Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population
Background. Cytochrome P450 complex plays a key role in drug metabolism. CYP2B6 has an essential part in Cytochrome P450 complex metabolism. This study aims to determine the allelic distribution of CYP2B6∗2 and CYP2B6∗3 in three main Iranian ethnicities: Fars, Turk, and Kurd. Methods. The study was...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi - Cambridge University Press
2023-01-01
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| Series: | Genetics Research |
| Online Access: | http://dx.doi.org/10.1155/2023/8283470 |
| _version_ | 1797903045158240256 |
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| author | Armin Khavandegar Bahareh Tavakoli-Far Sarina Ansari Parisa Veis-Karami Faezeh Ghasemi Samira Sheibaninia Roshanak Jazayeri Massoud Houshmand |
| author_facet | Armin Khavandegar Bahareh Tavakoli-Far Sarina Ansari Parisa Veis-Karami Faezeh Ghasemi Samira Sheibaninia Roshanak Jazayeri Massoud Houshmand |
| author_sort | Armin Khavandegar |
| collection | DOAJ |
| description | Background. Cytochrome P450 complex plays a key role in drug metabolism. CYP2B6 has an essential part in Cytochrome P450 complex metabolism. This study aims to determine the allelic distribution of CYP2B6∗2 and CYP2B6∗3 in three main Iranian ethnicities: Fars, Turk, and Kurd. Methods. The study was conducted on 174 unrelated healthy volunteers from three main Iranian ethnicities. After DNA extraction from peripheral blood samples, genotyping of CYP2B6∗2 and ∗3 was performed using tetra ARMS and ARMS PCR, respectively. Results. The average age of 174 cases was 40.69 ± 11.87 (mean ± SD) and 39.06 ± 11.63 (mean ± SD) for males and females. In the CYP2B6∗2 variant, the genotyping frequency of wild type (C/C), heterozygous (C/T), and homozygous mutant (T/T) was 8.7%, 86%, and 5.2%, respectively. The CYP2B6∗2 (c.64C > T) allele frequency was 48.2% (95% CI: (37.8–58.6)). In the CYP2B6∗3 variant, the frequency of wild type (C/C), heterozygous (C/T), and homozygous mutant (T/T) was 75.3%, 11%, and 13.6%, respectively. The CYP2B6∗3 (c.777C > A) allelic frequency was 19.1% (95% CI: (17.5–20.7)). Conclusion. Allelic distribution in three main Iranian ethnicities, i.e., Turk, Kurd, and Fars, is remarkably higher than that in other populations, even that in Southern Iran. High frequencies of CYP2B6∗2 and ∗3 in the Iranian population highly affect drug responsiveness. Understanding such variability could help to increase drug efficacy and reduce its toxicity. |
| first_indexed | 2024-04-10T09:26:47Z |
| format | Article |
| id | doaj.art-a025e5783cba4963be8bd927d27fb1a5 |
| institution | Directory Open Access Journal |
| issn | 1469-5073 |
| language | English |
| last_indexed | 2024-04-10T09:26:47Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi - Cambridge University Press |
| record_format | Article |
| series | Genetics Research |
| spelling | doaj.art-a025e5783cba4963be8bd927d27fb1a52023-02-20T01:57:37ZengHindawi - Cambridge University PressGenetics Research1469-50732023-01-01202310.1155/2023/8283470Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian PopulationArmin Khavandegar0Bahareh Tavakoli-Far1Sarina Ansari2Parisa Veis-Karami3Faezeh Ghasemi4Samira Sheibaninia5Roshanak Jazayeri6Massoud Houshmand7Student Research CommitteeDietary Supplements and Probiotic Research CenterStudent Research CommitteeDepartment of Medical GeneticsDepartment of Medical GeneticsDepartment of Medical GeneticsDepartment of GeneticsDepartment of Medical GeneticsBackground. Cytochrome P450 complex plays a key role in drug metabolism. CYP2B6 has an essential part in Cytochrome P450 complex metabolism. This study aims to determine the allelic distribution of CYP2B6∗2 and CYP2B6∗3 in three main Iranian ethnicities: Fars, Turk, and Kurd. Methods. The study was conducted on 174 unrelated healthy volunteers from three main Iranian ethnicities. After DNA extraction from peripheral blood samples, genotyping of CYP2B6∗2 and ∗3 was performed using tetra ARMS and ARMS PCR, respectively. Results. The average age of 174 cases was 40.69 ± 11.87 (mean ± SD) and 39.06 ± 11.63 (mean ± SD) for males and females. In the CYP2B6∗2 variant, the genotyping frequency of wild type (C/C), heterozygous (C/T), and homozygous mutant (T/T) was 8.7%, 86%, and 5.2%, respectively. The CYP2B6∗2 (c.64C > T) allele frequency was 48.2% (95% CI: (37.8–58.6)). In the CYP2B6∗3 variant, the frequency of wild type (C/C), heterozygous (C/T), and homozygous mutant (T/T) was 75.3%, 11%, and 13.6%, respectively. The CYP2B6∗3 (c.777C > A) allelic frequency was 19.1% (95% CI: (17.5–20.7)). Conclusion. Allelic distribution in three main Iranian ethnicities, i.e., Turk, Kurd, and Fars, is remarkably higher than that in other populations, even that in Southern Iran. High frequencies of CYP2B6∗2 and ∗3 in the Iranian population highly affect drug responsiveness. Understanding such variability could help to increase drug efficacy and reduce its toxicity.http://dx.doi.org/10.1155/2023/8283470 |
| spellingShingle | Armin Khavandegar Bahareh Tavakoli-Far Sarina Ansari Parisa Veis-Karami Faezeh Ghasemi Samira Sheibaninia Roshanak Jazayeri Massoud Houshmand Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population Genetics Research |
| title | Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population |
| title_full | Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population |
| title_fullStr | Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population |
| title_full_unstemmed | Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population |
| title_short | Allelic and Genotype Frequencies of CYP2B6∗2 (64C > T) and CYP2B6∗3 (777C > A) in Three Dominant Ethnicities of the Iranian Population |
| title_sort | allelic and genotype frequencies of cyp2b6∗2 64c t and cyp2b6∗3 777c a in three dominant ethnicities of the iranian population |
| url | http://dx.doi.org/10.1155/2023/8283470 |
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