MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI
In this study, we found miR-362-5p was upregulated in bladder cancer tissues and we predicted that QKI is potential a target of miR-362-5p and MBNL1-AS1 might be able to directly target to miR-362-5p. We attempted to evaluate whether miR-362-5p could play its roles in bladder cancer through regulati...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-03-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2020.00164/full |
| _version_ | 1818923015162822656 |
|---|---|
| author | Xiaosong Wei Beibei Wang Qi Wang Xiaoming Yang Yang Yang Zhiwei Fang Chengzhi Yi Lei Shi Xin Fan Jin Tao Yufeng Guo Dongkui Song |
| author_facet | Xiaosong Wei Beibei Wang Qi Wang Xiaoming Yang Yang Yang Zhiwei Fang Chengzhi Yi Lei Shi Xin Fan Jin Tao Yufeng Guo Dongkui Song |
| author_sort | Xiaosong Wei |
| collection | DOAJ |
| description | In this study, we found miR-362-5p was upregulated in bladder cancer tissues and we predicted that QKI is potential a target of miR-362-5p and MBNL1-AS1 might be able to directly target to miR-362-5p. We attempted to evaluate whether miR-362-5p could play its roles in bladder cancer through regulating QKI (quaking) and whether the expression and function of miR-362-5p could be mediated by lncRNA MBNL1-AS1. We performed the gain- and loss-function experiments to explore the association between miR-362-5p expression and bladder cancer proliferation. In vivo, the nude mice were injected with miR-362-5p knockdown SW780 cells to assess the effects of miR-362-5p on tumor growth. The results showed upregulation of miR-362-5p promoted cell proliferation of bladder cancer cells. MBNL1-AS1 and QKI could directly bind with miR-362-5p, and knockdown of MBNL1-AS1 or QKI could abrogate the regulatory effects of miR-362-5p on bladder cancer cell proliferation. Furthermore, downregulation of miR-362-5p inhibited bladder tumor growth and increased QKI expression. Our data unveiled that miR-362-5p may play an oncogenic role in bladder cancer through QKI and MBNL1-AS1 might function as a sponge to mediate the miR-362-5p expression and function. |
| first_indexed | 2024-12-20T02:02:43Z |
| format | Article |
| id | doaj.art-a055baf1df9e44be95874122517e5bd3 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T02:02:43Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-a055baf1df9e44be95874122517e5bd32022-12-21T19:57:16ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122020-03-011110.3389/fphar.2020.00164491134MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKIXiaosong Wei0Beibei Wang1Qi Wang2Xiaoming Yang3Yang Yang4Zhiwei Fang5Chengzhi Yi6Lei Shi7Xin Fan8Jin Tao9Yufeng Guo10Dongkui Song11Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaCollege of Science, The Australian National University, Canberra, ACT, AustraliaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaIn this study, we found miR-362-5p was upregulated in bladder cancer tissues and we predicted that QKI is potential a target of miR-362-5p and MBNL1-AS1 might be able to directly target to miR-362-5p. We attempted to evaluate whether miR-362-5p could play its roles in bladder cancer through regulating QKI (quaking) and whether the expression and function of miR-362-5p could be mediated by lncRNA MBNL1-AS1. We performed the gain- and loss-function experiments to explore the association between miR-362-5p expression and bladder cancer proliferation. In vivo, the nude mice were injected with miR-362-5p knockdown SW780 cells to assess the effects of miR-362-5p on tumor growth. The results showed upregulation of miR-362-5p promoted cell proliferation of bladder cancer cells. MBNL1-AS1 and QKI could directly bind with miR-362-5p, and knockdown of MBNL1-AS1 or QKI could abrogate the regulatory effects of miR-362-5p on bladder cancer cell proliferation. Furthermore, downregulation of miR-362-5p inhibited bladder tumor growth and increased QKI expression. Our data unveiled that miR-362-5p may play an oncogenic role in bladder cancer through QKI and MBNL1-AS1 might function as a sponge to mediate the miR-362-5p expression and function.https://www.frontiersin.org/article/10.3389/fphar.2020.00164/fullMiR-362-5pbladder cancerQKIlncRNA MBNL1-AS1proliferation |
| spellingShingle | Xiaosong Wei Beibei Wang Qi Wang Xiaoming Yang Yang Yang Zhiwei Fang Chengzhi Yi Lei Shi Xin Fan Jin Tao Yufeng Guo Dongkui Song MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI Frontiers in Pharmacology MiR-362-5p bladder cancer QKI lncRNA MBNL1-AS1 proliferation |
| title | MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI |
| title_full | MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI |
| title_fullStr | MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI |
| title_full_unstemmed | MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI |
| title_short | MiR-362-5p, Which Is Regulated by Long Non-Coding RNA MBNL1-AS1, Promotes the Cell Proliferation and Tumor Growth of Bladder Cancer by Targeting QKI |
| title_sort | mir 362 5p which is regulated by long non coding rna mbnl1 as1 promotes the cell proliferation and tumor growth of bladder cancer by targeting qki |
| topic | MiR-362-5p bladder cancer QKI lncRNA MBNL1-AS1 proliferation |
| url | https://www.frontiersin.org/article/10.3389/fphar.2020.00164/full |
| work_keys_str_mv | AT xiaosongwei mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT beibeiwang mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT qiwang mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT xiaomingyang mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT yangyang mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT zhiweifang mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT chengzhiyi mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT leishi mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT xinfan mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT jintao mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT yufengguo mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki AT dongkuisong mir3625pwhichisregulatedbylongnoncodingrnambnl1as1promotesthecellproliferationandtumorgrowthofbladdercancerbytargetingqki |