Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice
Background: Previous studies have shown controversial results regarding the pro- or anticonvulsant effects of tramadol. Additionally, the underlying mechanism of seizure induction or alleviation by tramadol has not been fully understood. In the current study, the effects of tramadol on pentylenetet...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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AIMS Press
2022-11-01
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| Series: | AIMS Neuroscience |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2022024?viewType=HTML |
| _version_ | 1797957247627689984 |
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| author | Mazyar Zahir Amir Rashidian Mohsen Hoseini Reyhaneh Akbarian Mohsen Chamanara |
| author_facet | Mazyar Zahir Amir Rashidian Mohsen Hoseini Reyhaneh Akbarian Mohsen Chamanara |
| author_sort | Mazyar Zahir |
| collection | DOAJ |
| description | Background:
Previous studies have shown controversial results regarding the pro- or anticonvulsant effects of tramadol. Additionally, the underlying mechanism of seizure induction or alleviation by tramadol has not been fully understood. In the current study, the effects of tramadol on pentylenetetrazole (PTZ)-induced seizure and the possible involvement of the N-methyl-D-aspartate (NMDA) pathway were assessed in mice.
Methods:
Male Naval Medical Research Institute (NMRI) mice were treated with intravenous infusion of PTZ in order to induce clonic seizures and determine seizure threshold. Tramadol was injected intraperitoneally (0.1–150 mg/kg) 30 minutes prior to elicitation of seizures. The possible effects of intraperitoneal injections of NMDA receptor antagonists, ketamine (0.5 mg/kg) and MK-801 (0.5 mg/kg) on the anticonvulsant property of tramadol were investigated subsequently.
Results:
Tramadol (1–100 mg/kg) increased PTZ-induced seizure threshold in a dose-dependent, time-independent manner, with optimal anticonvulsant effect at a dose of 100 mg/kg. Acute administration of either ketamine (0.5 mg/kg) or MK-801 (0.5 mg/kg) potentiated the anticonvulsant effect of a subeffective dose of tramadol (0.3 mg/kg).
Conclusion:
These results suggest a possible role of the NMDA pathway in the anticonvulsant effect of tramadol. |
| first_indexed | 2024-04-11T00:01:21Z |
| format | Article |
| id | doaj.art-a03c33a7c01345e697986c887a23547b |
| institution | Directory Open Access Journal |
| issn | 2373-7972 |
| language | English |
| last_indexed | 2024-04-11T00:01:21Z |
| publishDate | 2022-11-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Neuroscience |
| spelling | doaj.art-a03c33a7c01345e697986c887a23547b2023-01-10T01:45:54ZengAIMS PressAIMS Neuroscience2373-79722022-11-019444445310.3934/Neuroscience.2022024Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in miceMazyar Zahir 0Amir Rashidian 1Mohsen Hoseini 2Reyhaneh Akbarian3Mohsen Chamanara41. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran 2. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran3. Department of Pharmacology, School of Medicine, Aja University of Medical Sciences, P.O. Box 1411718541, Tehran, IranBackground: Previous studies have shown controversial results regarding the pro- or anticonvulsant effects of tramadol. Additionally, the underlying mechanism of seizure induction or alleviation by tramadol has not been fully understood. In the current study, the effects of tramadol on pentylenetetrazole (PTZ)-induced seizure and the possible involvement of the N-methyl-D-aspartate (NMDA) pathway were assessed in mice. Methods: Male Naval Medical Research Institute (NMRI) mice were treated with intravenous infusion of PTZ in order to induce clonic seizures and determine seizure threshold. Tramadol was injected intraperitoneally (0.1–150 mg/kg) 30 minutes prior to elicitation of seizures. The possible effects of intraperitoneal injections of NMDA receptor antagonists, ketamine (0.5 mg/kg) and MK-801 (0.5 mg/kg) on the anticonvulsant property of tramadol were investigated subsequently. Results: Tramadol (1–100 mg/kg) increased PTZ-induced seizure threshold in a dose-dependent, time-independent manner, with optimal anticonvulsant effect at a dose of 100 mg/kg. Acute administration of either ketamine (0.5 mg/kg) or MK-801 (0.5 mg/kg) potentiated the anticonvulsant effect of a subeffective dose of tramadol (0.3 mg/kg). Conclusion: These results suggest a possible role of the NMDA pathway in the anticonvulsant effect of tramadol.https://www.aimspress.com/article/doi/10.3934/Neuroscience.2022024?viewType=HTMLanticonvulsantsmicen-methylaspartateseizurestramadol |
| spellingShingle | Mazyar Zahir Amir Rashidian Mohsen Hoseini Reyhaneh Akbarian Mohsen Chamanara Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice AIMS Neuroscience anticonvulsants mice n-methylaspartate seizures tramadol |
| title | Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice |
| title_full | Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice |
| title_fullStr | Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice |
| title_full_unstemmed | Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice |
| title_short | Pharmacological evidence for the possible involvement of the NMDA receptor pathway in the anticonvulsant effect of tramadol in mice |
| title_sort | pharmacological evidence for the possible involvement of the nmda receptor pathway in the anticonvulsant effect of tramadol in mice |
| topic | anticonvulsants mice n-methylaspartate seizures tramadol |
| url | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2022024?viewType=HTML |
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