Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits
<p class="MsoNormal" style="text-align: justify;">Amygdalin is a cyanogenic glycoside initially obtained from the seeds of bitter almonds. It is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid. Various ways of amygdali...
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| Format: | Article |
| Language: | English |
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HACCP Consulting
2017-01-01
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| Series: | Potravinarstvo |
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| Online Access: | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/756 |
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| author | Katarína Zbyňovská Marek Halenár Ľubica Chrastinová Ľubomír Ondruška Rastislav Jurčík Peter Čupka Eva Tušimová Anton Kováčik Eduard Kolesár Jozef Valuch Adriana Kolesarova |
| author_facet | Katarína Zbyňovská Marek Halenár Ľubica Chrastinová Ľubomír Ondruška Rastislav Jurčík Peter Čupka Eva Tušimová Anton Kováčik Eduard Kolesár Jozef Valuch Adriana Kolesarova |
| author_sort | Katarína Zbyňovská |
| collection | DOAJ |
| description | <p class="MsoNormal" style="text-align: justify;">Amygdalin is a cyanogenic glycoside initially obtained from the seeds of bitter almonds. It is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid. Various ways of amygdalin application play a different role in recipient organism. Intravenous infusion of amygdalin produced neither cyanidemia nor signs of toxicity, but oral administration resulted in significant blood cyanide levels. The present <em>in vivo</em> study was designed to reveal whether amygdalin is able to cause changes in the haematological profile and thus alter the physiological functions, using rabbits as a biological model. Adult male rabbits (n = 20) were randomly divided into five groups: the control group without any amygdalin administration, two experimental groups received a daily intramuscular injection of amygdalin at a dose 0.6 and 3.0 mg.kg<sup>-1</sup> b.w. and other two groups were fed by crushed apricot seeds at dose 60 and 300 mg. kg<sup>-1</sup> b.w., mixed with commercial feed over the period of 14 days. After two weeks, haematological parameters in whole blood were analysed (WBC - total white blood cell count, LYM - lymphocytes count, MID - medium size cell count, GRA - granulocytes count, RBC - red blood cell count, HGB - haemoglobin, HCT - haematocrit, MCV - mean corpuscular volume, MCH - mean corpuscular hemoglobin, MCHC - mean corpuscular hemoglobin concentration, RDWc - red cell distribution width, PLT - platelet count, PCT - platelet percentage, MPV - mean platelet volume, PDWc - platelet distribution width) using haematology analyser Abacus junior VET. Our findings indicate that intramuscular and oral application of amygdalin for two weeks did not significantly affect the haematology parameters in experimental animals. In this study, no obvious beneficial or negative effects of amygdalin administration on the blood of male rabbits were observed.</p> |
| first_indexed | 2024-12-18T06:37:58Z |
| format | Article |
| id | doaj.art-a024146168d54f77ac40ba8e5c730da3 |
| institution | Directory Open Access Journal |
| issn | 1337-0960 |
| language | English |
| last_indexed | 2024-12-18T06:37:58Z |
| publishDate | 2017-01-01 |
| publisher | HACCP Consulting |
| record_format | Article |
| series | Potravinarstvo |
| spelling | doaj.art-a024146168d54f77ac40ba8e5c730da32022-12-21T21:17:44ZengHACCP ConsultingPotravinarstvo1337-09602017-01-0111131532110.5219/756555Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbitsKatarína Zbyňovská0Marek Halenár1Ľubica Chrastinová2Ľubomír Ondruška3Rastislav Jurčík4Peter Čupka5Eva Tušimová6Anton Kováčik7Eduard Kolesár8Jozef Valuch9Adriana Kolesarova10Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraNational Agricultural and Food Center, Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 LužiankyNational Agricultural and Food Center, Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 LužiankyNational Agricultural and Food Center, Animal Production Research Centre Nitra, Hlohovecká 2, 951 41 LužiankySlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraResearch Centre AgroBioTech, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 NitraThe Health Care Surveillance Authority, Žellova 2, 829 24 Bratislava, tel.: +421-2-208 56 226Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Department of Animal Physiology, Tr. A. Hlinku 2, 949 76 Nitra<p class="MsoNormal" style="text-align: justify;">Amygdalin is a cyanogenic glycoside initially obtained from the seeds of bitter almonds. It is composed of one molecule of benzaldehyde, two molecules of glucose and one molecule of hydrocyanic acid. Various ways of amygdalin application play a different role in recipient organism. Intravenous infusion of amygdalin produced neither cyanidemia nor signs of toxicity, but oral administration resulted in significant blood cyanide levels. The present <em>in vivo</em> study was designed to reveal whether amygdalin is able to cause changes in the haematological profile and thus alter the physiological functions, using rabbits as a biological model. Adult male rabbits (n = 20) were randomly divided into five groups: the control group without any amygdalin administration, two experimental groups received a daily intramuscular injection of amygdalin at a dose 0.6 and 3.0 mg.kg<sup>-1</sup> b.w. and other two groups were fed by crushed apricot seeds at dose 60 and 300 mg. kg<sup>-1</sup> b.w., mixed with commercial feed over the period of 14 days. After two weeks, haematological parameters in whole blood were analysed (WBC - total white blood cell count, LYM - lymphocytes count, MID - medium size cell count, GRA - granulocytes count, RBC - red blood cell count, HGB - haemoglobin, HCT - haematocrit, MCV - mean corpuscular volume, MCH - mean corpuscular hemoglobin, MCHC - mean corpuscular hemoglobin concentration, RDWc - red cell distribution width, PLT - platelet count, PCT - platelet percentage, MPV - mean platelet volume, PDWc - platelet distribution width) using haematology analyser Abacus junior VET. Our findings indicate that intramuscular and oral application of amygdalin for two weeks did not significantly affect the haematology parameters in experimental animals. In this study, no obvious beneficial or negative effects of amygdalin administration on the blood of male rabbits were observed.</p>http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/756apricot seedamygdalinhaematologyrabbit |
| spellingShingle | Katarína Zbyňovská Marek Halenár Ľubica Chrastinová Ľubomír Ondruška Rastislav Jurčík Peter Čupka Eva Tušimová Anton Kováčik Eduard Kolesár Jozef Valuch Adriana Kolesarova Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits Potravinarstvo apricot seed amygdalin haematology rabbit |
| title | Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| title_full | Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| title_fullStr | Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| title_full_unstemmed | Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| title_short | Oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| title_sort | oral and intramuscular application of cyanogenic glycoside amygdalin did not induce changes in haematological profile of male rabbits |
| topic | apricot seed amygdalin haematology rabbit |
| url | http://www.potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/756 |
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