IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM
ABSTRACT Artificially inoculated asparagus tissue culture plantlets with a virulent fungus, Fusarium proliferatum showed signs of infection as early as 4 days after inoculation. Macroscopic observations revealed presence of early symptoms such as necrotic lesions at the affected area and light...
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2011-11-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/143 |
| _version_ | 1797758585569017856 |
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| author | N.A.R. NIK NORULAINI B. SALLEH R.A.R. ISKANDAR A.K. MOHD OMAR |
| author_facet | N.A.R. NIK NORULAINI B. SALLEH R.A.R. ISKANDAR A.K. MOHD OMAR |
| author_sort | N.A.R. NIK NORULAINI |
| collection | DOAJ |
| description | ABSTRACT
Artificially inoculated asparagus tissue culture plantlets with a virulent fungus, Fusarium
proliferatum showed signs of infection as early as 4 days after inoculation. Macroscopic observations
revealed presence of early symptoms such as necrotic lesions at the affected area and light microscopic
examinations clearly revealed the post-penetration events that took place including the destruction of
surrounding cells. However, little is known of the hyphal activity or advancement on the host's surface at
the initial stage after inoculation. Scanning electron microscopic examination clearly revealed the hyphal
advancement on the surface and the mode of entrance into the host tissues beneath. Four days after
inoculation, the fungi proceeded to spread out from the inoculation point onto the host surface which
eventually developed into a sparse network of both aerial and non-aerial hyphae. Non-aerial hyphae form a
network of mycelium that adheres to the surface and it's movement appeared to be oriented towards the
stomata. Hyphal penetration occurs more often through the stomata, natural openings or wounds. In some
cases, the hyphae crossed over the stomatal opening without entering the host tissues. At places where the
cuticle layer is absent or not well developed the hyphae successfully grew in between the epidermal cells
into the tissues beneath.
Key words: Tissue culture/Asparagus officinalis/shoots/Artificial inoculstion/Fusarium proliferatum. |
| first_indexed | 2024-03-12T18:32:08Z |
| format | Article |
| id | doaj.art-a042049b54e04cb991ef9a5cc69c1beb |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-12T18:32:08Z |
| publishDate | 2011-11-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-a042049b54e04cb991ef9a5cc69c1beb2023-08-02T08:16:36ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2011-11-011210.11598/btb.1999.0.12.143IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUMN.A.R. NIK NORULAINI0B. SALLEH1R.A.R. ISKANDAR2A.K. MOHD OMAR3School of Distance Education,School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia1 School of Industrial Technology,School of Industrial TechnologyABSTRACT Artificially inoculated asparagus tissue culture plantlets with a virulent fungus, Fusarium proliferatum showed signs of infection as early as 4 days after inoculation. Macroscopic observations revealed presence of early symptoms such as necrotic lesions at the affected area and light microscopic examinations clearly revealed the post-penetration events that took place including the destruction of surrounding cells. However, little is known of the hyphal activity or advancement on the host's surface at the initial stage after inoculation. Scanning electron microscopic examination clearly revealed the hyphal advancement on the surface and the mode of entrance into the host tissues beneath. Four days after inoculation, the fungi proceeded to spread out from the inoculation point onto the host surface which eventually developed into a sparse network of both aerial and non-aerial hyphae. Non-aerial hyphae form a network of mycelium that adheres to the surface and it's movement appeared to be oriented towards the stomata. Hyphal penetration occurs more often through the stomata, natural openings or wounds. In some cases, the hyphae crossed over the stomatal opening without entering the host tissues. At places where the cuticle layer is absent or not well developed the hyphae successfully grew in between the epidermal cells into the tissues beneath. Key words: Tissue culture/Asparagus officinalis/shoots/Artificial inoculstion/Fusarium proliferatum.https://journal.biotrop.org/index.php/biotropia/article/view/143 |
| spellingShingle | N.A.R. NIK NORULAINI B. SALLEH R.A.R. ISKANDAR A.K. MOHD OMAR IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM |
| title_full | IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM |
| title_fullStr | IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM |
| title_full_unstemmed | IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM |
| title_short | IN VITRO INOCULATION OF ASPARAGUS OFFICINALIS TISSUE CULTURE SHOOTS WITH FUSARIUM PROLIFERA TUM |
| title_sort | in vitro inoculation of asparagus officinalis tissue culture shoots with fusarium prolifera tum |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/143 |
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