Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio
Abstract The potential nutritional value of duckweed Lemna minor (Lemnaceae) was evaluated for common carp Cyprinus carpio fry. Fish were fed diets containing five graded levels of duckweed: 0% (LM0, control), 5% (LM5), 10% (LM10), 15% (LM15) and 20% (LM20). The final weight and specific growth rate...
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| Format: | Article |
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Nature Portfolio
2022-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-07743-x |
| _version_ | 1819236728749162496 |
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| author | Ravi Kumar Goswami JaiGopal Sharma Avanish Kumar Shrivastav Guddu Kumar Brett D. Glencross Douglas R. Tocher Rina Chakrabarti |
| author_facet | Ravi Kumar Goswami JaiGopal Sharma Avanish Kumar Shrivastav Guddu Kumar Brett D. Glencross Douglas R. Tocher Rina Chakrabarti |
| author_sort | Ravi Kumar Goswami |
| collection | DOAJ |
| description | Abstract The potential nutritional value of duckweed Lemna minor (Lemnaceae) was evaluated for common carp Cyprinus carpio fry. Fish were fed diets containing five graded levels of duckweed: 0% (LM0, control), 5% (LM5), 10% (LM10), 15% (LM15) and 20% (LM20). The final weight and specific growth rate were significantly higher in LM15 and LM20 diets fed fish compared to others. Feed conversion ratio was minimum in fish fed diet LM20. Amylase activity was significantly higher in LM0 treatment. Total protease, trypsin and chymotrypsin activities showed linear relationships with the increased level of duckweed in the diet. Protein and essential amino acids contents were significantly higher in carp fed diets LM15 and LM20 compared to others. Lipid content was significantly higher in fish fed duckweed-based diets compared to control. A direct relationship was found between the inclusion level of duckweed in the diet and n-3 long-chain polyunsaturated fatty acid (LC-PUFA) content of carp. Contents of desaturated and elongated products of dietary linolenic acid (18:3n-3) including 20:4n-3, 20:5n-3, 22:5n-3 and 22:6n-3 increased in a graded manner with increasing dietary duckweed. The monounsaturated fatty acids and n-6 PUFA contents reduced significantly in fish fed duckweed. Expression of fads2d6, elovl2, elovl5 and fas were higher in carp fed diets LM10, LM15 and LM20 compared to control fish. The inclusion of L. minor in diet enhanced the nutritional value of carp by increasing protein, lipid, amino acids and n-3 PUFA contents. |
| first_indexed | 2024-12-23T13:09:03Z |
| format | Article |
| id | doaj.art-a03d3ee96a6c456ba61553dd31f64756 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T13:09:03Z |
| publishDate | 2022-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a03d3ee96a6c456ba61553dd31f647562022-12-21T17:45:48ZengNature PortfolioScientific Reports2045-23222022-03-0112111310.1038/s41598-022-07743-xEffect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpioRavi Kumar Goswami0JaiGopal Sharma1Avanish Kumar Shrivastav2Guddu Kumar3Brett D. Glencross4Douglas R. Tocher5Rina Chakrabarti6Aqua Research Lab, Department of Zoology, University of DelhiDepartment of Biotechnology, Delhi Technological UniversityDepartment of Biotechnology, Delhi Technological UniversityAqua Research Lab, Department of Zoology, University of DelhiInstitute of Aquaculture, Faculty of Natural Sciences, University of StirlingInstitute of Aquaculture, Faculty of Natural Sciences, University of StirlingAqua Research Lab, Department of Zoology, University of DelhiAbstract The potential nutritional value of duckweed Lemna minor (Lemnaceae) was evaluated for common carp Cyprinus carpio fry. Fish were fed diets containing five graded levels of duckweed: 0% (LM0, control), 5% (LM5), 10% (LM10), 15% (LM15) and 20% (LM20). The final weight and specific growth rate were significantly higher in LM15 and LM20 diets fed fish compared to others. Feed conversion ratio was minimum in fish fed diet LM20. Amylase activity was significantly higher in LM0 treatment. Total protease, trypsin and chymotrypsin activities showed linear relationships with the increased level of duckweed in the diet. Protein and essential amino acids contents were significantly higher in carp fed diets LM15 and LM20 compared to others. Lipid content was significantly higher in fish fed duckweed-based diets compared to control. A direct relationship was found between the inclusion level of duckweed in the diet and n-3 long-chain polyunsaturated fatty acid (LC-PUFA) content of carp. Contents of desaturated and elongated products of dietary linolenic acid (18:3n-3) including 20:4n-3, 20:5n-3, 22:5n-3 and 22:6n-3 increased in a graded manner with increasing dietary duckweed. The monounsaturated fatty acids and n-6 PUFA contents reduced significantly in fish fed duckweed. Expression of fads2d6, elovl2, elovl5 and fas were higher in carp fed diets LM10, LM15 and LM20 compared to control fish. The inclusion of L. minor in diet enhanced the nutritional value of carp by increasing protein, lipid, amino acids and n-3 PUFA contents.https://doi.org/10.1038/s41598-022-07743-x |
| spellingShingle | Ravi Kumar Goswami JaiGopal Sharma Avanish Kumar Shrivastav Guddu Kumar Brett D. Glencross Douglas R. Tocher Rina Chakrabarti Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio Scientific Reports |
| title | Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio |
| title_full | Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio |
| title_fullStr | Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio |
| title_full_unstemmed | Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio |
| title_short | Effect of Lemna minor supplemented diets on growth, digestive physiology and expression of fatty acids biosynthesis genes of Cyprinus carpio |
| title_sort | effect of lemna minor supplemented diets on growth digestive physiology and expression of fatty acids biosynthesis genes of cyprinus carpio |
| url | https://doi.org/10.1038/s41598-022-07743-x |
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