52:52Z |
| publishDate | 2020-02-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-a0223b296e284a74b0142bfcd394b08f2023-12-03T01:26:05ZengPeerJ Inc.PeerJ2167-83592020-02-018e860210.7717/peerj.8602HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern ChinaXiangyan Zhang0Jie Wu1Lili Wang2Han Zhao3Hong Li4Yuhe Duan5Yujun Li6Ping Xu7Wenwen Ran8Xiaoming Xing9Department of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pediatrics, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Obstetrics, Laixi People’s Hospital, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Pathology, Affiliated Hospital of Qingdao University, Qingdao, ChinaObjective To investigate the frequency and prognostic role of the human epidermal growth factor receptor 2 gene (HER2) and BRAF V600E gene mutation in Chinese patients with colorectal cancer (CRC). Methods Clinicopathological and survival information from 480 patients with stage I–III CRC were reviewed and recorded. HER2 amplification was analyzed by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH), BRAF V600E mutation was tested by IHC and Sanger sequencing. The relationship between HER2 and BRAF V600E mutation status and clinicopathological characteristics and outcomes were determined. Results The amplification of HER2 and BRAF V600E mutation were identified in 27 of 480 (5.63%) and 19 of 480 (3.96%) CRC patients, respectively. HER2 amplification significantly correlated with greater bowel wall invasion (P = 0.041) and more advanced TNM stage (I vs. II vs. III; 0 vs 5.78% vs. 7.41%, P = 0.013). Patients suffering from tumors with poor differentiation had a higher incidence rate of BRAF V600E mutation than those with moderate/well differentiation (7.77% vs 2.92%, P = 0.04). HER2 amplification was an independent prognostic factor for worse disease-free survival (DFS) (HR = 2.53, 95% CI: 1.21–5.30, P = 0.014). Conclusion The prevalence of HER2 amplification and BRAF V600E mutation in stage I–III CRC patients in Chinese was 6% and 4%, respectively, and HER2 amplification appeared to be associated with a worse DFS. More comprehensive molecular classification and survival analysis are needed to validate our findings.https://peerj.com/articles/8602.pdfHuman epidermal growth factor receptor 2 geneBRAF mutationColorectal cancerPrognosis |
| spellingShingle | Xiangyan Zhang Jie Wu Lili Wang Han Zhao Hong Li Yuhe Duan Yujun Li Ping Xu Wenwen Ran Xiaoming Xing HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China PeerJ Human epidermal growth factor receptor 2 gene BRAF mutation Colorectal cancer Prognosis |
| title | HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China |
| title_full | HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China |
| title_fullStr | HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China |
| title_full_unstemmed | HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China |
| title_short | HER2 and BRAF mutation in colorectal cancer patients: a retrospective study in Eastern China |
| title_sort | her2 and braf mutation in colorectal cancer patients a retrospective study in eastern china |
| topic | Human epidermal growth factor receptor 2 gene BRAF mutation Colorectal cancer Prognosis |
| url | https://peerj.com/articles/8602.pdf |
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