Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite
Objective To investigate the inhibition of house dust mite induced oxidative stress with human bronchial epithelial cell line BEAS-2B by aldose reductase inhibitor (ARIs). Methods BEAS-2B was divided into control group, model group, NF-κB inhibitor group (PDTC group) and ARIs intervention group (ARI...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-04-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-616.pdf |
| _version_ | 1797361448696938496 |
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| author | BAO Min, LIN Mei, ZHEN Hai-ning, HE Fang, DING Min, YUAN Zhu-qing, CHEN Ya-jun, XUE Xin-xin |
| author_facet | BAO Min, LIN Mei, ZHEN Hai-ning, HE Fang, DING Min, YUAN Zhu-qing, CHEN Ya-jun, XUE Xin-xin |
| author_sort | BAO Min, LIN Mei, ZHEN Hai-ning, HE Fang, DING Min, YUAN Zhu-qing, CHEN Ya-jun, XUE Xin-xin |
| collection | DOAJ |
| description | Objective To investigate the inhibition of house dust mite induced oxidative stress with human bronchial epithelial cell line BEAS-2B by aldose reductase inhibitor (ARIs). Methods BEAS-2B was divided into control group, model group, NF-κB inhibitor group (PDTC group) and ARIs intervention group (ARIs group). Oxidative stress reaction (MDA and ROS content, SOD activity) was detected by ELISA. The contents of IL-5, IL-13 and IFN-γ were measured by ELISA. MTT was used to detect cell viability. Cell apoptosis was detected by flow cytometry. The expressions of NF-κB, NF-κB P65 and P38 MAPK proteins were detected by Western blot. Results SOD activity, IFN-γ content and cell survival rate in model group were lower than those in control group (P＜0.05), MDA, IL-5, IL-13 content and cell apoptosis rate were higher than those in control group (P＜0.05). Compared with model group, SOD activity, IFN-γ content and cell survival rate in PDTC and ARIs groups were increased(P＜0.05), MDA, IL-5, IL-13 content and cell apoptosis rate were decreased (P＜0.05). Compared with control group, the expression of NF-κB, NF-κB P65 and P38 MAPK protein in model group was significantly increased(P＜0.05). The expression of NF-κB, NF-κB P65 and P38 MAPK protein in PDTC group and ARIs group were significantly decreased (P＜0.05). Conclusions Aldose reductase inhibitors improve the oxidative stress response of human bronchial epithelial cells induced by house dust mites, promote the proliferation of BEAS-2B cells and inhibit apoptosis. |
| first_indexed | 2024-03-08T15:53:51Z |
| format | Article |
| id | doaj.art-a02f94129a064eb2b50ab8474c3cb05c |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T15:53:51Z |
| publishDate | 2022-04-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-a02f94129a064eb2b50ab8474c3cb05c2024-01-09T02:51:38ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-04-0142461662110.16352/j.issn.1001-6325.2022.04.021Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust miteBAO Min, LIN Mei, ZHEN Hai-ning, HE Fang, DING Min, YUAN Zhu-qing, CHEN Ya-jun, XUE Xin-xin0Department of Respiratory and Critical Care Medicine, Wuhan Third Hospital,Wuhan 430060, ChinaObjective To investigate the inhibition of house dust mite induced oxidative stress with human bronchial epithelial cell line BEAS-2B by aldose reductase inhibitor (ARIs). Methods BEAS-2B was divided into control group, model group, NF-κB inhibitor group (PDTC group) and ARIs intervention group (ARIs group). Oxidative stress reaction (MDA and ROS content, SOD activity) was detected by ELISA. The contents of IL-5, IL-13 and IFN-γ were measured by ELISA. MTT was used to detect cell viability. Cell apoptosis was detected by flow cytometry. The expressions of NF-κB, NF-κB P65 and P38 MAPK proteins were detected by Western blot. Results SOD activity, IFN-γ content and cell survival rate in model group were lower than those in control group (P＜0.05), MDA, IL-5, IL-13 content and cell apoptosis rate were higher than those in control group (P＜0.05). Compared with model group, SOD activity, IFN-γ content and cell survival rate in PDTC and ARIs groups were increased(P＜0.05), MDA, IL-5, IL-13 content and cell apoptosis rate were decreased (P＜0.05). Compared with control group, the expression of NF-κB, NF-κB P65 and P38 MAPK protein in model group was significantly increased(P＜0.05). The expression of NF-κB, NF-κB P65 and P38 MAPK protein in PDTC group and ARIs group were significantly decreased (P＜0.05). Conclusions Aldose reductase inhibitors improve the oxidative stress response of human bronchial epithelial cells induced by house dust mites, promote the proliferation of BEAS-2B cells and inhibit apoptosis.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-616.pdfaldose reductase inhibitor|human bronchial epithelial cells|oxidative stress|house dust mites |
| spellingShingle | BAO Min, LIN Mei, ZHEN Hai-ning, HE Fang, DING Min, YUAN Zhu-qing, CHEN Ya-jun, XUE Xin-xin Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite Jichu yixue yu linchuang aldose reductase inhibitor|human bronchial epithelial cells|oxidative stress|house dust mites |
| title | Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite |
| title_full | Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite |
| title_fullStr | Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite |
| title_full_unstemmed | Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite |
| title_short | Aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line BEAS-2 induced by house dust mite |
| title_sort | aldose reductase inhibitors inhibit the oxidative stress response of human bronchial epithelial cell line beas 2 induced by house dust mite |
| topic | aldose reductase inhibitor|human bronchial epithelial cells|oxidative stress|house dust mites |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-4-616.pdf |
| work_keys_str_mv | AT baominlinmeizhenhaininghefangdingminyuanzhuqingchenyajunxuexinxin aldosereductaseinhibitorsinhibittheoxidativestressresponseofhumanbronchialepithelialcelllinebeas2inducedbyhousedustmite |