Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions
COVID-19 caused by SARS-CoV-2 caused a worldwide crisis, highlighting the importance of preventive measures in infectious diseases control. SARS-CoV-2 can remain infectious on surfaces for up to several weeks; therefore, proper disinfection is required to mitigate the risk of indirect virus spreadin...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/9/1480 |
| _version_ | 1797520404478164992 |
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| author | Natalia Mazur-Panasiuk Pawel Botwina Adrian Kutaj Damian Woszczyna Krzysztof Pyrc |
| author_facet | Natalia Mazur-Panasiuk Pawel Botwina Adrian Kutaj Damian Woszczyna Krzysztof Pyrc |
| author_sort | Natalia Mazur-Panasiuk |
| collection | DOAJ |
| description | COVID-19 caused by SARS-CoV-2 caused a worldwide crisis, highlighting the importance of preventive measures in infectious diseases control. SARS-CoV-2 can remain infectious on surfaces for up to several weeks; therefore, proper disinfection is required to mitigate the risk of indirect virus spreading. Gaseous ozone treatment has received particular attention as an easily accessible disinfection tool. In this study, we evaluated the virucidal effectiveness of gaseous ozone treatment (>7.3 ppm, 2 h) on murine hepatitis virus (MHV)-contaminated stainless-steel surface and PBS-suspended virus under field conditions at ambient (21.8%) and high (49.8–54.2%) relative humidity. Surficial virus was soiled with 0.3 g/L of BSA. Parallelly, a half-hour vaporization with 7.3% hydrogen peroxide was performed on contaminated carriers. The obtained results showed that gaseous ozone, whilst quite effective against suspended virus, was insufficient in sanitizing coronavirus contaminated surfaces, especially under low RH. Increased humidity created more favorable conditions for MHV inactivation, resulting in 2.1 log titre reduction. Vaporization with 7.3% hydrogen peroxide presented much better virucidal performance than ozonation in a similar experimental setup, indicating that its application may be more advantageous regarding gaseous disinfection of surfaces contaminated with other coronaviruses, including SARS-CoV-2. |
| first_indexed | 2024-03-10T07:56:18Z |
| format | Article |
| id | doaj.art-a04d8e0acea04cdc83d5b5f832545271 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T07:56:18Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a04d8e0acea04cdc83d5b5f8325452712023-11-22T11:49:25ZengMDPI AGAntioxidants2076-39212021-09-01109148010.3390/antiox10091480Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field ConditionsNatalia Mazur-Panasiuk0Pawel Botwina1Adrian Kutaj2Damian Woszczyna3Krzysztof Pyrc4Virogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, PolandVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, PolandRescue and Fire Fighting Services no. 6, Regional Headquarters of the State Fire Service in Krakow, Aleksandry 2, 30-837 Krakow, PolandRescue and Fire Fighting Services no. 6, Regional Headquarters of the State Fire Service in Krakow, Aleksandry 2, 30-837 Krakow, PolandVirogenetics Laboratory of Virology, Malopolska Centre of Biotechnology, Jagiellonian University, Gronostajowa 7A, 30-387 Krakow, PolandCOVID-19 caused by SARS-CoV-2 caused a worldwide crisis, highlighting the importance of preventive measures in infectious diseases control. SARS-CoV-2 can remain infectious on surfaces for up to several weeks; therefore, proper disinfection is required to mitigate the risk of indirect virus spreading. Gaseous ozone treatment has received particular attention as an easily accessible disinfection tool. In this study, we evaluated the virucidal effectiveness of gaseous ozone treatment (>7.3 ppm, 2 h) on murine hepatitis virus (MHV)-contaminated stainless-steel surface and PBS-suspended virus under field conditions at ambient (21.8%) and high (49.8–54.2%) relative humidity. Surficial virus was soiled with 0.3 g/L of BSA. Parallelly, a half-hour vaporization with 7.3% hydrogen peroxide was performed on contaminated carriers. The obtained results showed that gaseous ozone, whilst quite effective against suspended virus, was insufficient in sanitizing coronavirus contaminated surfaces, especially under low RH. Increased humidity created more favorable conditions for MHV inactivation, resulting in 2.1 log titre reduction. Vaporization with 7.3% hydrogen peroxide presented much better virucidal performance than ozonation in a similar experimental setup, indicating that its application may be more advantageous regarding gaseous disinfection of surfaces contaminated with other coronaviruses, including SARS-CoV-2.https://www.mdpi.com/2076-3921/10/9/1480SARS-CoV-2disinfectionozonehydrogen peroxidequantitative carrier test |
| spellingShingle | Natalia Mazur-Panasiuk Pawel Botwina Adrian Kutaj Damian Woszczyna Krzysztof Pyrc Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions Antioxidants SARS-CoV-2 disinfection ozone hydrogen peroxide quantitative carrier test |
| title | Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions |
| title_full | Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions |
| title_fullStr | Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions |
| title_full_unstemmed | Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions |
| title_short | Ozone Treatment Is Insufficient to Inactivate SARS-CoV-2 Surrogate under Field Conditions |
| title_sort | ozone treatment is insufficient to inactivate sars cov 2 surrogate under field conditions |
| topic | SARS-CoV-2 disinfection ozone hydrogen peroxide quantitative carrier test |
| url | https://www.mdpi.com/2076-3921/10/9/1480 |
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