Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes
Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly...
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KeAi Communications Co., Ltd.
2021-03-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014120300832 |
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| author | Wei Guo Zhuoqi Liu Jiaqi Yu Jing Lü Xiaoguo Jiao Lan Kong Chunxiao Yang Huipeng Pan |
| author_facet | Wei Guo Zhuoqi Liu Jiaqi Yu Jing Lü Xiaoguo Jiao Lan Kong Chunxiao Yang Huipeng Pan |
| author_sort | Wei Guo |
| collection | DOAJ |
| description | Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly generated 85 206 unigenes, with N50 of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and male H. vigintioctopunctata help explore genetic mechanisms of sexual dimorphism. |
| first_indexed | 2024-04-24T08:21:40Z |
| format | Article |
| id | doaj.art-a023f41f8c2e4755bd88d618f952fd40 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:21:40Z |
| publishDate | 2021-03-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Horticultural Plant Journal |
| spelling | doaj.art-a023f41f8c2e4755bd88d618f952fd402024-04-17T00:59:56ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412021-03-0172142148Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related GenesWei Guo0Zhuoqi Liu1Jiaqi Yu2Jing Lü3Xiaoguo Jiao4Lan Kong5Chunxiao Yang6Huipeng Pan7Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, South China Agricultural University, Guangzhou 510642, ChinaKey Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Biocatalysis and Enzyme Engineering, Center for Behavioral Ecology & Evolution, School of Life Sciences, Hubei University, Wuhan 430062, ChinaDepartment of Computer Science, Eastern Kentucky University, Richmond KY 40475, USAState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China; Corresponding authors. Phone: +86 15920435798Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, Engineering Technology Research Center of Agricultural Pest Biocontrol of Guangdong Province, South China Agricultural University, Guangzhou 510642, China; Corresponding authors. Phone: +86 15920435798Henosepilachna vigintioctopunctata is an emerging horticultural pest of Solanaceae plants in Asia. Here, we employed transcriptome sequencing of three female and three male H. vigintioctopunctata libraries to identify sex-related genes. We recorded 281 599 294 bp of clean reads, and de novo assembly generated 85 206 unigenes, with N50 of 1 311 bp and average length of 763 bp. We found 5 002 genes highly expressed in males and 2 179 genes highly expressed in females. Expression profiles of six unigenes specific to females, six unigenes specific to males, and ten unigenes common to both sexes were verified using semi-quantitive RT-PCR; results showed expression levels of these 22 unigenes were relatively consistent with the transcriptome analysis. Using all unigenes as references, we identified 6 657 putative simple sequence repeats. The transcriptome sequences and gene expression profiles of female and male H. vigintioctopunctata help explore genetic mechanisms of sexual dimorphism.http://www.sciencedirect.com/science/article/pii/S2468014120300832Henosepilachna vigintioctopunctatasexRNA-seqtranscriptome annotationdifferential expression |
| spellingShingle | Wei Guo Zhuoqi Liu Jiaqi Yu Jing Lü Xiaoguo Jiao Lan Kong Chunxiao Yang Huipeng Pan Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes Horticultural Plant Journal Henosepilachna vigintioctopunctata sex RNA-seq transcriptome annotation differential expression |
| title | Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes |
| title_full | Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes |
| title_fullStr | Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes |
| title_full_unstemmed | Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes |
| title_short | Transcriptome Analysis of Female and Male Henosepilachna vigintioctopunctata (Coleoptera: Coccinellidae) Identifies Sex-related Genes |
| title_sort | transcriptome analysis of female and male henosepilachna vigintioctopunctata coleoptera coccinellidae identifies sex related genes |
| topic | Henosepilachna vigintioctopunctata sex RNA-seq transcriptome annotation differential expression |
| url | http://www.sciencedirect.com/science/article/pii/S2468014120300832 |
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