Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats
Abstract Background Pro-inflammatory cytokines such as interleukin-5 (IL-5) and tumor necrosis factor-alpha (TNF-α) as well as immunoglobulin-E (IgE) appear to play a role in asthma. N-acetylcysteine (NAC), an antioxidant, might have clinical benefits in asthma prevention. The possible preventive ef...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2022-01-01
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| Series: | Beni-Suef University Journal of Basic and Applied Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43088-021-00188-7 |
| _version_ | 1828146433883111424 |
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| author | Maha M. Abdel-Fattah Abeer A. A. Salama Basim A. S. Messiha |
| author_facet | Maha M. Abdel-Fattah Abeer A. A. Salama Basim A. S. Messiha |
| author_sort | Maha M. Abdel-Fattah |
| collection | DOAJ |
| description | Abstract Background Pro-inflammatory cytokines such as interleukin-5 (IL-5) and tumor necrosis factor-alpha (TNF-α) as well as immunoglobulin-E (IgE) appear to play a role in asthma. N-acetylcysteine (NAC), an antioxidant, might have clinical benefits in asthma prevention. The possible preventive effects of NAC against experimentally induced asthma in rats are investigated. The rats were allocated into five groups: a normal control, asthma control, a standard dexamethasone (DEXA, 1 mg/kg, orally) group, and two NAC groups (300 and 500 mg/kg, orally, respectively). Ovalbumin (OVA) sensitization was used to trigger asthma, which was then followed by an intra-nasal challenge. Test gents were administrated for 14 days before the challenge and during the three challenge days (20, 21, and 22). The tidal volume (TV) and peak expiratory flow rate (PEFR) as respiratory functions were determined. The pro-inflammatory cytokines as IL-5 and TNF-α were evaluated in lung homogenate. Serum IgE and absolute eosinophil count (AEC) in bronchoalveolar lavage fluid (BALF) were measured. In addition, the oxidative markers in lung tissue and nitrosative marker in BALF were assessed; finally, lungs were isolated for histopathological study. Results NAC restored lung functions, inhibited the asthma-dependent increase in TNF-α, IL-5, IgE, AEC, nitric oxide, and malondialdehyde levels. NAC further re-established lung glutathione content and superoxide dismutase activity, resulting in milder overall lung pathology. Conclusions Experimental bronchial asthma may be protected by NAC. The anti-asthmatic potential of NAC may be explained by its suppressant influence on IgE antibody formation, pro-inflammatory cytokines production, eosinophil infiltration, and oxidative stress. |
| first_indexed | 2024-04-11T20:47:16Z |
| format | Article |
| id | doaj.art-a0525a51fa5540939f48c6d5c56450e0 |
| institution | Directory Open Access Journal |
| issn | 2314-8543 |
| language | English |
| last_indexed | 2024-04-11T20:47:16Z |
| publishDate | 2022-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Beni-Suef University Journal of Basic and Applied Sciences |
| spelling | doaj.art-a0525a51fa5540939f48c6d5c56450e02022-12-22T04:03:58ZengSpringerOpenBeni-Suef University Journal of Basic and Applied Sciences2314-85432022-01-011111910.1186/s43088-021-00188-7Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized ratsMaha M. Abdel-Fattah0Abeer A. A. Salama1Basim A. S. Messiha2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef UniversityDepartment of Pharmacology, National Research CentreDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Beni-Suef UniversityAbstract Background Pro-inflammatory cytokines such as interleukin-5 (IL-5) and tumor necrosis factor-alpha (TNF-α) as well as immunoglobulin-E (IgE) appear to play a role in asthma. N-acetylcysteine (NAC), an antioxidant, might have clinical benefits in asthma prevention. The possible preventive effects of NAC against experimentally induced asthma in rats are investigated. The rats were allocated into five groups: a normal control, asthma control, a standard dexamethasone (DEXA, 1 mg/kg, orally) group, and two NAC groups (300 and 500 mg/kg, orally, respectively). Ovalbumin (OVA) sensitization was used to trigger asthma, which was then followed by an intra-nasal challenge. Test gents were administrated for 14 days before the challenge and during the three challenge days (20, 21, and 22). The tidal volume (TV) and peak expiratory flow rate (PEFR) as respiratory functions were determined. The pro-inflammatory cytokines as IL-5 and TNF-α were evaluated in lung homogenate. Serum IgE and absolute eosinophil count (AEC) in bronchoalveolar lavage fluid (BALF) were measured. In addition, the oxidative markers in lung tissue and nitrosative marker in BALF were assessed; finally, lungs were isolated for histopathological study. Results NAC restored lung functions, inhibited the asthma-dependent increase in TNF-α, IL-5, IgE, AEC, nitric oxide, and malondialdehyde levels. NAC further re-established lung glutathione content and superoxide dismutase activity, resulting in milder overall lung pathology. Conclusions Experimental bronchial asthma may be protected by NAC. The anti-asthmatic potential of NAC may be explained by its suppressant influence on IgE antibody formation, pro-inflammatory cytokines production, eosinophil infiltration, and oxidative stress.https://doi.org/10.1186/s43088-021-00188-7Bronchial asthmaOvalbuminN-acetylcysteineInterleukin-5Immunoglobulin ERats |
| spellingShingle | Maha M. Abdel-Fattah Abeer A. A. Salama Basim A. S. Messiha Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats Beni-Suef University Journal of Basic and Applied Sciences Bronchial asthma Ovalbumin N-acetylcysteine Interleukin-5 Immunoglobulin E Rats |
| title | Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats |
| title_full | Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats |
| title_fullStr | Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats |
| title_full_unstemmed | Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats |
| title_short | Immunomodulatory and anti-inflammatory effects of N-acetylcysteine in ovalbumin-sensitized rats |
| title_sort | immunomodulatory and anti inflammatory effects of n acetylcysteine in ovalbumin sensitized rats |
| topic | Bronchial asthma Ovalbumin N-acetylcysteine Interleukin-5 Immunoglobulin E Rats |
| url | https://doi.org/10.1186/s43088-021-00188-7 |
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