Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation
To improve the quality of poor ejaculates, sephadex (FS) and sephadex with ion-exchangers (FS+IE) filtration were used to examine split samples of 18 ejaculates of 6 Karan Fries bulls at various stages of cryopreservation. Data were analyzed using analysis of variance. All semen quality traits impr...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2016-01-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/54981 |
| _version_ | 1797739498617962496 |
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| author | M BHAKAT T K MOHANTY A K GUPTA R KUMAR P R SHIVAHRE M ABDULLAH |
| author_facet | M BHAKAT T K MOHANTY A K GUPTA R KUMAR P R SHIVAHRE M ABDULLAH |
| author_sort | M BHAKAT |
| collection | DOAJ |
| description |
To improve the quality of poor ejaculates, sephadex (FS) and sephadex with ion-exchangers (FS+IE) filtration were used to examine split samples of 18 ejaculates of 6 Karan Fries bulls at various stages of cryopreservation. Data were analyzed using analysis of variance. All semen quality traits improved in both the filtration and further superiority of FS+IE column was observed due to its better efficacy of trapping immotile, dead and abnormal spermatozoa as well as there was decrease in sperm concentration, tail and total abnormalities. The initial, prefreeze and post-thaw motility, viability, sperm cells with intact plasma membranes and sperms with normal acrosomes of FS+IE filtrates were significantly better than the nonfiltered and FS filtered semen samples during different stages of cryopreservation. Therefore, it can be concluded that better quality semen can be harvested from poor ejaculates through FS+IE and FS filtration to improve fertilization potential of the spermatozoa.
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| first_indexed | 2024-03-12T13:58:00Z |
| format | Article |
| id | doaj.art-a05815bdca91479499ac2b95dcc62de5 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-12T13:58:00Z |
| publishDate | 2016-01-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-a05815bdca91479499ac2b95dcc62de52023-08-22T11:15:15ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272016-01-0186110.56093/ijans.v86i1.54981Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservationM BHAKAT0T K MOHANTY1A K GUPTA2R KUMAR3P R SHIVAHRE4M ABDULLAH5ICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 IndiaICAR-National Dairy Research Institute, Karnal, Haryana 132 001 India To improve the quality of poor ejaculates, sephadex (FS) and sephadex with ion-exchangers (FS+IE) filtration were used to examine split samples of 18 ejaculates of 6 Karan Fries bulls at various stages of cryopreservation. Data were analyzed using analysis of variance. All semen quality traits improved in both the filtration and further superiority of FS+IE column was observed due to its better efficacy of trapping immotile, dead and abnormal spermatozoa as well as there was decrease in sperm concentration, tail and total abnormalities. The initial, prefreeze and post-thaw motility, viability, sperm cells with intact plasma membranes and sperms with normal acrosomes of FS+IE filtrates were significantly better than the nonfiltered and FS filtered semen samples during different stages of cryopreservation. Therefore, it can be concluded that better quality semen can be harvested from poor ejaculates through FS+IE and FS filtration to improve fertilization potential of the spermatozoa. https://epubs.icar.org.in/index.php/IJAnS/article/view/54981Crossbred bullsHolstein FriesianSemen analysesSemen quality |
| spellingShingle | M BHAKAT T K MOHANTY A K GUPTA R KUMAR P R SHIVAHRE M ABDULLAH Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation Indian Journal of Animal Sciences Crossbred bulls Holstein Friesian Semen analyses Semen quality |
| title | Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation |
| title_full | Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation |
| title_fullStr | Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation |
| title_full_unstemmed | Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation |
| title_short | Improvement of low grade ejaculates of Holstein Friesian crossbred bulls by different filtration techniques for cryopreservation |
| title_sort | improvement of low grade ejaculates of holstein friesian crossbred bulls by different filtration techniques for cryopreservation |
| topic | Crossbred bulls Holstein Friesian Semen analyses Semen quality |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/54981 |
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