Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus
Obesity is a significant risk factor for the development of type 2 diabetes mellitus (T2DM). Adipose tissue dysfunction can affect the pool of circulating exosomal miRNAs, driving concomitant disease in obesity. These exosomal miRNAs can reflect adipose tissue functionality, thus serving as prognost...
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MDPI AG
2023-11-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/9/6/67 |
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| author | Kseniia V. Dracheva Irina A. Pobozheva Kristina A. Anisimova Stanislav G. Balandov Maria N. Grunina Zarina M. Hamid Dmitriy I. Vasilevsky Sofya N. Pchelina Valentina V. Miroshnikova |
| author_facet | Kseniia V. Dracheva Irina A. Pobozheva Kristina A. Anisimova Stanislav G. Balandov Maria N. Grunina Zarina M. Hamid Dmitriy I. Vasilevsky Sofya N. Pchelina Valentina V. Miroshnikova |
| author_sort | Kseniia V. Dracheva |
| collection | DOAJ |
| description | Obesity is a significant risk factor for the development of type 2 diabetes mellitus (T2DM). Adipose tissue dysfunction can affect the pool of circulating exosomal miRNAs, driving concomitant disease in obesity. These exosomal miRNAs can reflect adipose tissue functionality, thus serving as prognostic biomarkers for disease monitoring in case of T2DM. In the present study, we conducted NanoString microRNA profiling of extracellular vesicles (EVs) secreted by adipose tissue of obese patients (body mass index (BMI) > 35) without T2DM and nonobese individuals (BMI < 30) as a control group. Functional and pathway enrichment analysis showed that miRNAs associated with obesity in this study were implicated in insulin signaling and insulin resistance biological pathways. Further, these microRNAs were screened in serum EVs in the following groups: (1) obese patients with T2DM, (2) obese patients without T2DM, and (3) nonobese individuals as a control group. has-miR-551b-3p was shown to be downregulated in adipose tissue EVs, as well as in serum EVs, of patients with obesity without T2DM. At the same time, the serum exosomal hsa-miR-551b-3p content was significantly higher in obese patients with T2DM when compared with obese patients without T2DM and may be a potential biomarker of T2DM development in obesity. |
| first_indexed | 2024-03-08T20:29:11Z |
| format | Article |
| id | doaj.art-a04495031db84e9884864059e6262892 |
| institution | Directory Open Access Journal |
| issn | 2311-553X |
| language | English |
| last_indexed | 2024-03-08T20:29:11Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Non-Coding RNA |
| spelling | doaj.art-a04495031db84e9884864059e62628922023-12-22T14:29:24ZengMDPI AGNon-Coding RNA2311-553X2023-11-01966710.3390/ncrna9060067Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes MellitusKseniia V. Dracheva0Irina A. Pobozheva1Kristina A. Anisimova2Stanislav G. Balandov3Maria N. Grunina4Zarina M. Hamid5Dmitriy I. Vasilevsky6Sofya N. Pchelina7Valentina V. Miroshnikova8Petersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaCenter for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint-Petersburg State Medical University, 197022 Saint Petersburg, RussiaCenter for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint-Petersburg State Medical University, 197022 Saint Petersburg, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaCenter for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint-Petersburg State Medical University, 197022 Saint Petersburg, RussiaCenter for Surgical Treatment of Obesity and Metabolic Disorders, Pavlov First Saint-Petersburg State Medical University, 197022 Saint Petersburg, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaPetersburg Nuclear Physics Institute Named by B.P. Konstantinov of National Research Centre “Kurchatov Institute”, 188300 Gatchina, RussiaObesity is a significant risk factor for the development of type 2 diabetes mellitus (T2DM). Adipose tissue dysfunction can affect the pool of circulating exosomal miRNAs, driving concomitant disease in obesity. These exosomal miRNAs can reflect adipose tissue functionality, thus serving as prognostic biomarkers for disease monitoring in case of T2DM. In the present study, we conducted NanoString microRNA profiling of extracellular vesicles (EVs) secreted by adipose tissue of obese patients (body mass index (BMI) > 35) without T2DM and nonobese individuals (BMI < 30) as a control group. Functional and pathway enrichment analysis showed that miRNAs associated with obesity in this study were implicated in insulin signaling and insulin resistance biological pathways. Further, these microRNAs were screened in serum EVs in the following groups: (1) obese patients with T2DM, (2) obese patients without T2DM, and (3) nonobese individuals as a control group. has-miR-551b-3p was shown to be downregulated in adipose tissue EVs, as well as in serum EVs, of patients with obesity without T2DM. At the same time, the serum exosomal hsa-miR-551b-3p content was significantly higher in obese patients with T2DM when compared with obese patients without T2DM and may be a potential biomarker of T2DM development in obesity.https://www.mdpi.com/2311-553X/9/6/67exosomal microRNAsobesitytype 2 diabetes mellitus |
| spellingShingle | Kseniia V. Dracheva Irina A. Pobozheva Kristina A. Anisimova Stanislav G. Balandov Maria N. Grunina Zarina M. Hamid Dmitriy I. Vasilevsky Sofya N. Pchelina Valentina V. Miroshnikova Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus Non-Coding RNA exosomal microRNAs obesity type 2 diabetes mellitus |
| title | Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus |
| title_full | Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus |
| title_fullStr | Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus |
| title_full_unstemmed | Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus |
| title_short | Downregulation of Exosomal hsa-miR-551b-3p in Obesity and Its Link to Type 2 Diabetes Mellitus |
| title_sort | downregulation of exosomal hsa mir 551b 3p in obesity and its link to type 2 diabetes mellitus |
| topic | exosomal microRNAs obesity type 2 diabetes mellitus |
| url | https://www.mdpi.com/2311-553X/9/6/67 |
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