Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD
<p>Abstract</p> <p>Background</p> <p>The essential <it>Escherichia coli </it>gene <it>ygjD </it>belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put <it>ygjD </it...
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| Format: | Article |
| Language: | English |
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BMC
2011-05-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/11/118 |
| _version_ | 1818361837863829504 |
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| author | Ackermann Martin Boehm Alexander Peña-Miller Rafael Bergmiller Tobias |
| author_facet | Ackermann Martin Boehm Alexander Peña-Miller Rafael Bergmiller Tobias |
| author_sort | Ackermann Martin |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The essential <it>Escherichia coli </it>gene <it>ygjD </it>belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put <it>ygjD </it>under control of an inducible promoter, and used time-lapse microscopy and single cell analysis to investigate the phenotypic consequences of the depletion of YgjD protein from growing cells.</p> <p>Results</p> <p>We show that loss of YgjD leads to a marked decrease in cell size and termination of cell division. The transition towards smaller size occurs in a controlled manner: cell elongation and cell division remain coupled, but cell size at division decreases. We also find evidence that depletion of YgjD leads to the synthesis of the intracellular signaling molecule (p)ppGpp, inducing a cellular reaction resembling the stringent response. Concomitant deletion of the <it>relA </it>and <it>spoT </it>genes - leading to a strain that is uncapable of synthesizing (p)ppGpp - abrogates the decrease in cell size, but does not prevent termination of cell division upon YgjD depletion.</p> <p>Conclusions</p> <p>Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. The combination of single-cell timelapse microscopy and statistical analysis can give detailed insights into the phenotypic consequences of the loss of essential genes, and can thus serve as a new tool to study the function of essential genes.</p> |
| first_indexed | 2024-12-13T21:23:02Z |
| format | Article |
| id | doaj.art-a03c7cebfdab4ab18390356d4d48d097 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-13T21:23:02Z |
| publishDate | 2011-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a03c7cebfdab4ab18390356d4d48d0972022-12-21T23:31:03ZengBMCBMC Microbiology1471-21802011-05-0111111810.1186/1471-2180-11-118Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjDAckermann MartinBoehm AlexanderPeña-Miller RafaelBergmiller Tobias<p>Abstract</p> <p>Background</p> <p>The essential <it>Escherichia coli </it>gene <it>ygjD </it>belongs to a universally conserved group of genes whose function has been the focus of a number of recent studies. Here, we put <it>ygjD </it>under control of an inducible promoter, and used time-lapse microscopy and single cell analysis to investigate the phenotypic consequences of the depletion of YgjD protein from growing cells.</p> <p>Results</p> <p>We show that loss of YgjD leads to a marked decrease in cell size and termination of cell division. The transition towards smaller size occurs in a controlled manner: cell elongation and cell division remain coupled, but cell size at division decreases. We also find evidence that depletion of YgjD leads to the synthesis of the intracellular signaling molecule (p)ppGpp, inducing a cellular reaction resembling the stringent response. Concomitant deletion of the <it>relA </it>and <it>spoT </it>genes - leading to a strain that is uncapable of synthesizing (p)ppGpp - abrogates the decrease in cell size, but does not prevent termination of cell division upon YgjD depletion.</p> <p>Conclusions</p> <p>Depletion of YgjD protein from growing cells leads to a decrease in cell size that is contingent on (p)ppGpp, and to a termination of cell division. The combination of single-cell timelapse microscopy and statistical analysis can give detailed insights into the phenotypic consequences of the loss of essential genes, and can thus serve as a new tool to study the function of essential genes.</p>http://www.biomedcentral.com/1471-2180/11/118 |
| spellingShingle | Ackermann Martin Boehm Alexander Peña-Miller Rafael Bergmiller Tobias Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD BMC Microbiology |
| title | Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD |
| title_full | Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD |
| title_fullStr | Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD |
| title_full_unstemmed | Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD |
| title_short | Single-cell time-lapse analysis of depletion of the universally conserved essential protein YgjD |
| title_sort | single cell time lapse analysis of depletion of the universally conserved essential protein ygjd |
| url | http://www.biomedcentral.com/1471-2180/11/118 |
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