Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study
Most of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cy...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-04-01
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| Series: | Dose-Response |
| Online Access: | https://doi.org/10.1177/1559325818760880 |
| _version_ | 1828783750109986816 |
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| author | Abdullah A. AlKahtane PhD Saud Alarifi PhD Ahmed A. Al-Qahtani PhD Daoud Ali PhD Suliman Y. Alomar PhD Mohammed S. Aleissia PhD Saad Alkahtani PhD |
| author_facet | Abdullah A. AlKahtane PhD Saud Alarifi PhD Ahmed A. Al-Qahtani PhD Daoud Ali PhD Suliman Y. Alomar PhD Mohammed S. Aleissia PhD Saad Alkahtani PhD |
| author_sort | Abdullah A. AlKahtane PhD |
| collection | DOAJ |
| description | Most of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cypermethrin pesticide on cultured human hepatocarcinoma (HepG2) cells. The HepG2 cells were exposed to cypermethrin (0, 5, 15, 40 ng/mL) for 24 and 48 hours. We observed that cypermethrin caused cell death of HepG2 cells using 3-(4, 5-dimethylthiozolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase tests. Furthermore, cypermethrin reduced HepG2 cells viability in a time and dose dependent basis, that was probably mediated through the induction of reactive oxygen species (ROS) and apoptosis. An increase in ROS generation with a concomitant increase in expression of the proapoptotic protein Bcl-2 and cytochrome c and decrease in the antiapoptosis protein Bax suggested that a mitochondria-mediated pathway was involved in cypermethrin-induced apoptosis. These findings provide insights into the underlying mechanisms involved in cytotoxicity of cypermethrin in HepG2 cells. |
| first_indexed | 2024-12-11T23:17:54Z |
| format | Article |
| id | doaj.art-a039d3da5a0f4ee19d7798db89113e7f |
| institution | Directory Open Access Journal |
| issn | 1559-3258 |
| language | English |
| last_indexed | 2024-12-11T23:17:54Z |
| publishDate | 2018-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Dose-Response |
| spelling | doaj.art-a039d3da5a0f4ee19d7798db89113e7f2022-12-22T00:46:26ZengSAGE PublishingDose-Response1559-32582018-04-011610.1177/1559325818760880Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent StudyAbdullah A. AlKahtane PhD0Saud Alarifi PhD1Ahmed A. Al-Qahtani PhD2Daoud Ali PhD3Suliman Y. Alomar PhD4Mohammed S. Aleissia PhD5Saad Alkahtani PhD6 Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia Department of Microbiology and Immunology, Alfaisal University School of Medicine, Riyadh, Saudi Arabia Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia Department of Biology, Science College, Al-Imam Muhammad Ibn Saud Islamic University, Riyadh, Saudi Arabia Department of Zoology, Science College, King Saud University, Riyadh, Saudi ArabiaMost of the agricultural workers are potentially exposed to pesticides through different routes. Inhalation exposures may result in numerous diseases that can adversely affect an individual’s health and capacity to perform at work. The aim of this study was to determine the cytotoxic potential of cypermethrin pesticide on cultured human hepatocarcinoma (HepG2) cells. The HepG2 cells were exposed to cypermethrin (0, 5, 15, 40 ng/mL) for 24 and 48 hours. We observed that cypermethrin caused cell death of HepG2 cells using 3-(4, 5-dimethylthiozolyl-2)-2,5-diphenyl tetrazolium bromide (MTT) and lactate dehydrogenase tests. Furthermore, cypermethrin reduced HepG2 cells viability in a time and dose dependent basis, that was probably mediated through the induction of reactive oxygen species (ROS) and apoptosis. An increase in ROS generation with a concomitant increase in expression of the proapoptotic protein Bcl-2 and cytochrome c and decrease in the antiapoptosis protein Bax suggested that a mitochondria-mediated pathway was involved in cypermethrin-induced apoptosis. These findings provide insights into the underlying mechanisms involved in cytotoxicity of cypermethrin in HepG2 cells.https://doi.org/10.1177/1559325818760880 |
| spellingShingle | Abdullah A. AlKahtane PhD Saud Alarifi PhD Ahmed A. Al-Qahtani PhD Daoud Ali PhD Suliman Y. Alomar PhD Mohammed S. Aleissia PhD Saad Alkahtani PhD Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study Dose-Response |
| title | Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study |
| title_full | Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study |
| title_fullStr | Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study |
| title_full_unstemmed | Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study |
| title_short | Cytotoxicity and Genotoxicity of Cypermethrin in Hepatocarcinoma Cells: A Dose- and Time-Dependent Study |
| title_sort | cytotoxicity and genotoxicity of cypermethrin in hepatocarcinoma cells a dose and time dependent study |
| url | https://doi.org/10.1177/1559325818760880 |
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