Compartmentalization and transporter engineering strategies for terpenoid synthesis
Abstract Microbial cell factories for terpenoid synthesis form a less expensive and more environment-friendly approach than chemical synthesis and extraction, and are thus being regarded as mainstream research recently. Organelle compartmentalization for terpenoid synthesis has received much attenti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-022-01819-z |
| _version_ | 1818204633907068928 |
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| author | Ke Jin Hongzhi Xia Yanfeng Liu Jianghua Li Guocheng Du Xueqin Lv Long Liu |
| author_facet | Ke Jin Hongzhi Xia Yanfeng Liu Jianghua Li Guocheng Du Xueqin Lv Long Liu |
| author_sort | Ke Jin |
| collection | DOAJ |
| description | Abstract Microbial cell factories for terpenoid synthesis form a less expensive and more environment-friendly approach than chemical synthesis and extraction, and are thus being regarded as mainstream research recently. Organelle compartmentalization for terpenoid synthesis has received much attention from researchers owing to the diverse physiochemical characteristics of organelles. In this review, we first systematically summarized various compartmentalization strategies utilized in terpenoid production, mainly plant terpenoids, which can provide catalytic reactions with sufficient intermediates and a suitable environment, while bypassing competing metabolic pathways. In addition, because of the limited storage capacity of cells, strategies used for the expansion of specific organelle membranes were discussed. Next, transporter engineering strategies to overcome the cytotoxic effects of terpenoid accumulation were analyzed. Finally, we discussed the future perspectives of compartmentalization and transporter engineering strategies, with the hope of providing theoretical guidance for designing and constructing cell factories for the purpose of terpenoid production. |
| first_indexed | 2024-12-12T03:44:21Z |
| format | Article |
| id | doaj.art-a044bceddd644331aed45f0c9a3b75cb |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-12T03:44:21Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-a044bceddd644331aed45f0c9a3b75cb2022-12-22T00:39:36ZengBMCMicrobial Cell Factories1475-28592022-05-0121111210.1186/s12934-022-01819-zCompartmentalization and transporter engineering strategies for terpenoid synthesisKe Jin0Hongzhi Xia1Yanfeng Liu2Jianghua Li3Guocheng Du4Xueqin Lv5Long Liu6Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityRichen Bioengineering Co., LtdKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityKey Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan UniversityAbstract Microbial cell factories for terpenoid synthesis form a less expensive and more environment-friendly approach than chemical synthesis and extraction, and are thus being regarded as mainstream research recently. Organelle compartmentalization for terpenoid synthesis has received much attention from researchers owing to the diverse physiochemical characteristics of organelles. In this review, we first systematically summarized various compartmentalization strategies utilized in terpenoid production, mainly plant terpenoids, which can provide catalytic reactions with sufficient intermediates and a suitable environment, while bypassing competing metabolic pathways. In addition, because of the limited storage capacity of cells, strategies used for the expansion of specific organelle membranes were discussed. Next, transporter engineering strategies to overcome the cytotoxic effects of terpenoid accumulation were analyzed. Finally, we discussed the future perspectives of compartmentalization and transporter engineering strategies, with the hope of providing theoretical guidance for designing and constructing cell factories for the purpose of terpenoid production.https://doi.org/10.1186/s12934-022-01819-zTerpenoidsCompartmentalizationTransporterMetabolic engineering |
| spellingShingle | Ke Jin Hongzhi Xia Yanfeng Liu Jianghua Li Guocheng Du Xueqin Lv Long Liu Compartmentalization and transporter engineering strategies for terpenoid synthesis Microbial Cell Factories Terpenoids Compartmentalization Transporter Metabolic engineering |
| title | Compartmentalization and transporter engineering strategies for terpenoid synthesis |
| title_full | Compartmentalization and transporter engineering strategies for terpenoid synthesis |
| title_fullStr | Compartmentalization and transporter engineering strategies for terpenoid synthesis |
| title_full_unstemmed | Compartmentalization and transporter engineering strategies for terpenoid synthesis |
| title_short | Compartmentalization and transporter engineering strategies for terpenoid synthesis |
| title_sort | compartmentalization and transporter engineering strategies for terpenoid synthesis |
| topic | Terpenoids Compartmentalization Transporter Metabolic engineering |
| url | https://doi.org/10.1186/s12934-022-01819-z |
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