Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction.
Intrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alteration...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3105022?pdf=render |
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| author | Carlos Menendez-Castro Fabian Fahlbusch Nada Cordasic Kerstin Amann Kathrin Münzel Christian Plank Rainer Wachtveitl Wolfgang Rascher Karl F Hilgers Andrea Hartner |
| author_facet | Carlos Menendez-Castro Fabian Fahlbusch Nada Cordasic Kerstin Amann Kathrin Münzel Christian Plank Rainer Wachtveitl Wolfgang Rascher Karl F Hilgers Andrea Hartner |
| author_sort | Carlos Menendez-Castro |
| collection | DOAJ |
| description | Intrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alterations before the onset of hypertension. A rat IUGR model of maternal protein restriction during gestation was used. Dams were fed low protein (LP; casein 8.4%) or isocaloric normal protein diet (NP; casein 17.2%). The offspring was reduced to six males per litter. Immunohistochemical and real-time PCR analyses were performed in myocardial and vascular tissue of neonates and animals at day 70 of life. In the aortas of newborn IUGR rats expression of connective tissue growth factor (CTGF) was induced 3.2-fold. At day 70 of life, the expression of collagen I was increased 5.6-fold in aortas of IUGR rats. In the hearts of neonate IUGR rats, cell proliferation was more prominent compared to controls. At day 70 the expression of osteopontin was induced 7.2-fold. A 3- to 7-fold increase in the expression of the profibrotic cytokines TGF-β and CTGF as well as of microfibrillar matrix molecules was observed. The myocardial expression and deposition of collagens was more prominent in IUGR animals compared to controls at day 70. In the low-protein diet model, IUGR leads to changes in the expression patterns of profibrotic genes and discrete structural abnormalities of vessels and hearts in adolescence, but, with the exception of CTGF, not as early as at the time of birth. Invasive and non-invasive blood pressure measurements confirmed that IUGR rats were normotensive at the time point investigated and that the changes observed occurred independently of an increased blood pressure. Hence, altered matrix composition of the vascular wall and the myocardium may predispose IUGR animals to cardiovascular disease later in life. |
| first_indexed | 2024-12-19T07:16:08Z |
| format | Article |
| id | doaj.art-a04f6b09bb3c4ce3b9350625455a5ac2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T07:16:08Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a04f6b09bb3c4ce3b9350625455a5ac22022-12-21T20:31:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2036910.1371/journal.pone.0020369Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction.Carlos Menendez-CastroFabian FahlbuschNada CordasicKerstin AmannKathrin MünzelChristian PlankRainer WachtveitlWolfgang RascherKarl F HilgersAndrea HartnerIntrauterine growth restriction (IUGR) is a risk factor for cardiovascular disease in later life. Early structural and functional changes in the cardiovascular system after IUGR may contribute to its pathogenesis. We tested the hypothesis that IUGR leads to primary myocardial and vascular alterations before the onset of hypertension. A rat IUGR model of maternal protein restriction during gestation was used. Dams were fed low protein (LP; casein 8.4%) or isocaloric normal protein diet (NP; casein 17.2%). The offspring was reduced to six males per litter. Immunohistochemical and real-time PCR analyses were performed in myocardial and vascular tissue of neonates and animals at day 70 of life. In the aortas of newborn IUGR rats expression of connective tissue growth factor (CTGF) was induced 3.2-fold. At day 70 of life, the expression of collagen I was increased 5.6-fold in aortas of IUGR rats. In the hearts of neonate IUGR rats, cell proliferation was more prominent compared to controls. At day 70 the expression of osteopontin was induced 7.2-fold. A 3- to 7-fold increase in the expression of the profibrotic cytokines TGF-β and CTGF as well as of microfibrillar matrix molecules was observed. The myocardial expression and deposition of collagens was more prominent in IUGR animals compared to controls at day 70. In the low-protein diet model, IUGR leads to changes in the expression patterns of profibrotic genes and discrete structural abnormalities of vessels and hearts in adolescence, but, with the exception of CTGF, not as early as at the time of birth. Invasive and non-invasive blood pressure measurements confirmed that IUGR rats were normotensive at the time point investigated and that the changes observed occurred independently of an increased blood pressure. Hence, altered matrix composition of the vascular wall and the myocardium may predispose IUGR animals to cardiovascular disease later in life.http://europepmc.org/articles/PMC3105022?pdf=render |
| spellingShingle | Carlos Menendez-Castro Fabian Fahlbusch Nada Cordasic Kerstin Amann Kathrin Münzel Christian Plank Rainer Wachtveitl Wolfgang Rascher Karl F Hilgers Andrea Hartner Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. PLoS ONE |
| title | Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. |
| title_full | Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. |
| title_fullStr | Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. |
| title_full_unstemmed | Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. |
| title_short | Early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction. |
| title_sort | early and late postnatal myocardial and vascular changes in a protein restriction rat model of intrauterine growth restriction |
| url | http://europepmc.org/articles/PMC3105022?pdf=render |
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