Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis
Electron shuttling molecules (ESMs) have been proven to accelerate the electron transfer from the electrode to the electroactive microorganism in microbial electrosynthesis (MES) for higher CO<sub>2</sub> reduction or chemical production rate. However, the microbial electron acceptors of...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/7/679 |
| _version_ | 1797589384315273216 |
|---|---|
| author | Jie Zhang He Liu Yan Zhang Bo Fu Chao Zhang Minhua Cui Ping Wu Chongjun Chen |
| author_facet | Jie Zhang He Liu Yan Zhang Bo Fu Chao Zhang Minhua Cui Ping Wu Chongjun Chen |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | Electron shuttling molecules (ESMs) have been proven to accelerate the electron transfer from the electrode to the electroactive microorganism in microbial electrosynthesis (MES) for higher CO<sub>2</sub> reduction or chemical production rate. However, the microbial electron acceptors of electroactive microorganisms and their responses to different electron shuttling molecules in MES were still unknown. In this study, three kinds of ESMs, e.g., riboflavin (B2), methyl viologen (MV) and neutral red (NR) were applied in the MES for acetate production to explore the mechanism of different ESMs on microbial interactions. The acetate concentrations were 41% and 51% higher than that of the control in B2 and NR addition. The acetogens relative abundances of control, B2, MV and NR were 0.29%, 5.68%, 22.78% and 42.89%, respectively. The microbial function profile of the microbial community on the biocathodes indicated that the performance of acetate production was more closely related to the expression of electron transport. The B2 was coupled with the NADH complex and hydrogenase, while MV and NR were coupled with the Rnf complex to support electron transfer and energy conversion via various electron transfer pathways. The study revealed that the ESMs coupled with different electron transport complexes of microorganisms to achieve electron transfer, resulting in product changes. |
| first_indexed | 2024-03-11T01:05:52Z |
| format | Article |
| id | doaj.art-a024876a89f34bf794c63f74e69e1a49 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:05:52Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-a024876a89f34bf794c63f74e69e1a492023-11-18T19:16:53ZengMDPI AGFermentation2311-56372023-07-019767910.3390/fermentation9070679Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial ElectrosynthesisJie Zhang0He Liu1Yan Zhang2Bo Fu3Chao Zhang4Minhua Cui5Ping Wu6Chongjun Chen7School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaSchool of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, ChinaJiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou 215009, ChinaElectron shuttling molecules (ESMs) have been proven to accelerate the electron transfer from the electrode to the electroactive microorganism in microbial electrosynthesis (MES) for higher CO<sub>2</sub> reduction or chemical production rate. However, the microbial electron acceptors of electroactive microorganisms and their responses to different electron shuttling molecules in MES were still unknown. In this study, three kinds of ESMs, e.g., riboflavin (B2), methyl viologen (MV) and neutral red (NR) were applied in the MES for acetate production to explore the mechanism of different ESMs on microbial interactions. The acetate concentrations were 41% and 51% higher than that of the control in B2 and NR addition. The acetogens relative abundances of control, B2, MV and NR were 0.29%, 5.68%, 22.78% and 42.89%, respectively. The microbial function profile of the microbial community on the biocathodes indicated that the performance of acetate production was more closely related to the expression of electron transport. The B2 was coupled with the NADH complex and hydrogenase, while MV and NR were coupled with the Rnf complex to support electron transfer and energy conversion via various electron transfer pathways. The study revealed that the ESMs coupled with different electron transport complexes of microorganisms to achieve electron transfer, resulting in product changes.https://www.mdpi.com/2311-5637/9/7/679microbial electrosynthesiselectron shuttling moleculesCO<sub>2</sub>acetate productionelectron transfer |
| spellingShingle | Jie Zhang He Liu Yan Zhang Bo Fu Chao Zhang Minhua Cui Ping Wu Chongjun Chen Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis Fermentation microbial electrosynthesis electron shuttling molecules CO<sub>2</sub> acetate production electron transfer |
| title | Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis |
| title_full | Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis |
| title_fullStr | Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis |
| title_full_unstemmed | Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis |
| title_short | Enhanced CO<sub>2</sub> Reduction by Electron Shuttle Molecules via Coupling Different Electron Transport Processes in Microbial Electrosynthesis |
| title_sort | enhanced co sub 2 sub reduction by electron shuttle molecules via coupling different electron transport processes in microbial electrosynthesis |
| topic | microbial electrosynthesis electron shuttling molecules CO<sub>2</sub> acetate production electron transfer |
| url | https://www.mdpi.com/2311-5637/9/7/679 |
| work_keys_str_mv | AT jiezhang enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT heliu enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT yanzhang enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT bofu enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT chaozhang enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT minhuacui enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT pingwu enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis AT chongjunchen enhancedcosub2subreductionbyelectronshuttlemoleculesviacouplingdifferentelectrontransportprocessesinmicrobialelectrosynthesis |