Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach
Objective To investigate the effect of improving human sperm quality by novel selection approach based on progressive motility and thermotaxis. Methods The capacitated spermatozoa were placed on one end of strip droplets with different length (2, 5, 8 cm) and different temperature conditions(32 ℃...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-08-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200283.pdf |
| _version_ | 1797365735754825728 |
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| author | RAO Jin-peng, QIU Feng, CAI Yi-ting, WEI Kai, JIN Min, JIN Fan |
| author_facet | RAO Jin-peng, QIU Feng, CAI Yi-ting, WEI Kai, JIN Min, JIN Fan |
| author_sort | RAO Jin-peng, QIU Feng, CAI Yi-ting, WEI Kai, JIN Min, JIN Fan |
| collection | DOAJ |
| description | Objective To investigate the effect of improving human sperm quality by novel selection approach based on progressive motility and thermotaxis. Methods The capacitated spermatozoa were placed on one end of strip droplets with different length (2, 5, 8 cm) and different temperature conditions(32 ℃, 37 ℃ or 32 ℃~37 ℃) after density gradient centrifugation. The spermatozoa were collected from the other end of the droplets after swimming for 60 min. The concentration, motility rate, normal morphology rate and DNA fragment rate of sperm were compared among different distance-temperature-combined selection conditions. Results Under the same tempera-ture condition, the spermatozoa selected by long distance (5, 8 cm) had higher rate of normal morphology picture than the initial one (P＜0.05).With the increase of distance, the sperm motility rate increased slightly, while the concentration of sperm decreased significantly (P＜0.01).The sperm concentration in temperature gradient(32 ℃~37 ℃) was higher than that in no-gradient control groups (32 ℃ and 37 ℃,P＜0.01), while the sperm DNA fragment rate was lower (P＜0.05). Conclusions Through long distance swimming, a tremendous amount of sperm with abnormal morphology were eliminated, but long distance selection would also lead to a sharp drop in sperm concentration. The temperature gradients enrich more sperm with better DNA integrity and normal thermotaxis. Under the condition of 5 cm, 32 ℃~37 ℃, the effect of sperm selection is better, which may reduce the risk of genetic defective sperm combining with oocytes during IVF. |
| first_indexed | 2024-03-08T16:53:12Z |
| format | Article |
| id | doaj.art-a04e1a24e8404bd786603a904dd85ecb |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:12Z |
| publishDate | 2020-08-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-a04e1a24e8404bd786603a904dd85ecb2024-01-05T03:16:59ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-08-0140810251030Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approachRAO Jin-peng, QIU Feng, CAI Yi-ting, WEI Kai, JIN Min, JIN Fan01. Reproductive Medical Center, the Second Affiliated Hospital; ;2. Key Laboratory of Reproductive Genetics, Ministry of Education, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, ChinaObjective To investigate the effect of improving human sperm quality by novel selection approach based on progressive motility and thermotaxis. Methods The capacitated spermatozoa were placed on one end of strip droplets with different length (2, 5, 8 cm) and different temperature conditions(32 ℃, 37 ℃ or 32 ℃~37 ℃) after density gradient centrifugation. The spermatozoa were collected from the other end of the droplets after swimming for 60 min. The concentration, motility rate, normal morphology rate and DNA fragment rate of sperm were compared among different distance-temperature-combined selection conditions. Results Under the same tempera-ture condition, the spermatozoa selected by long distance (5, 8 cm) had higher rate of normal morphology picture than the initial one (P＜0.05).With the increase of distance, the sperm motility rate increased slightly, while the concentration of sperm decreased significantly (P＜0.01).The sperm concentration in temperature gradient(32 ℃~37 ℃) was higher than that in no-gradient control groups (32 ℃ and 37 ℃,P＜0.01), while the sperm DNA fragment rate was lower (P＜0.05). Conclusions Through long distance swimming, a tremendous amount of sperm with abnormal morphology were eliminated, but long distance selection would also lead to a sharp drop in sperm concentration. The temperature gradients enrich more sperm with better DNA integrity and normal thermotaxis. Under the condition of 5 cm, 32 ℃~37 ℃, the effect of sperm selection is better, which may reduce the risk of genetic defective sperm combining with oocytes during IVF.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200283.pdfspermatozoa|swimming distance|thermotaxis|normal morphology|dna fragment |
| spellingShingle | RAO Jin-peng, QIU Feng, CAI Yi-ting, WEI Kai, JIN Min, JIN Fan Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach Jichu yixue yu linchuang spermatozoa|swimming distance|thermotaxis|normal morphology|dna fragment |
| title | Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach |
| title_full | Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach |
| title_fullStr | Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach |
| title_full_unstemmed | Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach |
| title_short | Effect analysis of improving human sperm quality by a novel distance-temperature-combined selection approach |
| title_sort | effect analysis of improving human sperm quality by a novel distance temperature combined selection approach |
| topic | spermatozoa|swimming distance|thermotaxis|normal morphology|dna fragment |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200283.pdf |
| work_keys_str_mv | AT raojinpengqiufengcaiyitingweikaijinminjinfan effectanalysisofimprovinghumanspermqualitybyanoveldistancetemperaturecombinedselectionapproach |