Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation
Abstract Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (Scherer et al. in J Exp Med 97:695–710, 1953). They not only allow detailed investigation of molecular pathways but are faster and more cost-effectiv...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14507-0 |
| _version_ | 1811343500139036672 |
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| author | Jaya Krishnan Yan Wang Olga Kenzior Huzaifa Hassan Luke Olsen Dai Tsuchiya Alexander Kenzior Robert Peuß Shaolei Xiong Yongfu Wang Chongbei Zhao Nicolas Rohner |
| author_facet | Jaya Krishnan Yan Wang Olga Kenzior Huzaifa Hassan Luke Olsen Dai Tsuchiya Alexander Kenzior Robert Peuß Shaolei Xiong Yongfu Wang Chongbei Zhao Nicolas Rohner |
| author_sort | Jaya Krishnan |
| collection | DOAJ |
| description | Abstract Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (Scherer et al. in J Exp Med 97:695–710, 1953). They not only allow detailed investigation of molecular pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw many emerging model systems strengthening basic science research. However, lack of genetic and molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an interesting new model system for understanding metabolic adaptation. To further enhance the utility of this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling morphotypes. In this study, we provide detailed methodology of the derivation process along with comprehensive biochemical and molecular characterization of the cell lines, which reflect key metabolic traits of cavefish adaptation. We anticipate these cell lines to become a useful resource for the Astyanax community as well as researchers investigating fish biology, comparative physiology, and metabolism. |
| first_indexed | 2024-04-13T19:30:12Z |
| format | Article |
| id | doaj.art-a049fa95d23443f2a3b05ade73763f7d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T19:30:12Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a049fa95d23443f2a3b05ade73763f7d2022-12-22T02:33:13ZengNature PortfolioScientific Reports2045-23222022-06-0112111010.1038/s41598-022-14507-0Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptationJaya Krishnan0Yan Wang1Olga Kenzior2Huzaifa Hassan3Luke Olsen4Dai Tsuchiya5Alexander Kenzior6Robert Peuß7Shaolei Xiong8Yongfu Wang9Chongbei Zhao10Nicolas Rohner11Stowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchStowers Institute for Medical ResearchAbstract Cell lines have become an integral resource and tool for conducting biological experiments ever since the Hela cell line was first developed (Scherer et al. in J Exp Med 97:695–710, 1953). They not only allow detailed investigation of molecular pathways but are faster and more cost-effective than most in vivo approaches. The last decade saw many emerging model systems strengthening basic science research. However, lack of genetic and molecular tools in these newer systems pose many obstacles. Astyanax mexicanus is proving to be an interesting new model system for understanding metabolic adaptation. To further enhance the utility of this system, we developed liver-derived cell lines from both surface-dwelling and cave-dwelling morphotypes. In this study, we provide detailed methodology of the derivation process along with comprehensive biochemical and molecular characterization of the cell lines, which reflect key metabolic traits of cavefish adaptation. We anticipate these cell lines to become a useful resource for the Astyanax community as well as researchers investigating fish biology, comparative physiology, and metabolism.https://doi.org/10.1038/s41598-022-14507-0 |
| spellingShingle | Jaya Krishnan Yan Wang Olga Kenzior Huzaifa Hassan Luke Olsen Dai Tsuchiya Alexander Kenzior Robert Peuß Shaolei Xiong Yongfu Wang Chongbei Zhao Nicolas Rohner Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation Scientific Reports |
| title | Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| title_full | Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| title_fullStr | Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| title_full_unstemmed | Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| title_short | Liver-derived cell lines from cavefish Astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| title_sort | liver derived cell lines from cavefish astyanax mexicanus as an in vitro model for studying metabolic adaptation |
| url | https://doi.org/10.1038/s41598-022-14507-0 |
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