Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids
Background: Plant-derived pyrrolizidine alkaloids (PAs) in feed cause metabolic disturbances in farm animals resulting in high economic losses worldwide. The molecular pathways affected by these PAs in cells and tissues are not yet fully understood. The objective of the study was to examine the dose...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/15/10/601 |
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| author | Korinna Huber Janine Saltzmann Sven Daenicke |
| author_facet | Korinna Huber Janine Saltzmann Sven Daenicke |
| author_sort | Korinna Huber |
| collection | DOAJ |
| description | Background: Plant-derived pyrrolizidine alkaloids (PAs) in feed cause metabolic disturbances in farm animals resulting in high economic losses worldwide. The molecular pathways affected by these PAs in cells and tissues are not yet fully understood. The objective of the study was to examine the dose-dependent effects of orally applied PAs derived from tansy ragwort in midlactation dairy cows. Methods: Twenty Holstein dairy cows were treated with target exposures of 0, 0.47, 0.95 and 1.91 mg of total PA/kg of body weight/d in control, PA1, PA2 and PA3, respectively, for 28 days. Liver tissue biopsy and plasma and milk samples were taken at day 28 of treatment to assess changes in metabolic pathways. A targeted metabolomics approach was performed to detect the metabolite profiles in all compartments. Results: The PA-affected metabolite profiling in liver tissue, plasma and milk revealed changes in three substrate classes: acylcarnitines (ACs), phosphatidylcholines (PCs) and sphingomyelins (SMs). In addition, in the plasma, amino acid concentrations were affected by PA exposure. Conclusions: PA exposure disturbed liver metabolism at many sites, especially devastating pathways related to energy metabolism and to amino acid utilization, most likely based on mitochondrial oxidative stress. The effects on the milk metabolite profile may have consequences for milk quality. |
| first_indexed | 2024-03-10T20:50:39Z |
| format | Article |
| id | doaj.art-a0493804611c46418df75548c56dcccc |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-10T20:50:39Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-a0493804611c46418df75548c56dcccc2023-11-19T18:22:35ZengMDPI AGToxins2072-66512023-10-01151060110.3390/toxins15100601Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine AlkaloidsKorinna Huber0Janine Saltzmann1Sven Daenicke2Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler-Institute, 38116 Braunschweig, GermanyInstitute of Animal Nutrition, Friedrich-Loeffler-Institute, 38116 Braunschweig, GermanyBackground: Plant-derived pyrrolizidine alkaloids (PAs) in feed cause metabolic disturbances in farm animals resulting in high economic losses worldwide. The molecular pathways affected by these PAs in cells and tissues are not yet fully understood. The objective of the study was to examine the dose-dependent effects of orally applied PAs derived from tansy ragwort in midlactation dairy cows. Methods: Twenty Holstein dairy cows were treated with target exposures of 0, 0.47, 0.95 and 1.91 mg of total PA/kg of body weight/d in control, PA1, PA2 and PA3, respectively, for 28 days. Liver tissue biopsy and plasma and milk samples were taken at day 28 of treatment to assess changes in metabolic pathways. A targeted metabolomics approach was performed to detect the metabolite profiles in all compartments. Results: The PA-affected metabolite profiling in liver tissue, plasma and milk revealed changes in three substrate classes: acylcarnitines (ACs), phosphatidylcholines (PCs) and sphingomyelins (SMs). In addition, in the plasma, amino acid concentrations were affected by PA exposure. Conclusions: PA exposure disturbed liver metabolism at many sites, especially devastating pathways related to energy metabolism and to amino acid utilization, most likely based on mitochondrial oxidative stress. The effects on the milk metabolite profile may have consequences for milk quality.https://www.mdpi.com/2072-6651/15/10/601pyrrolizidine alkaloid exposure<i>Senecio jacobaea</i>metabolite profilingliver metabolismdairy cow |
| spellingShingle | Korinna Huber Janine Saltzmann Sven Daenicke Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids Toxins pyrrolizidine alkaloid exposure <i>Senecio jacobaea</i> metabolite profiling liver metabolism dairy cow |
| title | Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids |
| title_full | Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids |
| title_fullStr | Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids |
| title_full_unstemmed | Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids |
| title_short | Metabolite Profiling in the Liver, Plasma and Milk of Dairy Cows Exposed to Tansy Ragwort (<i>Senecio jacobae</i>) Pyrrolizidine Alkaloids |
| title_sort | metabolite profiling in the liver plasma and milk of dairy cows exposed to tansy ragwort i senecio jacobae i pyrrolizidine alkaloids |
| topic | pyrrolizidine alkaloid exposure <i>Senecio jacobaea</i> metabolite profiling liver metabolism dairy cow |
| url | https://www.mdpi.com/2072-6651/15/10/601 |
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