<i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity
Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects. Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro. Setting: Fresh leaves of D. filix-mas wer...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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AOSIS
2017-10-01
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| Series: | Journal of Medicinal Plants for Economic Development |
| Subjects: | |
| Online Access: | https://jomped.org/index.php/jomped/article/view/25 |
| _version_ | 1811272823480516608 |
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| author | Enitome E. Bafor Wellington O. Omokaro Osamuyi H. Uwumarongie Uloma B. Elvis-Offiah Osemelomen Omoruyi Christina V. Viegelmann RuAngelie Edrada-Ebel |
| author_facet | Enitome E. Bafor Wellington O. Omokaro Osamuyi H. Uwumarongie Uloma B. Elvis-Offiah Osemelomen Omoruyi Christina V. Viegelmann RuAngelie Edrada-Ebel |
| author_sort | Enitome E. Bafor |
| collection | DOAJ |
| description | Background: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects.
Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro.
Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria.
Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites.
Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified.
Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect. |
| first_indexed | 2024-04-12T22:47:12Z |
| format | Article |
| id | doaj.art-a03b3cb26f4949429999dd969a7ccc66 |
| institution | Directory Open Access Journal |
| issn | 2519-559X 2616-4809 |
| language | English |
| last_indexed | 2024-04-12T22:47:12Z |
| publishDate | 2017-10-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Journal of Medicinal Plants for Economic Development |
| spelling | doaj.art-a03b3cb26f4949429999dd969a7ccc662022-12-22T03:13:30ZengAOSISJournal of Medicinal Plants for Economic Development2519-559X2616-48092017-10-0111e1e1210.4102/jomped.v1i1.257<i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activityEnitome E. Bafor0Wellington O. Omokaro1Osamuyi H. Uwumarongie2Uloma B. Elvis-Offiah3Osemelomen Omoruyi4Christina V. Viegelmann5RuAngelie Edrada-Ebel6Department of Pharmacology & Toxicology, University of BeninDepartment of Pharmacology & Toxicology, University of BeninDepartment of Pharmacognosy, University of BeninDepartment of Science and Laboratory Technology, University of BeninDepartment of Pharmacology & Toxicology, University of BeninStrathclyde Institute of Pharmacy and Biomedical Sciences, University of StrathclydeStrathclyde Institute of Pharmacy and Biomedical Sciences, University of StrathclydeBackground: The plant Dryopteris filix-mas has been used traditionally for its uterine-stimulant effects. Aim: The current study is therefore aimed at investigating and determining the effect of the leaves of D. filix-mas on uterine contractility in vitro. Setting: Fresh leaves of D. filix-mas were collected from a river bank in the south-western part of Nigeria. Methods: The leaves of D. filix-mas were cleaned, dried and extracted in methanol. The extract (0.07 µg/mL–21.0 µg/mL) was tested on the isolated mouse uteri in order to determine activity on spontaneous-induced uterine contractions. Subsequently the extract (0.005 mg/mL and 0.05 mg/mL) was tested on oxytocin-induced contraction (0.00017 ng/mL–4.98 ng/mL) in calcium-containing media, submaximal oxytocin-induced contraction (0.116 ng/mL) in calcium-free media and in the presence of high KCl-induced uterine contractions (80 mM). The extract was also subjected to mass spectrometric determination of secondary metabolites. Results: The plant extract inhibited spontaneous-induced contractions with IC50 amplitude = 658.41 ng/mL ± 0.11 ng/mL and IC50 frequency = 175.32 ng/mL ± 0.53 ng/mL. The plant extract inhibited oxytocin-induced and high KCl-induced uterine contractions (p < 0.01 at 0.5 mg/mL). The plant extract had no effect on oxytocin-induced contractions under calcium-free conditions. Secondary metabolites belonging to classes of fatty acids, alkaloids, saponin glycosides, amino acids, limonoids, terpenes and porphyrins were identified. Conclusion: The current study reports an inhibitory effect of the plant on uterine contractility in this study, suggesting possible application as a tocolytic or as a contraceptive, as most contraceptive plants have shown uterine-relaxing effect.https://jomped.org/index.php/jomped/article/view/25Dryopteris filix-masuterusoxytocinfatty acidsporphyrins |
| spellingShingle | Enitome E. Bafor Wellington O. Omokaro Osamuyi H. Uwumarongie Uloma B. Elvis-Offiah Osemelomen Omoruyi Christina V. Viegelmann RuAngelie Edrada-Ebel <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity Journal of Medicinal Plants for Economic Development Dryopteris filix-mas uterus oxytocin fatty acids porphyrins |
| title | <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity |
| title_full | <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity |
| title_fullStr | <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity |
| title_full_unstemmed | <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity |
| title_short | <i>Dryopteris filix-mas</i> (Dryopteridaceae) leaves inhibit mouse uterine activity |
| title_sort | i dryopteris filix mas i dryopteridaceae leaves inhibit mouse uterine activity |
| topic | Dryopteris filix-mas uterus oxytocin fatty acids porphyrins |
| url | https://jomped.org/index.php/jomped/article/view/25 |
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