Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition
IntroductionNitrogen (N) deposition seriously affects the function of carbon (C) and N cycling in terrestrial ecosystems by altering soil microbial communities, especially in desert steppe ecosystems. However, there is a need for a comprehensive understanding of how microorganisms involved in each C...
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1369196/full |
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| author | He Ye He Ye Yu Zhao Yu Zhao Shilong He Shilong He Zhendan Wu Zhendan Wu Mei Yue Mei Yue Mei Hong Mei Hong |
| author_facet | He Ye He Ye Yu Zhao Yu Zhao Shilong He Shilong He Zhendan Wu Zhendan Wu Mei Yue Mei Yue Mei Hong Mei Hong |
| author_sort | He Ye |
| collection | DOAJ |
| description | IntroductionNitrogen (N) deposition seriously affects the function of carbon (C) and N cycling in terrestrial ecosystems by altering soil microbial communities, especially in desert steppe ecosystems. However, there is a need for a comprehensive understanding of how microorganisms involved in each C and N cycle process respond to N deposition.MethodsIn this study, shotgun metagenome sequencing was used to investigate variations in soil C and N cycling-related genes in the desert steppe in northern China after 6 years of the following N deposition: N0 (control); N30 (N addition 30 kg ha−1 year−1): N50 (N addition 50 kg ha−1 year−1).ResultsN deposition significantly increased the relative abundance of Actinobacteria (P < 0.05) while significantly decreased the relative abundances of Proteobacteria and Acidobacteria (P < 0.05). This significantly impacted the microbial community composition in desert steppe soils. The annual addition or deposition of 50 kg ha−1 year−1 for up to 6 years did not affect the C cycle gene abundance but changed the C cycle-related microorganism community structure. The process of the N cycle in the desert steppe was affected by N deposition (50 kg ha−1 year−1), which increased the abundance of the pmoA-amoA gene related to nitrification and the nirB gene associated with assimilation nitrite reductase. There may be a niche overlap between microorganisms involved in the same C and N cycling processes.DiscussionThis study provides new insights into the effects of N deposition on soil microbial communities and functions in desert steppe and a better understanding of the ecological consequences of anthropogenic N addition. |
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| language | English |
| last_indexed | 2024-04-24T19:17:38Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a022f773b3ea4936b02bdcbf2304f2112024-03-26T04:55:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13691961369196Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen depositionHe Ye0He Ye1Yu Zhao2Yu Zhao3Shilong He4Shilong He5Zhendan Wu6Zhendan Wu7Mei Yue8Mei Yue9Mei Hong10Mei Hong11Inner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaInner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaInner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaInner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaInner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaInner Mongolia Key Laboratory of Soil Quality and Nutrient Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, ChinaKey Laboratory of Agricultural Ecological Security and Green Development at Universities of Inner Mongolia Autonomous Region, Hohhot, ChinaIntroductionNitrogen (N) deposition seriously affects the function of carbon (C) and N cycling in terrestrial ecosystems by altering soil microbial communities, especially in desert steppe ecosystems. However, there is a need for a comprehensive understanding of how microorganisms involved in each C and N cycle process respond to N deposition.MethodsIn this study, shotgun metagenome sequencing was used to investigate variations in soil C and N cycling-related genes in the desert steppe in northern China after 6 years of the following N deposition: N0 (control); N30 (N addition 30 kg ha−1 year−1): N50 (N addition 50 kg ha−1 year−1).ResultsN deposition significantly increased the relative abundance of Actinobacteria (P < 0.05) while significantly decreased the relative abundances of Proteobacteria and Acidobacteria (P < 0.05). This significantly impacted the microbial community composition in desert steppe soils. The annual addition or deposition of 50 kg ha−1 year−1 for up to 6 years did not affect the C cycle gene abundance but changed the C cycle-related microorganism community structure. The process of the N cycle in the desert steppe was affected by N deposition (50 kg ha−1 year−1), which increased the abundance of the pmoA-amoA gene related to nitrification and the nirB gene associated with assimilation nitrite reductase. There may be a niche overlap between microorganisms involved in the same C and N cycling processes.DiscussionThis study provides new insights into the effects of N deposition on soil microbial communities and functions in desert steppe and a better understanding of the ecological consequences of anthropogenic N addition.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1369196/fullmetagenomicssoil microbiomecarbon cycle functionnitrogen cycle functionnitrogen deposition |
| spellingShingle | He Ye He Ye Yu Zhao Yu Zhao Shilong He Shilong He Zhendan Wu Zhendan Wu Mei Yue Mei Yue Mei Hong Mei Hong Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition Frontiers in Microbiology metagenomics soil microbiome carbon cycle function nitrogen cycle function nitrogen deposition |
| title | Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition |
| title_full | Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition |
| title_fullStr | Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition |
| title_full_unstemmed | Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition |
| title_short | Metagenomics reveals the response of desert steppe microbial communities and carbon-nitrogen cycling functional genes to nitrogen deposition |
| title_sort | metagenomics reveals the response of desert steppe microbial communities and carbon nitrogen cycling functional genes to nitrogen deposition |
| topic | metagenomics soil microbiome carbon cycle function nitrogen cycle function nitrogen deposition |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1369196/full |
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