Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results
Conventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics...
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MDPI AG
2017-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/17/12/2944 |
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| author | Domingos M. C. Rodrigues Rafaela N. Lopes Marcos A. R. Franco Marcelo M. Werneck Regina C. S. B. Allil |
| author_facet | Domingos M. C. Rodrigues Rafaela N. Lopes Marcos A. R. Franco Marcelo M. Werneck Regina C. S. B. Allil |
| author_sort | Domingos M. C. Rodrigues |
| collection | DOAJ |
| description | Conventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics of an immunosensor based on the evanescent field in plastic optical fibers with macro curvature by comparing experimental with simulated results. The work studies different shapes of evanescent-wave based fiber optic sensors, adopting a computational modeling to evaluate the probes with the best sensitivity. The simulation showed that for a U-Shaped sensor, the best results can be achieved with a sensor of 980 µm diameter by 5.0 mm in curvature for refractive index sensing, whereas the meander-shaped sensor with 250 μm in diameter with radius of curvature of 1.5 mm, showed better sensitivity for either bacteria and refractive index (RI) sensing. Then, an immunosensor was developed, firstly to measure refractive index and after that, functionalized to detect Escherichia coli. Based on the results with the simulation, we conducted studies with a real sensor for RI measurements and for Escherichia coli detection aiming to establish the best diameter and curvature radius in order to obtain an optimized sensor. On comparing the experimental results with predictions made from the modelling, good agreements were obtained. The simulations performed allowed the evaluation of new geometric configurations of biosensors that can be easily constructed and that promise improved sensitivity. |
| first_indexed | 2024-12-10T06:56:38Z |
| format | Article |
| id | doaj.art-a05178bffcc649258d1fdb004f48fcf2 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T06:56:38Z |
| publishDate | 2017-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a05178bffcc649258d1fdb004f48fcf22022-12-22T01:58:25ZengMDPI AGSensors1424-82202017-12-011712294410.3390/s17122944s17122944Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental ResultsDomingos M. C. Rodrigues0Rafaela N. Lopes1Marcos A. R. Franco2Marcelo M. Werneck3Regina C. S. B. Allil4Federal University of Rio de Janeiro (UFRJ), Electrical Engineering Program, Photonics and Instrumentation Laboratory, Rio de Janeiro 21.941-901, BrazilFederal University of Rio de Janeiro (UFRJ), Electrical Engineering Program, Photonics and Instrumentation Laboratory, Rio de Janeiro 21.941-901, BrazilInstitute of Advanced Studies (IEAv), S. José dos Campos 12.228-001, BrazilFederal University of Rio de Janeiro (UFRJ), Electrical Engineering Program, Photonics and Instrumentation Laboratory, Rio de Janeiro 21.941-901, BrazilFederal University of Rio de Janeiro (UFRJ), Electrical Engineering Program, Photonics and Instrumentation Laboratory, Rio de Janeiro 21.941-901, BrazilConventional pathogen detection methods require trained personnel, specialized laboratories and can take days to provide a result. Thus, portable biosensors with rapid detection response are vital for the current needs for in-loco quality assays. In this work the authors analyze the characteristics of an immunosensor based on the evanescent field in plastic optical fibers with macro curvature by comparing experimental with simulated results. The work studies different shapes of evanescent-wave based fiber optic sensors, adopting a computational modeling to evaluate the probes with the best sensitivity. The simulation showed that for a U-Shaped sensor, the best results can be achieved with a sensor of 980 µm diameter by 5.0 mm in curvature for refractive index sensing, whereas the meander-shaped sensor with 250 μm in diameter with radius of curvature of 1.5 mm, showed better sensitivity for either bacteria and refractive index (RI) sensing. Then, an immunosensor was developed, firstly to measure refractive index and after that, functionalized to detect Escherichia coli. Based on the results with the simulation, we conducted studies with a real sensor for RI measurements and for Escherichia coli detection aiming to establish the best diameter and curvature radius in order to obtain an optimized sensor. On comparing the experimental results with predictions made from the modelling, good agreements were obtained. The simulations performed allowed the evaluation of new geometric configurations of biosensors that can be easily constructed and that promise improved sensitivity.https://www.mdpi.com/1424-8220/17/12/2944biosensorimmunosensorE. colifiber optic sensorPOF |
| spellingShingle | Domingos M. C. Rodrigues Rafaela N. Lopes Marcos A. R. Franco Marcelo M. Werneck Regina C. S. B. Allil Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results Sensors biosensor immunosensor E. coli fiber optic sensor POF |
| title | Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results |
| title_full | Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results |
| title_fullStr | Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results |
| title_full_unstemmed | Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results |
| title_short | Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results |
| title_sort | sensitivity analysis of different shapes of a plastic optical fiber based immunosensor for escherichia coli simulation and experimental results |
| topic | biosensor immunosensor E. coli fiber optic sensor POF |
| url | https://www.mdpi.com/1424-8220/17/12/2944 |
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