Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)
Abstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we r...
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Nature Portfolio
2017-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-02646-8 |
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| author | Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai |
| author_facet | Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai |
| author_sort | Songlin Li |
| collection | DOAJ |
| description | Abstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C18 and C20 PUFA, with only low activity towards C22 PUFA. In contrast, croaker Elovl4 could effectively convert both C20 and C22 PUFA to longer polyenoic products up to C34. n-3 LC-PUFA suppressed transcription of the two elongase genes, as well as srebp-1 and lxrα, major regulators of hepatic lipid metabolism. The results of dual-luciferase reporter assays and in vitro studies both indicated that the transcriptions of elovl5 and elovl4 elongases could be regulated by Lxrα. Moreover, Lxrα could mediate the transcription of elovl4 directly or indirectly through regulating the transcription of srebp-1. The above findings contribute further insight and understanding of the mechanisms regulating LC-PUFA biosynthesis in marine fish species. |
| first_indexed | 2024-12-20T20:54:59Z |
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| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:54:59Z |
| publishDate | 2017-05-01 |
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| spelling | doaj.art-a0279deb36744645a8117f86db8ecf972022-12-21T19:26:51ZengNature PortfolioScientific Reports2045-23222017-05-017111510.1038/s41598-017-02646-8Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea)Songlin Li0Óscar Monroig1Tianjiao Wang2Yuhui Yuan3Juan Carlos Navarro4Francisco Hontoria5Kai Liao6Douglas R. Tocher7Kangsen Mai8Wei Xu9Qinghui Ai10Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaInstitute of Aquaculture, School of Natural Sciences, University of StirlingKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaInstituto de Acuicultura Torre de la Sal (IATS-CSIC)Instituto de Acuicultura Torre de la Sal (IATS-CSIC)Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaInstitute of Aquaculture, School of Natural Sciences, University of StirlingKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of ChinaAbstract In the present study, two elongases, Elovl4 and Elovl5, were functionally characterized and their transcriptional regulation in response to n-3 LC-PUFA administration were investigated in vivo and in vitro. We previously described the molecular characterization of croaker elovl5. Here, we report the full-length cDNA sequence of croaker elovl4, which contained 1794 bp (excluding the polyA tail), including 909 bp of coding region that encoded a polypeptide of 302 amino acids possessing all the characteristic features of Elovl proteins. Functional studies showed that croaker Elovl5, displayed high elongation activity towards C18 and C20 PUFA, with only low activity towards C22 PUFA. In contrast, croaker Elovl4 could effectively convert both C20 and C22 PUFA to longer polyenoic products up to C34. n-3 LC-PUFA suppressed transcription of the two elongase genes, as well as srebp-1 and lxrα, major regulators of hepatic lipid metabolism. The results of dual-luciferase reporter assays and in vitro studies both indicated that the transcriptions of elovl5 and elovl4 elongases could be regulated by Lxrα. Moreover, Lxrα could mediate the transcription of elovl4 directly or indirectly through regulating the transcription of srebp-1. The above findings contribute further insight and understanding of the mechanisms regulating LC-PUFA biosynthesis in marine fish species.https://doi.org/10.1038/s41598-017-02646-8 |
| spellingShingle | Songlin Li Óscar Monroig Tianjiao Wang Yuhui Yuan Juan Carlos Navarro Francisco Hontoria Kai Liao Douglas R. Tocher Kangsen Mai Wei Xu Qinghui Ai Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) Scientific Reports |
| title | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
| title_full | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
| title_fullStr | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
| title_full_unstemmed | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
| title_short | Functional characterization and differential nutritional regulation of putative Elovl5 and Elovl4 elongases in large yellow croaker (Larimichthys crocea) |
| title_sort | functional characterization and differential nutritional regulation of putative elovl5 and elovl4 elongases in large yellow croaker larimichthys crocea |
| url | https://doi.org/10.1038/s41598-017-02646-8 |
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