Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS
Medicinal plant extracts are increasingly considered a major source of innovative medications and healthcare products. This study focused on preparing a polyphenol enriched water extract of Egyptian celery “<i>Apium graveolens</i> L., Apiaceae” aerial parts (TAE) in an endeavor to accent...
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MDPI AG
2022-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/3/698 |
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| author | Ayat M. Emad Dalia M. Rasheed Reham F. El-Kased Dina M. El-Kersh |
| author_facet | Ayat M. Emad Dalia M. Rasheed Reham F. El-Kased Dina M. El-Kersh |
| author_sort | Ayat M. Emad |
| collection | DOAJ |
| description | Medicinal plant extracts are increasingly considered a major source of innovative medications and healthcare products. This study focused on preparing a polyphenol enriched water extract of Egyptian celery “<i>Apium graveolens</i> L., Apiaceae” aerial parts (TAE) in an endeavor to accentuate its antioxidant capacity as well as its antimicrobial activity. (TAE) of celery was partitioned against different organic solvents to yield dichloromethane (DCM), ethyl acetate (EAC), and butanol (BUOH) fractions. (TAE) and the organic fractions thereof besides the remaining mother liquor (ML) were all screened for their antioxidant capacity using various protocols viz. monitoring the reducing amplitudes for ferric ions (FRAP), and radical scavenging potentials of oxygen (ORAC), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelation assays. The examination procedure revealed both (TAE) extract and (DCM) fraction, to pertain the highest antioxidant potentials, where the IC<sub>50</sub> of the (TAE) using ABTS and metal chelation assays were ca. 34.52 ± 3.25 and 246.6 ± 5.78 µg/mL, respectively. The (DCM) fraction recorded effective results using the FRAP, ORAC, and DPPH assays ca. 233.47 ± 15.14 and 1076 ± 25.73 μM Trolox equivalents/mg sample and an IC<sub>50</sub> 474.4 ± 19.8 µg/mL, respectively. Additionally, both (TAE) and (DCM) fraction exerted antimicrobial activities recording inhibition zones (mm) (13.4 ± 1.5) and (12.0 ± 1.0) against <i>Staphylococcus aureus</i> and (11.0 ± 1.2) and (10.0 ± 1.3) against <i>Escherichia coli</i>, respectively, with no anti-fungal activity. Minimum inhibitory concentration (MIC) of (TAE) and (DCM) fraction were 1250 and 2500 µg/mL, respectively. UPLC/ESI/TOF-MS unveiled the chemical profile of both (TAE) and (DCM) fraction to encompass a myriad of active polyphenolic constituents including phenylpropanoids, coumarins, apigenin, luteolin, and chrysoeriol conjugates. |
| first_indexed | 2024-03-09T23:30:25Z |
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| id | doaj.art-a02da44b3ed149cc9289c8e29c2e824d |
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| last_indexed | 2024-03-09T23:30:25Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-a02da44b3ed149cc9289c8e29c2e824d2023-11-23T17:11:02ZengMDPI AGMolecules1420-30492022-01-0127369810.3390/molecules27030698Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MSAyat M. Emad0Dalia M. Rasheed1Reham F. El-Kased2Dina M. El-Kersh3Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Sixth of October City 12511, EgyptPharmacognosy Department, Faculty of Pharmacy, October 6 University, Sixth of October City 12511, EgyptMicrobiology & Immunology Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, EgyptPharmacognosy Department, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, EgyptMedicinal plant extracts are increasingly considered a major source of innovative medications and healthcare products. This study focused on preparing a polyphenol enriched water extract of Egyptian celery “<i>Apium graveolens</i> L., Apiaceae” aerial parts (TAE) in an endeavor to accentuate its antioxidant capacity as well as its antimicrobial activity. (TAE) of celery was partitioned against different organic solvents to yield dichloromethane (DCM), ethyl acetate (EAC), and butanol (BUOH) fractions. (TAE) and the organic fractions thereof besides the remaining mother liquor (ML) were all screened for their antioxidant capacity using various protocols viz. monitoring the reducing amplitudes for ferric ions (FRAP), and radical scavenging potentials of oxygen (ORAC), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and metal chelation assays. The examination procedure revealed both (TAE) extract and (DCM) fraction, to pertain the highest antioxidant potentials, where the IC<sub>50</sub> of the (TAE) using ABTS and metal chelation assays were ca. 34.52 ± 3.25 and 246.6 ± 5.78 µg/mL, respectively. The (DCM) fraction recorded effective results using the FRAP, ORAC, and DPPH assays ca. 233.47 ± 15.14 and 1076 ± 25.73 μM Trolox equivalents/mg sample and an IC<sub>50</sub> 474.4 ± 19.8 µg/mL, respectively. Additionally, both (TAE) and (DCM) fraction exerted antimicrobial activities recording inhibition zones (mm) (13.4 ± 1.5) and (12.0 ± 1.0) against <i>Staphylococcus aureus</i> and (11.0 ± 1.2) and (10.0 ± 1.3) against <i>Escherichia coli</i>, respectively, with no anti-fungal activity. Minimum inhibitory concentration (MIC) of (TAE) and (DCM) fraction were 1250 and 2500 µg/mL, respectively. UPLC/ESI/TOF-MS unveiled the chemical profile of both (TAE) and (DCM) fraction to encompass a myriad of active polyphenolic constituents including phenylpropanoids, coumarins, apigenin, luteolin, and chrysoeriol conjugates.https://www.mdpi.com/1420-3049/27/3/698celery<i>Apium graveolens</i>antioxidantantimicrobialUPLC/ESI/TOF-MS |
| spellingShingle | Ayat M. Emad Dalia M. Rasheed Reham F. El-Kased Dina M. El-Kersh Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS Molecules celery <i>Apium graveolens</i> antioxidant antimicrobial UPLC/ESI/TOF-MS |
| title | Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS |
| title_full | Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS |
| title_fullStr | Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS |
| title_full_unstemmed | Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS |
| title_short | Antioxidant, Antimicrobial Activities and Characterization of Polyphenol-Enriched Extract of Egyptian Celery (<i>Apium graveolens</i> L., Apiaceae) Aerial Parts via UPLC/ESI/TOF-MS |
| title_sort | antioxidant antimicrobial activities and characterization of polyphenol enriched extract of egyptian celery i apium graveolens i l apiaceae aerial parts via uplc esi tof ms |
| topic | celery <i>Apium graveolens</i> antioxidant antimicrobial UPLC/ESI/TOF-MS |
| url | https://www.mdpi.com/1420-3049/27/3/698 |
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