Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns
Due to the relevance of cleaning and disinfection in farm hygiene management, accurate evaluation of the success of such procedures remains a fundamental challenge for producers. This study aimed to use boot swab sampling to quantify the effects of such practices in poultry barns. For this purpose,...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Agriculture |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0472/12/1/57 |
| _version_ | 1797496872217083904 |
|---|---|
| author | Rafael H. Mateus-Vargas Kira Butenholz Nina Volkmann Christian Sürie Nicole Kemper Jochen Schulz |
| author_facet | Rafael H. Mateus-Vargas Kira Butenholz Nina Volkmann Christian Sürie Nicole Kemper Jochen Schulz |
| author_sort | Rafael H. Mateus-Vargas |
| collection | DOAJ |
| description | Due to the relevance of cleaning and disinfection in farm hygiene management, accurate evaluation of the success of such procedures remains a fundamental challenge for producers. This study aimed to use boot swab sampling to quantify the effects of such practices in poultry barns. For this purpose, the counts of both the total and fecal indicator bacteria were detected after the application of a cleaning and disinfection protocol in identical barns that were occupied by turkeys and broilers. Boot swab samples were compared to an established agar contact plating method to evaluate disinfection success. Statistical analyses showed no correlations between the bacterial counts that were obtained with either method. In contrast to the agar contact plating method, boot swab sampling permitted the assessment of the hygienic conditions of the barn floors before and after cleaning procedures. Furthermore, according to observations with the boot swab method, factors related to the species being farmed influenced the initial bacterial loads but did not affect the effectiveness of cleaning and disinfection. Species identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) also suggested that non-fecal bacteria grow on selective media. Further studies should validate the use of this sampling technique by comparing different cleaning and disinfection protocols. |
| first_indexed | 2024-03-10T03:09:41Z |
| format | Article |
| id | doaj.art-a02f23fc02084128bedcf061fc4c2368 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-10T03:09:41Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-a02f23fc02084128bedcf061fc4c23682023-11-23T12:35:31ZengMDPI AGAgriculture2077-04722022-01-011215710.3390/agriculture12010057Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry BarnsRafael H. Mateus-Vargas0Kira Butenholz1Nina Volkmann2Christian Sürie3Nicole Kemper4Jochen Schulz5Institute for Animal Hygiene, Animal Welfare and Farm Animal Behavior (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), D-30173 Hannover, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behavior (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), D-30173 Hannover, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behavior (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), D-30173 Hannover, GermanyLehr-Und Forschungsgut Ruthe, University of Veterinary Medicine Hannover, Foundation, Schäferberg 1, D-31157 Sarstedt, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behavior (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), D-30173 Hannover, GermanyInstitute for Animal Hygiene, Animal Welfare and Farm Animal Behavior (ITTN), University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15 (Building 116), D-30173 Hannover, GermanyDue to the relevance of cleaning and disinfection in farm hygiene management, accurate evaluation of the success of such procedures remains a fundamental challenge for producers. This study aimed to use boot swab sampling to quantify the effects of such practices in poultry barns. For this purpose, the counts of both the total and fecal indicator bacteria were detected after the application of a cleaning and disinfection protocol in identical barns that were occupied by turkeys and broilers. Boot swab samples were compared to an established agar contact plating method to evaluate disinfection success. Statistical analyses showed no correlations between the bacterial counts that were obtained with either method. In contrast to the agar contact plating method, boot swab sampling permitted the assessment of the hygienic conditions of the barn floors before and after cleaning procedures. Furthermore, according to observations with the boot swab method, factors related to the species being farmed influenced the initial bacterial loads but did not affect the effectiveness of cleaning and disinfection. Species identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) also suggested that non-fecal bacteria grow on selective media. Further studies should validate the use of this sampling technique by comparing different cleaning and disinfection protocols.https://www.mdpi.com/2077-0472/12/1/57boot swab samplinghygiene evaluationtotal aerobic countsEnterococci |
| spellingShingle | Rafael H. Mateus-Vargas Kira Butenholz Nina Volkmann Christian Sürie Nicole Kemper Jochen Schulz Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns Agriculture boot swab sampling hygiene evaluation total aerobic counts Enterococci |
| title | Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns |
| title_full | Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns |
| title_fullStr | Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns |
| title_full_unstemmed | Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns |
| title_short | Boot Swabs to Evaluate Cleaning and Disinfection Success in Poultry Barns |
| title_sort | boot swabs to evaluate cleaning and disinfection success in poultry barns |
| topic | boot swab sampling hygiene evaluation total aerobic counts Enterococci |
| url | https://www.mdpi.com/2077-0472/12/1/57 |
| work_keys_str_mv | AT rafaelhmateusvargas bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns AT kirabutenholz bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns AT ninavolkmann bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns AT christiansurie bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns AT nicolekemper bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns AT jochenschulz bootswabstoevaluatecleaninganddisinfectionsuccessinpoultrybarns |