EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE
Objective: to evaluate the anxiolytic effect of Methanolic extract of Tribulus terrestris (MeTt) in Abino mice. Methods: Tribulus terrestris leaves were collected at the Botanical Garden of the Hamdard University and were identified and authenticated from Eastern Medicine Department of Hamdard Univ...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Khyber Medical University
2014-04-01
|
| Series: | Khyber Medical University Journal |
| Online Access: | https://www.kmuj.kmu.edu.pk/article/view/12354 |
| _version_ | 1818618680538300416 |
|---|---|
| author | Saima Ahmed Sualiha Lutfullah Muhammad Asadullah Izhar Ahmed |
| author_facet | Saima Ahmed Sualiha Lutfullah Muhammad Asadullah Izhar Ahmed |
| author_sort | Saima Ahmed |
| collection | DOAJ |
| description | Objective: to evaluate the anxiolytic effect of Methanolic extract of Tribulus terrestris (MeTt) in Abino mice.
Methods: Tribulus terrestris leaves were collected at the Botanical Garden of the Hamdard University and were identified and authenticated from Eastern Medicine Department of Hamdard University Karachi. Abino mice (30-35 g) of either sex were obtained from the Animal House of Dr.HMI Institute of Pharmacology and Herbal Sciences, Hamdard University, Karachi. Anxiolytic activity was determined using light/dark (LD) test which is commonly used in rodent for anti-anxiety-like behavior that is based on an approach/avoidance conflict between the drive to explore novel areas and an aversion to brightly lit, open spaces: Total thirty six (N=36) mice were randomly divided into six groups. For each of the model studied (n=6). The groups include controls (vehicle) and standard drugs (Diazepam, Bupirone, 1mg/kg) and three groups of MeTt (50,100 and 200 mg/kg.).
Results: MeTtat doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the latency of entry into the dark box with peak effect produced at the dose of 50 mg/kg (178±28.8) seconds) compared to control (132±9.1 seconds). The extract at doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the time spent in the light box with peak effect at the dose of 50 mg/kg (178±28.8 seconds) compared to control (132±9.1 seconds). The effect at this dose was not significantly different from that of diazepam (144.5±34.9seconds). |
| first_indexed | 2024-12-16T17:25:26Z |
| format | Article |
| id | doaj.art-a04f0e6146ac4a96986ef95874cc5a00 |
| institution | Directory Open Access Journal |
| issn | 2305-2643 2305-2651 |
| language | English |
| last_indexed | 2024-12-16T17:25:26Z |
| publishDate | 2014-04-01 |
| publisher | Khyber Medical University |
| record_format | Article |
| series | Khyber Medical University Journal |
| spelling | doaj.art-a04f0e6146ac4a96986ef95874cc5a002022-12-21T22:23:04ZengKhyber Medical UniversityKhyber Medical University Journal2305-26432305-26512014-04-0162697212354EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICESaima Ahmed0Sualiha Lutfullah1Muhammad Asadullah2Izhar AhmedAssistant professor Faculty of pharmacy. Hamdard universtyResearch Associate, Faculty of Pharmacy, Hamdard University, Karachi, PakistanAssociate Professor, Hamdard College of Medicine & Dentistry, Hamdard University, Karachi, PakistanObjective: to evaluate the anxiolytic effect of Methanolic extract of Tribulus terrestris (MeTt) in Abino mice. Methods: Tribulus terrestris leaves were collected at the Botanical Garden of the Hamdard University and were identified and authenticated from Eastern Medicine Department of Hamdard University Karachi. Abino mice (30-35 g) of either sex were obtained from the Animal House of Dr.HMI Institute of Pharmacology and Herbal Sciences, Hamdard University, Karachi. Anxiolytic activity was determined using light/dark (LD) test which is commonly used in rodent for anti-anxiety-like behavior that is based on an approach/avoidance conflict between the drive to explore novel areas and an aversion to brightly lit, open spaces: Total thirty six (N=36) mice were randomly divided into six groups. For each of the model studied (n=6). The groups include controls (vehicle) and standard drugs (Diazepam, Bupirone, 1mg/kg) and three groups of MeTt (50,100 and 200 mg/kg.). Results: MeTtat doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the latency of entry into the dark box with peak effect produced at the dose of 50 mg/kg (178±28.8) seconds) compared to control (132±9.1 seconds). The extract at doses of 50-200 mg/kg significantly (p<0.05, 0.01) increased the time spent in the light box with peak effect at the dose of 50 mg/kg (178±28.8 seconds) compared to control (132±9.1 seconds). The effect at this dose was not significantly different from that of diazepam (144.5±34.9seconds).https://www.kmuj.kmu.edu.pk/article/view/12354 |
| spellingShingle | Saima Ahmed Sualiha Lutfullah Muhammad Asadullah Izhar Ahmed EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE Khyber Medical University Journal |
| title | EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE |
| title_full | EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE |
| title_fullStr | EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE |
| title_full_unstemmed | EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE |
| title_short | EFFECTIVENESS OF TRIBULUS TERRESTRIS FOR PSYCHOPHARMACOLOGICAL ACTIVITY ON LIGHT/ DARK BOX IN NMRI MICE |
| title_sort | effectiveness of tribulus terrestris for psychopharmacological activity on light dark box in nmri mice |
| url | https://www.kmuj.kmu.edu.pk/article/view/12354 |
| work_keys_str_mv | AT saimaahmed effectivenessoftribulusterrestrisforpsychopharmacologicalactivityonlightdarkboxinnmrimice AT sualihalutfullah effectivenessoftribulusterrestrisforpsychopharmacologicalactivityonlightdarkboxinnmrimice AT muhammadasadullah effectivenessoftribulusterrestrisforpsychopharmacologicalactivityonlightdarkboxinnmrimice AT izharahmed effectivenessoftribulusterrestrisforpsychopharmacologicalactivityonlightdarkboxinnmrimice |