Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification
This study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocyte-complexes (COCs) were retrieved using aspiration method from 2-...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2012-05-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/32_2/188-192.pdf |
| _version_ | 1818683650802188288 |
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| author | H. Wahid*, M. Thein1, E.A. El-Hafez2, M.O. Abas3, K. Mohd Azam4, O. Fauziah5, Y. Rosnina and H. Hajarian |
| author_facet | H. Wahid*, M. Thein1, E.A. El-Hafez2, M.O. Abas3, K. Mohd Azam4, O. Fauziah5, Y. Rosnina and H. Hajarian |
| author_sort | H. Wahid*, M. Thein1, E.A. El-Hafez2, M.O. Abas3, K. Mohd Azam4, O. Fauziah5, Y. Rosnina and H. Hajarian |
| collection | DOAJ |
| description | This study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocyte-complexes (COCs) were retrieved using aspiration method from 2-6 mm follicles. In Experiment 1, selected oocytes were randomly divided into 4 treatment groups namely freezing solution-exposed, frozen-thawed, vitrification solution-exposed and vitrified-thawed and then oocytes abnormalities were examined under a stereomicroscope. In Experiment 2, oocytes were randomly allocated to the same grouping as experiment 1 plus control group. Following freezing or vitrification, all oocytes were fixed in glutaraldehyde and processed for transmission electron microscopy. In experiment 1, there was a higher incidence of abnormalities in the frozen-thawed and vitrified-warmed oocytes compared to those in freezing solution and vitrification solution-exposed groups (P<0.05). In experiment 2, there were marked alterations in the perivitelline space, microvilli and vesicles of frozen-thawed and vitrified-warmed oocytes characterized by loss of elasticity and integrity of cytoplasmic processes and microvilli following cooling and warming. In conclusion, ethylene glycol-based freezing and vitrification solutions are suitable choices for cryopreservation of immature oocytes and most organelles are able to retain their normal morphology following cryopreservation and thawing processes. |
| first_indexed | 2024-12-17T10:38:07Z |
| format | Article |
| id | doaj.art-a03938c60754477a8079d3ea5e8ed120 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-17T10:38:07Z |
| publishDate | 2012-05-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-a03938c60754477a8079d3ea5e8ed1202022-12-21T21:52:20ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182012-05-01322188192Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and VitrificationH. Wahid*, M. Thein1, E.A. El-Hafez2, M.O. Abas3, K. Mohd Azam4, O. Fauziah5, Y. Rosnina and H. HajarianThis study was conducted to evaluate the effect of different cryopreservation methods (slow-freezing and vitrification) on structural changes of bovine immature oocytes. Bovine ovaries were collected from local abattoirs. Cumulus-oocyte-complexes (COCs) were retrieved using aspiration method from 2-6 mm follicles. In Experiment 1, selected oocytes were randomly divided into 4 treatment groups namely freezing solution-exposed, frozen-thawed, vitrification solution-exposed and vitrified-thawed and then oocytes abnormalities were examined under a stereomicroscope. In Experiment 2, oocytes were randomly allocated to the same grouping as experiment 1 plus control group. Following freezing or vitrification, all oocytes were fixed in glutaraldehyde and processed for transmission electron microscopy. In experiment 1, there was a higher incidence of abnormalities in the frozen-thawed and vitrified-warmed oocytes compared to those in freezing solution and vitrification solution-exposed groups (P<0.05). In experiment 2, there were marked alterations in the perivitelline space, microvilli and vesicles of frozen-thawed and vitrified-warmed oocytes characterized by loss of elasticity and integrity of cytoplasmic processes and microvilli following cooling and warming. In conclusion, ethylene glycol-based freezing and vitrification solutions are suitable choices for cryopreservation of immature oocytes and most organelles are able to retain their normal morphology following cryopreservation and thawing processes.http://pvj.com.pk/pdf-files/32_2/188-192.pdfBovineCryopreservationOocyteUltrastructureVitrification |
| spellingShingle | H. Wahid*, M. Thein1, E.A. El-Hafez2, M.O. Abas3, K. Mohd Azam4, O. Fauziah5, Y. Rosnina and H. Hajarian Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification Pakistan Veterinary Journal Bovine Cryopreservation Oocyte Ultrastructure Vitrification |
| title | Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification |
| title_full | Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification |
| title_fullStr | Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification |
| title_full_unstemmed | Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification |
| title_short | Structural Changes in Cattle Immature Oocytes Subjected to Slow Freezing and Vitrification |
| title_sort | structural changes in cattle immature oocytes subjected to slow freezing and vitrification |
| topic | Bovine Cryopreservation Oocyte Ultrastructure Vitrification |
| url | http://pvj.com.pk/pdf-files/32_2/188-192.pdf |
| work_keys_str_mv | AT hwahidmthein1eaelhafez2moabas3kmohdazam4ofauziah5yrosninaandhhajarian structuralchangesincattleimmatureoocytessubjectedtoslowfreezingandvitrification |