Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions
The interaction between plants and pathogenic microorganisms is a multifaceted process mediated by both plant- and pathogen-derived molecules, including proteins, metabolites, and lipids. Large-scale proteome analysis can quantify the dynamics of proteins, biological pathways, and posttranslational...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/10/1/5 |
| _version_ | 1797443031399399424 |
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| author | Sadegh Balotf Richard Wilson Robert S. Tegg David S. Nichols Calum R. Wilson |
| author_facet | Sadegh Balotf Richard Wilson Robert S. Tegg David S. Nichols Calum R. Wilson |
| author_sort | Sadegh Balotf |
| collection | DOAJ |
| description | The interaction between plants and pathogenic microorganisms is a multifaceted process mediated by both plant- and pathogen-derived molecules, including proteins, metabolites, and lipids. Large-scale proteome analysis can quantify the dynamics of proteins, biological pathways, and posttranslational modifications (PTMs) involved in the plant–pathogen interaction. Mass spectrometry (MS)-based proteomics has become the preferred method for characterizing proteins at the proteome and sub-proteome (e.g., the phosphoproteome) levels. MS-based proteomics can reveal changes in the quantitative state of a proteome and provide a foundation for understanding the mechanisms involved in plant–pathogen interactions. This review is intended as a primer for biologists that may be unfamiliar with the diverse range of methodology for MS-based shotgun proteomics, with a focus on techniques that have been used to investigate plant–pathogen interactions. We provide a summary of the essential steps required for shotgun proteomic studies of plants, pathogens and plant–pathogen interactions, including methods for protein digestion, identification, separation, and quantification. Finally, we discuss how protein PTMs may directly participate in the interaction between a pathogen and its host plant. |
| first_indexed | 2024-03-09T12:50:13Z |
| format | Article |
| id | doaj.art-a05935a6d587473b9dc966f8d788d8de |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-03-09T12:50:13Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-a05935a6d587473b9dc966f8d788d8de2023-11-30T22:08:05ZengMDPI AGProteomes2227-73822022-01-01101510.3390/proteomes10010005Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen InteractionsSadegh Balotf0Richard Wilson1Robert S. Tegg2David S. Nichols3Calum R. Wilson4New Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, New Town, TAS 7008, AustraliaCentral Science Laboratory, University of Tasmania, Hobart, TAS 7001, AustraliaNew Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, New Town, TAS 7008, AustraliaCentral Science Laboratory, University of Tasmania, Hobart, TAS 7001, AustraliaNew Town Research Laboratories, Tasmanian Institute of Agriculture, University of Tasmania, New Town, TAS 7008, AustraliaThe interaction between plants and pathogenic microorganisms is a multifaceted process mediated by both plant- and pathogen-derived molecules, including proteins, metabolites, and lipids. Large-scale proteome analysis can quantify the dynamics of proteins, biological pathways, and posttranslational modifications (PTMs) involved in the plant–pathogen interaction. Mass spectrometry (MS)-based proteomics has become the preferred method for characterizing proteins at the proteome and sub-proteome (e.g., the phosphoproteome) levels. MS-based proteomics can reveal changes in the quantitative state of a proteome and provide a foundation for understanding the mechanisms involved in plant–pathogen interactions. This review is intended as a primer for biologists that may be unfamiliar with the diverse range of methodology for MS-based shotgun proteomics, with a focus on techniques that have been used to investigate plant–pathogen interactions. We provide a summary of the essential steps required for shotgun proteomic studies of plants, pathogens and plant–pathogen interactions, including methods for protein digestion, identification, separation, and quantification. Finally, we discuss how protein PTMs may directly participate in the interaction between a pathogen and its host plant.https://www.mdpi.com/2227-7382/10/1/5mass spectrometryshotgun proteomicsplant–pathogen interactionpost-translational modification |
| spellingShingle | Sadegh Balotf Richard Wilson Robert S. Tegg David S. Nichols Calum R. Wilson Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions Proteomes mass spectrometry shotgun proteomics plant–pathogen interaction post-translational modification |
| title | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions |
| title_full | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions |
| title_fullStr | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions |
| title_full_unstemmed | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions |
| title_short | Shotgun Proteomics as a Powerful Tool for the Study of the Proteomes of Plants, Their Pathogens, and Plant–Pathogen Interactions |
| title_sort | shotgun proteomics as a powerful tool for the study of the proteomes of plants their pathogens and plant pathogen interactions |
| topic | mass spectrometry shotgun proteomics plant–pathogen interaction post-translational modification |
| url | https://www.mdpi.com/2227-7382/10/1/5 |
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