Rapid Detection of Orthopoxviruses
The aim of the study was to develop a sensitive and fast immunochemical test for the detection of orthopoxviruses (OPXV) in the “point of care” format.Materials and methods. The analyses were performed in cultured crude and purifed preparations of vaccinia virus, cowpoxvirus, rabbitpoxvirus and ectr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2021-10-01
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| Series: | Проблемы особо опасных инфекций |
| Subjects: | |
| Online Access: | https://journal.microbe.ru/jour/article/view/1554 |
| _version_ | 1797220747997872128 |
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| author | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko S. N. Yakubitsky Al. A. Sergeev D. N. Shcherbakov |
| author_facet | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko S. N. Yakubitsky Al. A. Sergeev D. N. Shcherbakov |
| author_sort | A. G. Poltavchenko |
| collection | DOAJ |
| description | The aim of the study was to develop a sensitive and fast immunochemical test for the detection of orthopoxviruses (OPXV) in the “point of care” format.Materials and methods. The analyses were performed in cultured crude and purifed preparations of vaccinia virus, cowpoxvirus, rabbitpoxvirus and ectromelia virus, as well as in the blood and tissue suspensions of infected mice and rabbits. OPXV-antigen was detected by one-stage and two-stage protocols of dot-immunoassay based on flat protein arrays using rabbit polyclonal antibodies as capture and detection reagents.Results and discussion. The results show that the detection limit of OPXV is inversely related to the degree of their purifcation. The one-stage (rapid) protocol is specifc and allows detecting OPXV in crude culture samples of the virus and in clinical samples in the range of 104–103 PFU/ml within 39 minutes. Rapid dot-immunoassay can be applied to detect or exclude the presence of a viral threat in samples and can be useful in various aspects of biosafety provision. The simplicity of the one-stage protocol, the possibility to visually account the results and easy interpretation of the results allow the rapid test to be used in the “point of care” format. |
| first_indexed | 2024-03-08T13:41:32Z |
| format | Article |
| id | doaj.art-a04b7b50f87a4546a043ee09d4db26cf |
| institution | Directory Open Access Journal |
| issn | 0370-1069 2658-719X |
| language | Russian |
| last_indexed | 2024-04-24T12:54:28Z |
| publishDate | 2021-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj.art-a04b7b50f87a4546a043ee09d4db26cf2024-04-05T16:47:16ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2021-10-010310611310.21055/0370-1069-2021-3-106-1131315Rapid Detection of OrthopoxvirusesA. G. Poltavchenko0A. V. Ersh1P. V. Filatov2N. D. Ushkalenko3S. N. Yakubitsky4Al. A. Sergeev5D. N. Shcherbakov6State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”The aim of the study was to develop a sensitive and fast immunochemical test for the detection of orthopoxviruses (OPXV) in the “point of care” format.Materials and methods. The analyses were performed in cultured crude and purifed preparations of vaccinia virus, cowpoxvirus, rabbitpoxvirus and ectromelia virus, as well as in the blood and tissue suspensions of infected mice and rabbits. OPXV-antigen was detected by one-stage and two-stage protocols of dot-immunoassay based on flat protein arrays using rabbit polyclonal antibodies as capture and detection reagents.Results and discussion. The results show that the detection limit of OPXV is inversely related to the degree of their purifcation. The one-stage (rapid) protocol is specifc and allows detecting OPXV in crude culture samples of the virus and in clinical samples in the range of 104–103 PFU/ml within 39 minutes. Rapid dot-immunoassay can be applied to detect or exclude the presence of a viral threat in samples and can be useful in various aspects of biosafety provision. The simplicity of the one-stage protocol, the possibility to visually account the results and easy interpretation of the results allow the rapid test to be used in the “point of care” format.https://journal.microbe.ru/jour/article/view/1554orthopoxvirusesrapid detectionflat protein arraysdot-immunoassayfeld condition |
| spellingShingle | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko S. N. Yakubitsky Al. A. Sergeev D. N. Shcherbakov Rapid Detection of Orthopoxviruses Проблемы особо опасных инфекций orthopoxviruses rapid detection flat protein arrays dot-immunoassay feld condition |
| title | Rapid Detection of Orthopoxviruses |
| title_full | Rapid Detection of Orthopoxviruses |
| title_fullStr | Rapid Detection of Orthopoxviruses |
| title_full_unstemmed | Rapid Detection of Orthopoxviruses |
| title_short | Rapid Detection of Orthopoxviruses |
| title_sort | rapid detection of orthopoxviruses |
| topic | orthopoxviruses rapid detection flat protein arrays dot-immunoassay feld condition |
| url | https://journal.microbe.ru/jour/article/view/1554 |
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